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Biochem J
2012 Aug 15;4461:47-58. doi: 10.1042/BJ20120467.
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Embryonic poly(A)-binding protein (EPAB) is required for oocyte maturation and female fertility in mice.
Guzeloglu-Kayisli O, Lalioti MD, Aydiner F, Sasson I, Ilbay O, Sakkas D, Lowther KM, Mehlmann LM, Seli E.
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Gene expression during oocyte maturation and early embryogenesis up to zygotic genome activation requires translational activation of maternally-derived mRNAs. EPAB [embryonic poly(A)-binding protein] is the predominant poly(A)-binding protein during this period in Xenopus, mouse and human. In Xenopus oocytes, ePAB stabilizes maternal mRNAs and promotes their translation. To assess the role of EPAB in mammalian reproduction, we generated Epab-knockout mice. Although Epab(-/-) males and Epab(+/-) of both sexes were fertile, Epab(-/-) female mice were infertile, and could not generate embryos or mature oocytes in vivo or in vitro. Epab(-/-) oocytes failed to achieve translational activation of maternally-stored mRNAs upon stimulation of oocyte maturation, including Ccnb1 (cyclin B1) and Dazl (deleted in azoospermia-like) mRNAs. Microinjection of Epab mRNA into Epab(-/-) germinal vesicle stage oocytes did not rescue maturation, suggesting that EPAB is also required for earlier stages of oogenesis. In addition, late antral follicles in the ovaries of Epab(-/-) mice exhibited impaired cumulus expansion, and a 8-fold decrease in ovulation, associated with a significant down-regulation of mRNAs encoding the EGF (epidermal growth factor)-like growth factors Areg (amphiregulin), Ereg (epiregulin) and Btc (betacellulin), and their downstream regulators, Ptgs2 (prostaglandin synthase 2), Has2 (hyaluronan synthase 2) and Tnfaip6 (tumour necrosis factor α-induced protein 6). The findings from the present study indicate that EPAB is necessary for oogenesis, folliculogenesis and female fertility in mice.
Adashi,
Endocrinology of the ovary.
1994,
Pubmed Barnard,
Symplekin and xGLD-2 are required for CPEB-mediated cytoplasmic polyadenylation.
2004,
Pubmed
,
Xenbase Chen,
Genome-wide analysis of translation reveals a critical role for deleted in azoospermia-like (Dazl) at the oocyte-to-zygote transition.
2011,
Pubmed
,
Xenbase Colledge,
Disruption of c-mos causes parthenogenetic development of unfertilized mouse eggs.
1994,
Pubmed
,
Xenbase Davis,
Anovulation in cyclooxygenase-2-deficient mice is restored by prostaglandin E2 and interleukin-1beta.
1999,
Pubmed de Moor,
Mechanisms of translational control by the 3' UTR in development and differentiation.
2005,
Pubmed Diaz,
Oocytes determine cumulus cell lineage in mouse ovarian follicles.
2007,
Pubmed Dinchuk,
Renal abnormalities and an altered inflammatory response in mice lacking cyclooxygenase II.
1995,
Pubmed Eppig,
Intercommunication between mammalian oocytes and companion somatic cells.
1991,
Pubmed Fesquet,
The MO15 gene encodes the catalytic subunit of a protein kinase that activates cdc2 and other cyclin-dependent kinases (CDKs) through phosphorylation of Thr161 and its homologues.
1993,
Pubmed
,
Xenbase Flach,
The transition from maternal to embryonic control in the 2-cell mouse embryo.
1982,
Pubmed Freimann,
EGF-like factor epiregulin and amphiregulin expression is regulated by gonadotropins/cAMP in human ovarian follicular cells.
2004,
Pubmed Friend,
Embryonic poly(A)-binding protein (ePAB) phosphorylation is required for Xenopus oocyte maturation.
2012,
Pubmed
,
Xenbase Fülöp,
Impaired cumulus mucification and female sterility in tumor necrosis factor-induced protein-6 deficient mice.
2003,
Pubmed Gautier,
Purified maturation-promoting factor contains the product of a Xenopus homolog of the fission yeast cell cycle control gene cdc2+.
1988,
Pubmed
,
Xenbase Gautier,
Cyclin is a component of maturation-promoting factor from Xenopus.
1990,
Pubmed
,
Xenbase Gebauer,
Mouse cytoplasmic polyadenylylation element binding protein: an evolutionarily conserved protein that interacts with the cytoplasmic polyadenylylation elements of c-mos mRNA.
1996,
Pubmed
,
Xenbase Gebauer,
Translational control by cytoplasmic polyadenylation of c-mos mRNA is necessary for oocyte maturation in the mouse.
1994,
Pubmed Goldman,
The rodent estrous cycle: characterization of vaginal cytology and its utility in toxicological studies.
2007,
Pubmed Guzeloglu-Kayisli,
Identification and characterization of human embryonic poly(A) binding protein (EPAB).
2008,
Pubmed
,
Xenbase Hake,
CPEB is a specificity factor that mediates cytoplasmic polyadenylation during Xenopus oocyte maturation.
1994,
Pubmed
,
Xenbase Hampl,
Translational regulation of the gradual increase in histone H1 kinase activity in maturing mouse oocytes.
1995,
Pubmed Hashimoto,
Parthenogenetic activation of oocytes in c-mos-deficient mice.
1994,
Pubmed
,
Xenbase Hsieh,
Epidermal growth factor-like growth factors in the follicular fluid: role in oocyte development and maturation.
