Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
???displayArticle.abstract???
Dynamic variations in the structure of chromatin influence virtually all DNA-related processes in eukaryotes and are controlled in part by post-translational modifications of histones. One such modification, the acetylation of lysine 56 (H3K56ac) in the amino-terminal α-helix (αN) of histone H3, has been implicated in the regulation of nucleosome assembly during DNA replication and repair, and nucleosome disassembly during gene transcription. In Saccharomyces cerevisiae, the histone chaperone Rtt106 contributes to the deposition of newly synthesized H3K56ac-carrying H3-H4 complex on replicating DNA, but it is unclear how Rtt106 binds H3-H4 and specifically recognizes H3K56ac as there is no apparent acetylated lysine reader domain in Rtt106. Here, we show that two domains of Rtt106 are involved in a combinatorial recognition of H3-H4. An N-terminal domain homodimerizes and interacts with H3-H4 independently of acetylation while a double pleckstrin-homology (PH) domain binds the K56-containing region of H3. Affinity is markedly enhanced upon acetylation of K56, an effect that is probably due to increased conformational entropy of the αN helix of H3. Our data support a mode of interaction where the N-terminal homodimeric domain of Rtt106 intercalates between the two H3-H4 components of the (H3-H4)(2) tetramer while two double PH domains in the Rtt106 dimer interact with each of the two H3K56ac sites in (H3-H4)(2). We show that the Rtt106-(H3-H4)(2) interaction is important for gene silencing and the DNA damage response.
Adams,
PHENIX: building new software for automated crystallographic structure determination.
2002, Pubmed
Adams,
PHENIX: building new software for automated crystallographic structure determination.
2002,
Pubmed Blomberg,
The PH superfold: a structural scaffold for multiple functions.
1999,
Pubmed Botuyan,
Structural basis of BACH1 phosphopeptide recognition by BRCA1 tandem BRCT domains.
2004,
Pubmed Bowman,
Probing the (H3-H4)2 histone tetramer structure using pulsed EPR spectroscopy combined with site-directed spin labelling.
2010,
Pubmed
,
Xenbase Brünger,
Crystallography & NMR system: A new software suite for macromolecular structure determination.
1998,
Pubmed Chen,
Acetylated lysine 56 on histone H3 drives chromatin assembly after repair and signals for the completion of repair.
2008,
Pubmed Corpet,
Making copies of chromatin: the challenge of nucleosomal organization and epigenetic information.
2009,
Pubmed Das,
CBP/p300-mediated acetylation of histone H3 on lysine 56.
2009,
Pubmed Delaglio,
NMRPipe: a multidimensional spectral processing system based on UNIX pipes.
1995,
Pubmed Dhalluin,
Structure and ligand of a histone acetyltransferase bromodomain.
1999,
Pubmed Emsley,
Coot: model-building tools for molecular graphics.
2004,
Pubmed Ferentz,
NMR spectroscopy: a multifaceted approach to macromolecular structure.
2000,
Pubmed Hendrickson,
Selenomethionyl proteins produced for analysis by multiwavelength anomalous diffraction (MAD): a vehicle for direct determination of three-dimensional structure.
1990,
Pubmed Huang,
Rtt106p is a histone chaperone involved in heterochromatin-mediated silencing.
2005,
Pubmed Huang,
A novel role for histone chaperones CAF-1 and Rtt106p in heterochromatin silencing.
2007,
Pubmed Huang,
Site-specific introduction of an acetyl-lysine mimic into peptides and proteins by cysteine alkylation.
2010,
Pubmed Koradi,
MOLMOL: a program for display and analysis of macromolecular structures.
1996,
Pubmed Kouzarides,
Chromatin modifications and their function.
2007,
Pubmed Li,
Acetylation of histone H3 lysine 56 regulates replication-coupled nucleosome assembly.
2008,
Pubmed Liu,
Structural analysis of Rtt106p reveals a DNA binding role required for heterochromatin silencing.
2010,
Pubmed Luger,
Preparation of nucleosome core particle from recombinant histones.
1999,
Pubmed
,
Xenbase Luger,
Dynamic nucleosomes.
2006,
Pubmed Minor,
HKL-3000: the integration of data reduction and structure solution--from diffraction images to an initial model in minutes.
2006,
Pubmed Murshudov,
Refinement of macromolecular structures by the maximum-likelihood method.
1997,
Pubmed Otwinowski,
Processing of X-ray diffraction data collected in oscillation mode.
1997,
Pubmed Perrakis,
Automated protein model building combined with iterative structure refinement.
1999,
Pubmed Puig,
The tandem affinity purification (TAP) method: a general procedure of protein complex purification.
2001,
Pubmed Sheldrick,
A short history of SHELX.
2008,
Pubmed Storoni,
Likelihood-enhanced fast rotation functions.
2004,
Pubmed Terwilliger,
SOLVE and RESOLVE: automated structure solution and density modification.
2003,
Pubmed Thomas,
Elevated recombination rates in transcriptionally active DNA.
1989,
Pubmed Tjeertes,
Screen for DNA-damage-responsive histone modifications identifies H3K9Ac and H3K56Ac in human cells.
2009,
Pubmed VanDemark,
The structure of the yFACT Pob3-M domain, its interaction with the DNA replication factor RPA, and a potential role in nucleosome deposition.
2006,
Pubmed White,
Structure of the yeast nucleosome core particle reveals fundamental changes in internucleosome interactions.
2001,
Pubmed
,
Xenbase Williams,
Acetylation in the globular core of histone H3 on lysine-56 promotes chromatin disassembly during transcriptional activation.
2008,
Pubmed Xie,
Histone h3 lysine 56 acetylation is linked to the core transcriptional network in human embryonic stem cells.
2009,
Pubmed Yuan,
Histone H3-K56 acetylation is important for genomic stability in mammals.
2009,
Pubmed Zhou,
Chromatin assembly factor 1 interacts with histone H3 methylated at lysine 79 in the processes of epigenetic silencing and DNA repair.
2006,
Pubmed
