Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
???displayArticle.abstract???
One important component of the cell-cell communication that occurs during regenerative patterning is bioelectrical signaling. In particular, the regeneration of the tail in Xenopus laevis tadpoles both requires, and can be initiated at non-regenerative stages by, specific regulation of bioelectrical signaling (alteration in resting membrane potential and a subsequent change in sodium content of blastemal cells). Although standing gradients of transmembrane voltage and ion concentration can provide positional guidance and other morphogenetic cues, these biophysical parameters must be transduced into transcriptional responses within cells. A number of mechanisms have been described for linking slow voltage changes to gene expression, but recent data on the importance of epigenetic regulation for regeneration suggest a novel hypothesis: that sodium/butyrate transporters link ion flows to influx of small molecules needed to modify chromatin state. Here, we briefly review the data on bioelectricity in tadpoletail regeneration, present a technique for convenient alteration of transmembrane potential in vivo that does not require transgenes, show augmentation of regeneration in vivo by manipulation of voltage, and present new data in the Xenopus tail consistent with the hypothesis that the monocarboxlyate transporter SLC5A8 may link regeneration-relevant epigenetic modification with upstream changes in ion content.
Adams,
Early, H+-V-ATPase-dependent proton flux is necessary for consistent left-right patterning of non-mammalian vertebrates.
2006, Pubmed,
Xenbase
Adams,
Early, H+-V-ATPase-dependent proton flux is necessary for consistent left-right patterning of non-mammalian vertebrates.
2006,
Pubmed
,
Xenbase Adams,
H+ pump-dependent changes in membrane voltage are an early mechanism necessary and sufficient to induce Xenopus tail regeneration.
2007,
Pubmed
,
Xenbase Anderson,
Loss of Dnmt1 catalytic activity reveals multiple roles for DNA methylation during pancreas development and regeneration.
2009,
Pubmed Arcangeli,
Expression and role of hERG channels in cancer cells.
2005,
Pubmed Arosio,
Simultaneous intracellular chloride and pH measurements using a GFP-based sensor.
2010,
Pubmed Bao,
Epigenetic reversion of post-implantation epiblast to pluripotent embryonic stem cells.
2009,
Pubmed Barrero,
Regenerating the epigenome.
2011,
Pubmed Beane,
A chemical genetics approach reveals H,K-ATPase-mediated membrane voltage is required for planarian head regeneration.
2011,
Pubmed Becchetti,
Ion channels and transporters in cancer. 1. Ion channels and cell proliferation in cancer.
2011,
Pubmed Beck,
The role of BMP signaling in outgrowth and patterning of the Xenopus tail bud.
2001,
Pubmed
,
Xenbase Beck,
An amphibian with ambition: a new role for Xenopus in the 21st century.
2001,
Pubmed
,
Xenbase Beck,
Notch is required for outgrowth of the Xenopus tail bud.
2002,
Pubmed
,
Xenbase Beck,
A developmental pathway controlling outgrowth of the Xenopus tail bud.
1999,
Pubmed
,
Xenbase Beck,
Molecular pathways needed for regeneration of spinal cord and muscle in a vertebrate.
2003,
Pubmed
,
Xenbase Beck,
Beyond early development: Xenopus as an emerging model for the study of regenerative mechanisms.
2009,
Pubmed
,
Xenbase Beck,
Temporal requirement for bone morphogenetic proteins in regeneration of the tail and limb of Xenopus tadpoles.
2006,
Pubmed
,
Xenbase Blackiston,
Transmembrane potential of GlyCl-expressing instructor cells induces a neoplastic-like conversion of melanocytes via a serotonergic pathway.
2011,
Pubmed
,
Xenbase Blackiston,
Bioelectric controls of cell proliferation: ion channels, membrane voltage and the cell cycle.
2009,
Pubmed Brower,
Epigenetics: Unravelling the cancer code.
2011,
Pubmed Bubenik,
Trophic responses to trauma in growing antlers.
1965,
Pubmed Burr,
Bio-Electric Correlates of Development in Amblystoma.
1937,
Pubmed Carneiro,
Histone deacetylase activity is necessary for left-right patterning during vertebrate development.
2011,
Pubmed
,
Xenbase Contreras,
Early requirement of Hyaluronan for tail regeneration in Xenopus tadpoles.
