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Cell Res
2012 Nov 01;2211:1562-75. doi: 10.1038/cr.2012.113.
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Chromatin-bound NLS proteins recruit membrane vesicles and nucleoporins for nuclear envelope assembly via importin-α/β.
Lu Q, Lu Z, Liu Q, Guo L, Ren H, Fu J, Jiang Q, Clarke PR, Zhang C.
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The mechanism for nuclear envelope (NE) assembly is not fully understood. Importin-β and the small GTPase Ran have been implicated in the spatial regulation of NE assembly process. Here we report that chromatin-bound NLS (nuclear localization sequence) proteins provide docking sites for the NE precursor membrane vesicles and nucleoporins via importin-α and -β during NE assembly in Xenopus egg extracts. We show that along with the fast recruitment of the abundant NLS proteins such as nucleoplasmin and histones to the demembranated sperm chromatin in the extracts, importin-α binds the chromatin NLS proteins rapidly. Meanwhile, importin-β binds cytoplasmic NE precursor membrane vesicles and nucleoporins. Through interacting with importin-α on the chromatin NLS proteins, importin-β targets the membrane vesicles and nucleoporins to the chromatin surface. Once encountering Ran-GTP on the chromatin generated by RCC1, importin-β preferentially binds Ran-GTP and releases the membrane vesicles and nucleoporins for NE assembly. NE assembly is disrupted by blocking the interaction between importin-α and NLS proteins with excess soluble NLS proteins or by depletion of importin-β from the extract. Our findings reveal a novel molecular mechanism for NE assembly in Xenopus egg extracts.
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Antonin,
The integral membrane nucleoporin pom121 functionally links nuclear pore complex assembly and nuclear envelope formation.
2005, Pubmed,
Xenbase
Antonin,
The integral membrane nucleoporin pom121 functionally links nuclear pore complex assembly and nuclear envelope formation.
2005,
Pubmed
,
Xenbase Bañuelos,
Activation mechanism of the nuclear chaperone nucleoplasmin: role of the core domain.
2003,
Pubmed
,
Xenbase Bilbao-Cortés,
Ran binds to chromatin by two distinct mechanisms.
2002,
Pubmed
,
Xenbase Clarke,
Spatial and temporal control of nuclear envelope assembly by Ran GTPase.
2004,
Pubmed
,
Xenbase Clarke,
Spatial and temporal coordination of mitosis by Ran GTPase.
2008,
Pubmed Dingwall,
Nucleoplasmin cDNA sequence reveals polyglutamic acid tracts and a cluster of sequences homologous to putative nuclear localization signals.
1987,
Pubmed
,
Xenbase Dingwall,
A polypeptide domain that specifies migration of nucleoplasmin into the nucleus.
1982,
Pubmed
,
Xenbase Fernandez,
MEL-28 is downstream of the Ran cycle and is required for nuclear-envelope function and chromatin maintenance.
2006,
Pubmed Fichtman,
Inner/Outer nuclear membrane fusion in nuclear pore assembly: biochemical demonstration and molecular analysis.
2010,
Pubmed
,
Xenbase Franz,
MEL-28/ELYS is required for the recruitment of nucleoporins to chromatin and postmitotic nuclear pore complex assembly.
2007,
Pubmed
,
Xenbase Gant,
Nuclear assembly.
1997,
Pubmed Gillespie,
ELYS/MEL-28 chromatin association coordinates nuclear pore complex assembly and replication licensing.
2007,
Pubmed
,
Xenbase Goldfarb,
Importin alpha: a multipurpose nuclear-transport receptor.
2004,
Pubmed Görlich,
Distinct functions for the two importin subunits in nuclear protein import.
1995,
Pubmed
,
Xenbase Gruss,
Ran induces spindle assembly by reversing the inhibitory effect of importin alpha on TPX2 activity.
2001,
Pubmed
,
Xenbase Hachet,
Importin alpha associates with membranes and participates in nuclear envelope assembly in vitro.
2004,
Pubmed
,
Xenbase Harel,
Importin beta negatively regulates nuclear membrane fusion and nuclear pore complex assembly.
