Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Nucleus
2012 Jan 01;33:300-11. doi: 10.4161/nucl.20393.
Show Gene links
Show Anatomy links
Nuclear actin depolymerization in transcriptionally active avian and amphibian oocytes leads to collapse of intranuclear structures.
Maslova A, Krasikova A.
???displayArticle.abstract???
Actin, which is normally depleted in the nuclei of somatic cells, accumulates in high amounts in giant nuclei of amphibian oocytes. The supramolecular organization and functions of this nuclear pool of actin in growing vertebrate oocyte are controversial. Here, we investigated the role of nuclear actin in the maintenance of the spatial architecture of intranuclear structures in avian and amphibian growing oocytes. A meshwork of filamentous actin was not detected in freshly isolated or fixed oocyte nuclei of Xenopus, chicken or quail. We found that the actin meshwork inside the oocytenucleus could be induced by phalloidin treatment. Actin polymerization is demonstrated to be required to stabilize the specific spatial organization of nuclear structures in avian and amphibian growing oocytes. In experiments with the actin depolymerizing drugs cytochalasin D and latrunculin A, we showed that disassembly of nuclear actin polymers led to chromosome condensation and their transportation to a limited space within the oocytenucleus. Experimentally induced "collapsing" of chromosomes and nuclear bodies, together with global inhibition of transcription, strongly resembled the process of karyosphere formation during oocyte growth.
Batalova,
[Karyosphere and extrachromosomal nuclear bodies in oocytes of the scorpionfly, Panorpa communis].
2005, Pubmed
Batalova,
[Karyosphere and extrachromosomal nuclear bodies in oocytes of the scorpionfly, Panorpa communis].
2005,
Pubmed Bogolyubov,
Immunogold localization of RNA polymerase II and pre-mRNA splicing factors in Tenebrio molitor oocyte nuclei with special emphasis on karyosphere development.
2001,
Pubmed Bohnsack,
A selective block of nuclear actin export stabilizes the giant nuclei of Xenopus oocytes.
2006,
Pubmed
,
Xenbase Callan,
Lampbrush chromosomes.
1986,
Pubmed Ch'in Suang Hao,
[Characteristics of oogenesis in the chick. I. The extrafollicular period in the development of the oocytes].
1979,
Pubmed Chuang,
Long-range directional movement of an interphase chromosome site.
2006,
Pubmed Clark,
Diffusible and bound actin nuclei of Xenopus laevis oocytes.
1977,
Pubmed
,
Xenbase Clark,
An actin filament matrix in hand-isolated nuclei of X. laevis oocytes.
1979,
Pubmed
,
Xenbase Cooper,
Effects of cytochalasin and phalloidin on actin.
1987,
Pubmed Cullen,
The conserved kinase NHK-1 is essential for mitotic progression and unifying acentrosomal meiotic spindles in Drosophila melanogaster.
2005,
Pubmed Dundr,
Actin-dependent intranuclear repositioning of an active gene locus in vivo.
2007,
Pubmed Farrants,
Chromatin remodelling and actin organisation.
2008,
Pubmed Flanagan,
Cytochalasins block actin filament elongation by binding to high affinity sites associated with F-actin.
1980,
Pubmed Gaginskaya,
Avian lampbrush chromosomes: a powerful tool for exploration of genome expression.
2009,
Pubmed Gall,
Exporting actin.
2006,
Pubmed
,
Xenbase Gard,
Confocal microscopy and 3-D reconstruction of the cytoskeleton of Xenopus oocytes.
1999,
Pubmed
,
Xenbase Gieni,
Actin dynamics and functions in the interphase nucleus: moving toward an understanding of nuclear polymeric actin.
2009,
Pubmed Gonsior,
Conformational difference between nuclear and cytoplasmic actin as detected by a monoclonal antibody.
1999,
Pubmed
,
Xenbase Gounon,
Involvement of contractile proteins in the changes in consistency of oocyte nucleoplasm of the newt Pleurodeles waltlii.
1981,
Pubmed Greenfield,
The oocyte of the domestic chicken shortly after hatching, studied by electron microscopy.
