Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
???displayArticle.abstract???
The chromosome passenger complex (CPC) consists of Aurora-B kinase and several other subunits. One of these, incenp, binds Aurora-B and regulates its kinase activity. During Xenopus oocyte maturation, incenp accumulates through translation, contributing to aurora-b activation. A previous study has demonstrated that inhibition of incenp translation during oocyte maturation diminishes aurora-b activation but does not interfere with oocyte maturation, characterized by normal maturation-specific cyclin-b phosphorylation, degradation, and resynthesis. Here we have extended these findings, showing that inhibition of incenp translation during oocyte maturation did not interfere with meiosis I or II, as indicated by the normal emission of the first polar body and metaphase II arrest, followed by the successful emission of the second polar body upon parthenogenetic egg activation. Most importantly, however, when transferred to host frogs and subsequently ovulated, the incenp-deficient eggs were fertilized but failed to undergo mitotic cleavage. Thus, translation of incenp during oocyte maturation appears to be part of oocyte cytoplasmic maturation, preparing the egg for the rapid mitosis following fertilization.
Andrésson,
The kinase Eg2 is a component of the Xenopus oocyte progesterone-activated signaling pathway.
1998, Pubmed,
Xenbase
Andrésson,
The kinase Eg2 is a component of the Xenopus oocyte progesterone-activated signaling pathway.
1998,
Pubmed
,
Xenbase Baltus,
In germ cells of mouse embryonic ovaries, the decision to enter meiosis precedes premeiotic DNA replication.
2006,
Pubmed Bolton,
Aurora B kinase exists in a complex with survivin and INCENP and its kinase activity is stimulated by survivin binding and phosphorylation.
2002,
Pubmed
,
Xenbase Booth,
GIPC participates in G protein signaling downstream of insulin-like growth factor 1 receptor.
2002,
Pubmed
,
Xenbase Carmena,
Making the Auroras glow: regulation of Aurora A and B kinase function by interacting proteins.
2009,
Pubmed Carroll,
Ca2+ release and the development of Ca2+ release mechanisms during oocyte maturation: a prelude to fertilization.
1996,
Pubmed Danilchik,
Requirement for microtubules in new membrane formation during cytokinesis of Xenopus embryos.
1998,
Pubmed
,
Xenbase Dumont,
A centriole- and RanGTP-independent spindle assembly pathway in meiosis I of vertebrate oocytes.
2007,
Pubmed
,
Xenbase Eppig,
Capacity of mouse oocytes from preantral follicles to undergo embryogenesis and development to live young after growth, maturation, and fertilization in vitro.
1989,
Pubmed Erhardt,
Genome-wide analysis reveals a cell cycle-dependent mechanism controlling centromere propagation.
2008,
Pubmed Heasman,
Fertilization of cultured Xenopus oocytes and use in studies of maternally inherited molecules.
1991,
Pubmed
,
Xenbase Hu,
Cell polarization during monopolar cytokinesis.
2008,
Pubmed Lane,
The Aurora kinase inhibitor ZM447439 accelerates first meiosis in mouse oocytes by overriding the spindle assembly checkpoint.
2010,
Pubmed Leblanc,
The small GTPase Cdc42 promotes membrane protrusion during polar body emission via ARP2-nucleated actin polymerization.
2011,
Pubmed
,
Xenbase Liu,
Oocyte isolation and enucleation.
2006,
Pubmed
,
Xenbase Ma,
Cdc42 activation couples spindle positioning to first polar body formation in oocyte maturation.
2006,
Pubmed
,
Xenbase Newport,
A major developmental transition in early Xenopus embryos: I. characterization and timing of cellular changes at the midblastula stage.
1982,
Pubmed
,
Xenbase Nutt,
Comparison of morpholino based translational inhibition during the development of Xenopus laevis and Xenopus tropicalis.
2001,
Pubmed
,
Xenbase Pawlowski,
In the beginning: the initiation of meiosis.
2007,
Pubmed Richardson,
Mechanisms guiding primordial germ cell migration: strategies from different organisms.
2010,
Pubmed Ruchaud,
Chromosomal passengers: conducting cell division.
2007,
Pubmed Sharif,
The chromosome passenger complex is required for fidelity of chromosome transmission and cytokinesis in meiosis of mouse oocytes.
2010,
Pubmed Shuda,
Aurora kinase B modulates chromosome alignment in mouse oocytes.
2009,
Pubmed Summerton,
Morpholino antisense oligomers: the case for an RNase H-independent structural type.
1999,
Pubmed Vogt,
Aurora kinase B, epigenetic state of centromeric heterochromatin and chiasma resolution in oocytes.
2009,
Pubmed Yamamoto,
Regulation of the Aurora B chromosome passenger protein complex during oocyte maturation in Xenopus laevis.
2008,
Pubmed
,
Xenbase Zhang,
Polar body emission requires a RhoA contractile ring and Cdc42-mediated membrane protrusion.
2008,
Pubmed
,
Xenbase Zhou,
Antiapoptotic role for ornithine decarboxylase during oocyte maturation.
2009,
Pubmed
,
Xenbase