2009,
Pubmed Huarte,
Transient translational silencing by reversible mRNA deadenylation.
1992,
Pubmed Kim,
RINGO/cdk1 and CPEB mediate poly(A) tail stabilization and translational regulation by ePAB.
2007,
Pubmed
,
Xenbase Kline,
Quantitative microinjection of mouse oocytes and eggs.
2009,
Pubmed Lin,
Dazl deficiency leads to embryonic arrest of germ cell development in XY C57BL/6 mice.
2005,
Pubmed Lundgren,
mik1 and wee1 cooperate in the inhibitory tyrosine phosphorylation of cdc2.
1991,
Pubmed Masciarelli,
Cyclic nucleotide phosphodiesterase 3A-deficient mice as a model of female infertility.
2004,
Pubmed Masui,
Cytoplasmic control of nuclear behavior during meiotic maturation of frog oocytes.
1971,
Pubmed Matova,
Comparative aspects of animal oogenesis.
2001,
Pubmed
,
Xenbase Mehlmann,
The Gs-linked receptor GPR3 maintains meiotic arrest in mammalian oocytes.
2004,
Pubmed Mueller,
Myt1: a membrane-associated inhibitory kinase that phosphorylates Cdc2 on both threonine-14 and tyrosine-15.
1995,
Pubmed
,
Xenbase Nakanishi,
Possible role of mouse poly(A) polymerase mGLD-2 during oocyte maturation.
2006,
Pubmed
,
Xenbase Ochsner,
Disrupted function of tumor necrosis factor-alpha-stimulated gene 6 blocks cumulus cell-oocyte complex expansion.
2003,
Pubmed Ozturk,
Epab and Pabpc1 are differentially expressed during male germ cell development.
2012,
Pubmed Padmanabhan,
Regulated Pumilio-2 binding controls RINGO/Spy mRNA translation and CPEB activation.
2006,
Pubmed
,
Xenbase Park,
EGF-like growth factors as mediators of LH action in the ovulatory follicle.
2004,
Pubmed Parker,
Inactivation of the p34cdc2-cyclin B complex by the human WEE1 tyrosine kinase.
1992,
Pubmed Pasparakis,
TNF-mediated inflammatory skin disease in mice with epidermis-specific deletion of IKK2.
2002,
Pubmed Piccioni,
Translational regulation during oogenesis and early development: the cap-poly(A) tail relationship.
2005,
Pubmed Polanski,
Cyclin synthesis controls the progression of meiotic maturation in mouse oocytes.
1998,
Pubmed Racki,
CPEB controls oocyte growth and follicle development in the mouse.
2006,
Pubmed Radford,
Translational control by cytoplasmic polyadenylation in Xenopus oocytes.
2008,
Pubmed
,
Xenbase Richards,
Ovulation: new dimensions and new regulators of the inflammatory-like response.
2002,
Pubmed Ruggiu,
The mouse Dazla gene encodes a cytoplasmic protein essential for gametogenesis.
1997,
Pubmed Sallés,
Rapid and sensitive analysis of mRNA polyadenylation states by PCR.
1995,
Pubmed
,
Xenbase Sauer,
Inducible gene targeting in mice using the Cre/lox system.
1998,
Pubmed Sekiguchi,
Expression of epiregulin and amphiregulin in the rat ovary.
2004,
Pubmed Seli,
An embryonic poly(A)-binding protein (ePAB) is expressed in mouse oocytes and early preimplantation embryos.
2005,
Pubmed
,
Xenbase Shimada,
Paracrine and autocrine regulation of epidermal growth factor-like factors in cumulus oocyte complexes and granulosa cells: key roles for prostaglandin synthase 2 and progesterone receptor.
2006,
Pubmed Solomon,
CAK, the p34cdc2 activating kinase, contains a protein identical or closely related to p40MO15.
1993,
Pubmed
,
Xenbase Stebbins-Boaz,
CPEB controls the cytoplasmic polyadenylation of cyclin, Cdk2 and c-mos mRNAs and is necessary for oocyte maturation in Xenopus.
1996,
Pubmed
,
Xenbase Tay,
The control of cyclin B1 mRNA translation during mouse oocyte maturation.
2000,
Pubmed Tay,
Germ cell differentiation and synaptonemal complex formation are disrupted in CPEB knockout mice.
2001,
Pubmed Vanderhyden,
Developmental pattern of the secretion of cumulus expansion-enabling factor by mouse oocytes and the role of oocytes in promoting granulosa cell differentiation.
1990,
Pubmed Vasudevan,
Metazoan oocyte and early embryo development program: a progression through translation regulatory cascades.
2006,
Pubmed
,
Xenbase Voeltz,
A novel embryonic poly(A) binding protein, ePAB, regulates mRNA deadenylation in Xenopus egg extracts.
2001,
Pubmed
,
Xenbase Wassarman,
Meiotic maturation of mouse oocytes in vitro: inhibition of maturation at specific stages of nuclear progression.
1976,
Pubmed Wells,
The C-terminal domain of the Cdc2 inhibitory kinase Myt1 interacts with Cdc2 complexes and is required for inhibition of G(2)/M progression.
1999,
Pubmed White,
The nuclear receptor co-repressor nrip1 (RIP140) is essential for female fertility.
2000,
Pubmed Wilkie,
Embryonic poly(A)-binding protein stimulates translation in germ cells.
2005,
Pubmed
,
Xenbase Winston,
Stability of cyclin B protein during meiotic maturation and the first mitotic cell division in mouse oocytes.
1997,
Pubmed