2009,
Pubmed
,
Xenbase Costa,
Novel gene expression domains reveal early patterning of the Xenopus endoderm.
2003,
Pubmed
,
Xenbase Deisseroth,
Excitation-neurogenesis coupling in adult neural stem/progenitor cells.
2004,
Pubmed DeSimone,
The Xenopus embryo as a model system for studies of cell migration.
2005,
Pubmed
,
Xenbase Dvash,
Epigenetic regulation of X-inactivation in human embryonic stem cells.
2009,
Pubmed Epps,
Characterization of the steady-state and dynamic fluorescence properties of the potential-sensitive dye bis-(1,3-dibutylbarbituric acid)trimethine oxonol (Dibac4(3)) in model systems and cells.
1994,
Pubmed Fagot,
Antiproliferative effects of sodium butyrate in adriamycin-sensitive and -resistant human cancer cell lines.
1994,
Pubmed Franchini,
Tail regenerative capacity and iNOS immunolocalization in Xenopus laevis tadpoles.
2011,
Pubmed
,
Xenbase Fraser,
Voltage-gated sodium channel expression and potentiation of human breast cancer metastasis.
2005,
Pubmed Fukazawa,
Suppression of the immune response potentiates tadpole tail regeneration during the refractory period.
2009,
Pubmed
,
Xenbase Fukumoto,
Serotonin transporter function is an early step in left-right patterning in chick and frog embryos.
2005,
Pubmed
,
Xenbase Gargioli,
Cell lineage tracing during Xenopus tail regeneration.
2004,
Pubmed
,
Xenbase Hague,
Sodium butyrate induces apoptosis in human colonic tumour cell lines in a p53-independent pathway: implications for the possible role of dietary fibre in the prevention of large-bowel cancer.
1993,
Pubmed Hajkova,
Chromatin dynamics during epigenetic reprogramming in the mouse germ line.
2008,
Pubmed Harland,
In situ hybridization: an improved whole-mount method for Xenopus embryos.
1991,
Pubmed
,
Xenbase Hechavarria,
BioDome regenerative sleeve for biochemical and biophysical stimulation of tissue regeneration.
2010,
Pubmed Ho,
TGF-beta signaling is required for multiple processes during Xenopus tail regeneration.
2008,
Pubmed
,
Xenbase House,
Voltage-gated Na+ channel SCN5A is a key regulator of a gene transcriptional network that controls colon cancer invasion.
2010,
Pubmed Illingworth,
Trapped fingers and amputated finger tips in children.
1974,
Pubmed Jänicke,
Caspase-3 is required for DNA fragmentation and morphological changes associated with apoptosis.
1998,
Pubmed Jenkins,
Reduction of the current of injury leaving the amputation inhibits limb regeneration in the red spotted newt.
1996,
Pubmed Koide,
Xenopus as a model system to study transcriptional regulatory networks.
2005,
Pubmed
,
Xenbase Kragl,
Cells keep a memory of their tissue origin during axolotl limb regeneration.
2009,
Pubmed Levin,
Large-scale biophysics: ion flows and regeneration.
2007,
Pubmed Levin,
Asymmetries in H+/K+-ATPase and cell membrane potentials comprise a very early step in left-right patterning.
2002,
Pubmed
,
Xenbase Levin,
Bioelectric mechanisms in regeneration: Unique aspects and future perspectives.
2009,
Pubmed Li,
SLC5A8, a sodium transporter, is a tumor suppressor gene silenced by methylation in human colon aberrant crypt foci and cancers.
2003,
Pubmed Lin,
Regeneration of neural crest derivatives in the Xenopus tadpole tail.
2007,
Pubmed
,
Xenbase Lynch,
Native glycine receptor subtypes and their physiological roles.
2009,
Pubmed Mandal,
Bcl-2 deregulation leads to inhibition of sodium butyrate-induced apoptosis in human colorectal carcinoma cells.
1997,
Pubmed MARSH,
Electrical control of morphogenesis in regenerating Dugesia tigrina. I. Relation of axial polarity to field strength.
1952,
Pubmed McCaig,
Controlling cell behavior electrically: current views and future potential.
2005,
Pubmed McCaig,
Electrical dimensions in cell science.
