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Formation of a bipolar spindle is indispensable for faithful chromosome segregation and cell division. Spindle integrity is largely dependent on the centrosome and the microtubule network. Centrosome protein Cep57 can bundle microtubules in mammalian cells. Its related protein (Cep57R) in Xenopus was characterized as a stabilization factor for microtubule-kinetochore attachment. Here we show that Cep57 is a pericentriolar material (PCM) component. Its interaction with NEDD1 is necessary for the centrosome localization of Cep57. Depletion of Cep57 leads to unaligned chromosomes and a multipolar spindle, which is induced by PCM fragmentation. In the absence of Cep57, centrosomemicrotubule array assembly activity is weakened, and the spindle length and microtubule density decrease. As a spindle microtubule-binding protein, Cep57 is also responsible for the proper organization of the spindle microtubule and localization of spindle pole focusing proteins. Collectively, these results suggest that Cep57, as a NEDD1-binding centrosome component, could function as a spindle pole- and microtubule-stabilizing factor for establishing robust spindle architecture.
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22508265 ???displayArticle.pmcLink???PMC3434346 ???displayArticle.link???Cell Res
Al-Bassam,
MAP2 and tau bind longitudinally along the outer ridges of microtubule protofilaments.
2002, Pubmed
Al-Bassam,
MAP2 and tau bind longitudinally along the outer ridges of microtubule protofilaments.
2002,
Pubmed Andersen,
Spindle assembly and the art of regulating microtubule dynamics by MAPs and Stathmin/Op18.
2000,
Pubmed Andersen,
Proteomic characterization of the human centrosome by protein correlation profiling.
2003,
Pubmed Blagden,
Polar expeditions--provisioning the centrosome for mitosis.
2003,
Pubmed Bossard,
Translokin is an intracellular mediator of FGF-2 trafficking.
2003,
Pubmed Cravchik,
Identification of a novel microtubule-binding domain in microtubule-associated protein 1A (MAP1A).
1994,
Pubmed DeLuca,
hNuf2 inhibition blocks stable kinetochore-microtubule attachment and induces mitotic cell death in HeLa cells.
2002,
Pubmed Emanuele,
Xenopus Cep57 is a novel kinetochore component involved in microtubule attachment.
2007,
Pubmed
,
Xenbase Ganem,
A mechanism linking extra centrosomes to chromosomal instability.
2009,
Pubmed Garrett,
hTPX2 is required for normal spindle morphology and centrosome integrity during vertebrate cell division.
2002,
Pubmed
,
Xenbase Haren,
NEDD1-dependent recruitment of the gamma-tubulin ring complex to the centrosome is necessary for centriole duplication and spindle assembly.
2006,
Pubmed Heald,
Motor function in the mitotic spindle.
2000,
Pubmed Hutchins,
Systematic analysis of human protein complexes identifies chromosome segregation proteins.
2010,
Pubmed Kapoor,
Probing spindle assembly mechanisms with monastrol, a small molecule inhibitor of the mitotic kinesin, Eg5.
2000,
Pubmed
,
Xenbase Kim,
The pericentriolar satellite protein CEP90 is crucial for integrity of the mitotic spindle pole.
2011,
Pubmed Kline-Smith,
Mitotic spindle assembly and chromosome segregation: refocusing on microtubule dynamics.
2004,
Pubmed Kuriyama,
Centrosome replication in hydroxyurea-arrested CHO cells expressing GFP-tagged centrin2.
2007,
Pubmed Kwok,
The rate of bipolar spindle assembly depends on the microtubule-gliding velocity of the mitotic kinesin Eg5.
2004,
Pubmed
,
Xenbase Li,
14-3-3gamma affects dynamics and integrity of glial filaments by binding to phosphorylated GFAP.
2006,
Pubmed Li,
WD-repeat proteins: structure characteristics, biological function, and their involvement in human diseases.
2001,
Pubmed Manning,
A direct interaction with NEDD1 regulates gamma-tubulin recruitment to the centrosome.
2010,
Pubmed Mayer,
Small molecule inhibitor of mitotic spindle bipolarity identified in a phenotype-based screen.
1999,
Pubmed
,
Xenbase Meng,
Anchorage of microtubule minus ends to adherens junctions regulates epithelial cell-cell contacts.
2008,
Pubmed Merdes,
Formation of spindle poles by dynein/dynactin-dependent transport of NuMA.
2000,
Pubmed Meunier,
Pivotal role of translokin/CEP57 in the unconventional secretion versus nuclear translocation of FGF2.
2009,
Pubmed Momotani,
Cep57, a multidomain protein with unique microtubule and centrosomal localization domains.
2008,
Pubmed Oshimori,
The Plk1 target Kizuna stabilizes mitotic centrosomes to ensure spindle bipolarity.
2006,
Pubmed Oshimori,
Cep72 regulates the localization of key centrosomal proteins and proper bipolar spindle formation.
2009,
Pubmed Palazzo,
Centrosome maturation.
2000,
Pubmed Rusan,
Centrosome function: sometimes less is more.
2009,
Pubmed Silk,
Requirements for NuMA in maintenance and establishment of mammalian spindle poles.
2009,
Pubmed Snape,
Mutations in CEP57 cause mosaic variegated aneuploidy syndrome.
2011,
Pubmed Teng,
Synergistic effects of MAP2 and MAP1B knockout in neuronal migration, dendritic outgrowth, and microtubule organization.
2001,
Pubmed Thein,
Astrin is required for the maintenance of sister chromatid cohesion and centrosome integrity.
2007,
Pubmed Wordeman,
MCAK facilitates chromosome movement by promoting kinetochore microtubule turnover.
2007,
Pubmed Yang,
Tastin is required for bipolar spindle assembly and centrosome integrity during mitosis.
2008,
Pubmed Zhang,
Sequential phosphorylation of Nedd1 by Cdk1 and Plk1 is required for targeting of the gammaTuRC to the centrosome.
2009,
Pubmed Zheng,
Nucleation of microtubule assembly by a gamma-tubulin-containing ring complex.
1995,
Pubmed
,
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