2003,
Pubmed
,
Xenbase Hartl,
Nuclear assembly with lambda DNA in fractionated Xenopus egg extracts: an unexpected role for glycogen in formation of a higher order chromatin intermediate.
1994,
Pubmed
,
Xenbase Hetzer,
GTP hydrolysis by Ran is required for nuclear envelope assembly.
2000,
Pubmed
,
Xenbase Hetzer,
Border control at the nucleus: biogenesis and organization of the nuclear membrane and pore complexes.
2009,
Pubmed Hierro,
Electrostatic interactions at the C-terminal domain of nucleoplasmin modulate its chromatin decondensation activity.
2002,
Pubmed
,
Xenbase Hughes,
The role of the ran GTPase in nuclear assembly and DNA replication: characterisation of the effects of Ran mutants.
1998,
Pubmed
,
Xenbase Hutchins,
Dynamic localisation of Ran GTPase during the cell cycle.
2009,
Pubmed Laskey,
The role of nucleoplasmin in chromatin assembly and disassembly.
1993,
Pubmed
,
Xenbase Liu,
Pericentrin contains five NESs and an NLS essential for its nucleocytoplasmic trafficking during the cell cycle.
2010,
Pubmed Lu,
Nucleoplasmin regulates chromatin condensation during apoptosis.
2005,
Pubmed
,
Xenbase Lu,
Requirement for lamin B receptor and its regulation by importin {beta} and phosphorylation in nuclear envelope assembly during mitotic exit.
2010,
Pubmed
,
Xenbase Ma,
Lamin B receptor plays a role in stimulating nuclear envelope production and targeting membrane vesicles to chromatin during nuclear envelope assembly through direct interaction with importin beta.
2007,
Pubmed
,
Xenbase Merdes,
A complex of NuMA and cytoplasmic dynein is essential for mitotic spindle assembly.
1996,
Pubmed
,
Xenbase Mitchell,
Pom121 links two essential subcomplexes of the nuclear pore complex core to the membrane.
2010,
Pubmed Philpott,
Nucleoplasmin remodels sperm chromatin in Xenopus egg extracts.
1992,
Pubmed
,
Xenbase Philpott,
Sperm decondensation in Xenopus egg cytoplasm is mediated by nucleoplasmin.
1991,
Pubmed
,
Xenbase Rasala,
Capture of AT-rich chromatin by ELYS recruits POM121 and NDC1 to initiate nuclear pore assembly.
2008,
Pubmed
,
Xenbase Rasala,
ELYS is a dual nucleoporin/kinetochore protein required for nuclear pore assembly and proper cell division.
2006,
Pubmed
,
Xenbase Rice,
Nucleoplasmin-mediated decondensation of Mytilus sperm chromatin. Identification and partial characterization of a nucleoplasmin-like protein with sperm-nuclei decondensing activity in Mytilus californianus.
1995,
Pubmed
,
Xenbase Ryan,
The Ran GTPase cycle is required for yeast nuclear pore complex assembly.
2003,
Pubmed Vasu,
Nuclear pores and nuclear assembly.
2001,
Pubmed Walther,
RanGTP mediates nuclear pore complex assembly.
2003,
Pubmed
,
Xenbase Walther,
The conserved Nup107-160 complex is critical for nuclear pore complex assembly.
2003,
Pubmed
,
Xenbase Wiese,
Role of importin-beta in coupling Ran to downstream targets in microtubule assembly.
2001,
Pubmed
,
Xenbase Yang,
NuMA: an unusually long coiled-coil related protein in the mammalian nucleus.
1992,
Pubmed Zhang,
Roles of Ran-GTP and Ran-GDP in precursor vesicle recruitment and fusion during nuclear envelope assembly in a human cell-free system.
2001,
Pubmed
,
Xenbase Zhang,
Role of importin-beta in the control of nuclear envelope assembly by Ran.
2002,
Pubmed
,
Xenbase Zhang,
Concentration of Ran on chromatin induces decondensation, nuclear envelope formation and nuclear pore complex assembly.
2002,
Pubmed
,
Xenbase Zhang,
Chromatin-independent nuclear envelope assembly induced by Ran GTPase in Xenopus egg extracts.
2000,
Pubmed
,
Xenbase
