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Science
2012 Feb 17;3356070:848-51. doi: 10.1126/science.1212795.
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RNA editing underlies temperature adaptation in K+ channels from polar octopuses.
Garrett S, Rosenthal JJ.
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To operate in the extreme cold, ion channels from psychrophiles must have evolved structural changes to compensate for their thermal environment. A reasonable assumption would be that the underlying adaptations lie within the encoding genes. Here, we show that delayed rectifier K(+) channel genes from an Antarctic and a tropical octopus encode channels that differ at only four positions and display very similar behavior when expressed in Xenopus oocytes. However, the transcribed messenger RNAs are extensively edited, creating functional diversity. One editing site, which recodes an isoleucine to a valine in the channel's pore, greatly accelerates gating kinetics by destabilizing the open state. This site is extensively edited in both Antarctic and Arctic species, but mostly unedited in tropical species. Thus adenosine-to-inosine RNA editing can respond to the physical environment.
Armstrong,
Destruction of sodium conductance inactivation in squid axons perfused with pronase.
1973, Pubmed
Armstrong,
Destruction of sodium conductance inactivation in squid axons perfused with pronase.
1973,
Pubmed BASILIO,
Synthetic polynucleotides and the amino acid code. V.
1962,
Pubmed Bass,
An unwinding activity that covalently modifies its double-stranded RNA substrate.
1988,
Pubmed
,
Xenbase Bhalla,
Control of human potassium channel inactivation by editing of a small mRNA hairpin.
2004,
Pubmed Burns,
Regulation of serotonin-2C receptor G-protein coupling by RNA editing.
1997,
Pubmed Conti,
Single channel recordings of K+ currents in squid axons.
1980,
Pubmed Cushman,
Voltage dependent activation of potassium channels is coupled to T1 domain structure.
2000,
Pubmed Graveley,
The developmental transcriptome of Drosophila melanogaster.
2011,
Pubmed HODGKIN,
The effect of temperature on the electrical activity of the giant axon of the squid.
1949,
Pubmed HODGKIN,
Currents carried by sodium and potassium ions through the membrane of the giant axon of Loligo.
1952,
Pubmed Katz,
Impact of Antarctic Circumpolar Current development on late Paleogene ocean structure.
2011,
Pubmed Kobertz,
Hanging gondola structure of the T1 domain in a voltage-gated K(+) channel.
2000,
Pubmed
,
Xenbase Kreusch,
Crystal structure of the tetramerization domain of the Shaker potassium channel.
1998,
Pubmed Kukita,
Properties of sodium and potassium channels of the squid giant axon far below 0 degrees C.
1982,
Pubmed Li,
Specification of subunit assembly by the hydrophilic amino-terminal domain of the Shaker potassium channel.
1992,
Pubmed Liman,
Subunit stoichiometry of a mammalian K+ channel determined by construction of multimeric cDNAs.
1992,
Pubmed
,
Xenbase Llano,
Potassium conductance of the squid giant axon. Single-channel studies.
1988,
Pubmed Lomeli,
Control of kinetic properties of AMPA receptor channels by nuclear RNA editing.
1994,
Pubmed
,
Xenbase Long,
Atomic structure of a voltage-dependent K+ channel in a lipid membrane-like environment.
2007,
Pubmed Long,
Crystal structure of a mammalian voltage-dependent Shaker family K+ channel.
2005,
Pubmed Lu,
Coupling between voltage sensors and activation gate in voltage-gated K+ channels.
2002,
Pubmed
,
Xenbase Melcher,
Editing of alpha-amino-3-hydroxy-5-methylisoxazole-4-propionic acid receptor GluR-B pre-mRNA in vitro reveals site-selective adenosine to inosine conversion.
1995,
Pubmed Milescu,
Maximum likelihood estimation of ion channel kinetics from macroscopic currents.
2005,
Pubmed Minor,
The polar T1 interface is linked to conformational changes that open the voltage-gated potassium channel.
2000,
Pubmed
,
Xenbase Ohman,
Biochemistry. A cold editor makes the adaptation.
2012,
Pubmed Pathak,
Closing in on the resting state of the Shaker K(+) channel.
2007,
Pubmed Patton,
RNA editing generates a diverse array of transcripts encoding squid Kv2 K+ channels with altered functional properties.
1997,
Pubmed Qin,
Model-based fitting of single-channel dwell-time distributions.
2004,
Pubmed Reenan,
Molecular determinants and guided evolution of species-specific RNA editing.
2005,
Pubmed Roberson,
An accurate fluorescent assay for quantifying the extent of RNA editing.
2006,
Pubmed Rodríguez,
Voltage gating of Shaker K+ channels. The effect of temperature on ionic and gating currents.
1998,
Pubmed
,
Xenbase Rosenthal,
Molecular identification of SqKv1A. A candidate for the delayed rectifier K channel in squid giant axon.
1996,
Pubmed
,
Xenbase Rosenthal,
Extensive editing of mRNAs for the squid delayed rectifier K+ channel regulates subunit tetramerization.
2002,
Pubmed
,
Xenbase Soler-Llavina,
Functional interactions at the interface between voltage-sensing and pore domains in the Shaker K(v) channel.
2006,
Pubmed
,
Xenbase Taglialatela,
Novel voltage clamp to record small, fast currents from ion channels expressed in Xenopus oocytes.
1992,
Pubmed
,
Xenbase