2009,
Pubmed Miyauchi,
Functional identification of SLC5A8, a tumor suppressor down-regulated in colon cancer, as a Na(+)-coupled transporter for short-chain fatty acids.
2004,
Pubmed
,
Xenbase Mochii,
Tail regeneration in the Xenopus tadpole.
2007,
Pubmed
,
Xenbase Mondia,
Long-distance signals are required for morphogenesis of the regenerating Xenopus tadpole tail, as shown by femtosecond-laser ablation.
2011,
Pubmed
,
Xenbase Murata,
Phosphoinositide phosphatase activity coupled to an intrinsic voltage sensor.
2005,
Pubmed
,
Xenbase Nogi,
Characterization of innexin gene expression and functional roles of gap-junctional communication in planarian regeneration.
2005,
Pubmed Nuccitelli,
On electrical currents in development.
1986,
Pubmed Nuccitelli,
Endogenous electric fields in embryos during development, regeneration and wound healing.
2003,
Pubmed Okamura,
Voltage-sensing phosphatase: its molecular relationship with PTEN.
2011,
Pubmed Olivotto,
Electric fields at the plasma membrane level: a neglected element in the mechanisms of cell signalling.
1996,
Pubmed Oviedo,
Live Imaging of Planarian Membrane Potential Using DiBAC4(3).
2008,
Pubmed Oviedo,
Long-range neural and gap junction protein-mediated cues control polarity during planarian regeneration.
2010,
Pubmed Ozkucur,
Ion imaging during axolotl tail regeneration in vivo.
2010,
Pubmed Pai,
Transmembrane voltage potential controls embryonic eye patterning in Xenopus laevis.
2012,
Pubmed
,
Xenbase Reid,
Electric currents in Xenopus tadpole tail regeneration.
2009,
Pubmed
,
Xenbase Rinkevich,
Germ-layer and lineage-restricted stem/progenitors regenerate the mouse digit tip.
2011,
Pubmed Roepke,
Targeted deletion of Kcne2 causes gastritis cystica profunda and gastric neoplasia.
2010,
Pubmed Sasaki,
Dynamic regulation of neuronal NO synthase transcription by calcium influx through a CREB family transcription factor-dependent mechanism.
2000,
Pubmed Shan,
Ivermectin, an unconventional agonist of the glycine receptor chloride channel.
2001,
Pubmed Shi,
Three-dimensional gradients of voltage during development of the nervous system as invisible coordinates for the establishment of embryonic pattern.
1995,
Pubmed Siu,
Activity-dependent hepatocyte growth factor expression and its role in organogenesis and cancer growth suppression.
2004,
Pubmed Stewart,
A histone demethylase is necessary for regeneration in zebrafish.
2009,
Pubmed Straka,
Xenopus laevis: an ideal experimental model for studying the developmental dynamics of neural network assembly and sensory-motor computations.
2012,
Pubmed
,
Xenbase Sundelacruz,
Role of membrane potential in the regulation of cell proliferation and differentiation.
2009,
Pubmed Taylor,
Changes in phenotypic expression in embryonic and adult cells treated with 5-azacytidine.
1982,
Pubmed Tseng,
Apoptosis is required during early stages of tail regeneration in Xenopus laevis.
2007,
Pubmed
,
Xenbase Tseng,
Induction of vertebrate regeneration by a transient sodium current.
2010,
Pubmed
,
Xenbase Tseng,
Tail regeneration in Xenopus laevis as a model for understanding tissue repair.
2008,
Pubmed
,
Xenbase Tseng,
HDAC activity is required during Xenopus tail regeneration.
2011,
Pubmed
,
Xenbase Vandenberg,
V-ATPase-dependent ectodermal voltage and pH regionalization are required for craniofacial morphogenesis.
2011,
Pubmed
,
Xenbase Vergara,
Retinal regeneration in the Xenopus laevis tadpole: a new model system.
2009,
Pubmed
,
Xenbase Waseem,
Genetically encoded Cl-Sensor as a tool for monitoring of Cl-dependent processes in small neuronal compartments.
2010,
Pubmed Yakushiji,
Correlation between Shh expression and DNA methylation status of the limb-specific Shh enhancer region during limb regeneration in amphibians.
2007,
Pubmed
,
Xenbase