1966,
Pubmed Grummt,
Actin and myosin as transcription factors.
2006,
Pubmed Gruzova,
Karyosphere in oogenesis and intranuclear morphogenesis.
1993,
Pubmed Gruzova,
[The formation of the karyosphere in the oogenesis of insects and amphibians].
1995,
Pubmed Hofmann,
Nuclear actin: to polymerize or not to polymerize.
2006,
Pubmed Hofmann,
Cofactor requirements for nuclear export of Rev response element (RRE)- and constitutive transport element (CTE)-containing retroviral RNAs. An unexpected role for actin.
2001,
Pubmed
,
Xenbase Hutchison,
Lampbrush chromosomes of the chicken, Gallus domesticus.
1987,
Pubmed Jockusch,
Tracking down the different forms of nuclear actin.
2006,
Pubmed Kiseleva,
Actin- and protein-4.1-containing filaments link nuclear pore complexes to subnuclear organelles in Xenopus oocyte nuclei.
2004,
Pubmed
,
Xenbase Krasikova,
Three-dimensional organisation of RNA-processing machinery in avian growing oocyte nucleus.
2012,
Pubmed Kumaran,
Chromatin dynamics and gene positioning.
2008,
Pubmed Lessard,
Two monoclonal antibodies to actin: one muscle selective and one generally reactive.
1988,
Pubmed Mais,
On the formation of amplified nucleoli during early Xenopus oogenesis.
2002,
Pubmed
,
Xenbase Maslova,
[Spatial arrangement of macro-, midi- and microchromosomes in transcriptionally active nuclei of growing oocytes in birds of the order galliformes].
2011,
Pubmed Miyamoto,
Nuclear actin polymerization is required for transcriptional reprogramming of Oct4 by oocytes.
2011,
Pubmed
,
Xenbase Miyamoto,
Nuclear actin and transcriptional activation.
2011,
Pubmed
,
Xenbase Morgan,
Lampbrush chromosomes and associated bodies: new insights into principles of nuclear structure and function.
2002,
Pubmed Morgan,
Working with oocyte nuclei: cytological preparations of active chromatin and nuclear bodies from amphibian germinal vesicles.
2008,
Pubmed
,
Xenbase Mori,
Intracellular transport by an anchored homogeneously contracting F-actin meshwork.
2011,
Pubmed Morozova,
[Changes in Xenopus oocyte nucleus and cytoplasm organization after actin filaments depolymerization by latrunculin].
2008,
Pubmed
,
Xenbase Morton,
Latrunculin alters the actin-monomer subunit interface to prevent polymerization.
2000,
Pubmed Paine,
The oocyte nucleus isolated in oil retains in vivo structure and functions.
1992,
Pubmed
,
Xenbase Parfenov,
Nuclear actin filaments and their topological changes in frog oocytes.
1995,
Pubmed Percipalle,
Actin-associated hnRNP proteins as transacting factors in the control of mRNA transport and localization.
2009,
Pubmed Percipalle,
Genetic connections of the actin cytoskeleton and beyond.
2007,
Pubmed Percipalle,
Molecular functions of nuclear actin in transcription.
2006,
Pubmed Rübsam,
F-actin is a component of the karyosome in neuropteran oocyte nuclei.
2001,
Pubmed Scheer,
Microinjection of actin-binding proteins and actin antibodies demonstrates involvement of nuclear actin in transcription of lampbrush chromosomes.
1984,
Pubmed Solovei,
Avian sex chromosomes in the lampbrush form: the ZW lampbrush bivalents from six species of bird.
1993,
Pubmed Stüven,
Exportin 6: a novel nuclear export receptor that is specific for profilin.actin complexes.
2003,
Pubmed Visa,
Nuclear functions of actin.
2010,
Pubmed Wallace,
Protein incorporation by isolated amphibian oocytes. 3. Optimum incubation conditions.
1973,
Pubmed
,
Xenbase Wulf,
Fluorescent phallotoxin, a tool for the visualization of cellular actin.
1979,
Pubmed
