Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Proc Biol Sci
2012 Dec 07;2791748:4795-802. doi: 10.1098/rspb.2012.1945.
Show Gene links
Show Anatomy links
The epithelial sodium channel in the Australian lungfish, Neoceratodus forsteri (Osteichthyes: Dipnoi).
Uchiyama M, Maejima S, Yoshie S, Kubo Y, Konno N, Joss JM.
???displayArticle.abstract???
Epithelial sodium channel (ENaC) is a Na(+)-selective, aldosterone-stimulated ion channel involved in sodium transport homeostasis. ENaC is rate-limiting for Na(+) absorption in the epithelia of osmoregulatory organs of tetrapods. Although the ENaC/degenerin gene family is proposed to be present in metazoans, no orthologues or paralogues for ENaC have been found in the genome databases of teleosts. We studied full-length cDNA cloning and tissue distributions of ENaCα, β and γ subunits in the Australian lungfish, Neoceratodus forsteri, which is the closest living relative of tetrapods. Neoceratodus ENaC (nENaC) comprised three subunits: nENaCα, β and γ proteins. The nENaCα, β and γ subunits are closely related to amphibian ENaCα, β and γ subunits, respectively. Three ENaC subunit mRNAs were highly expressed in the gills, kidney and rectum. Amiloride-sensitive sodium current was recorded from Xenopus oocytes injected with the nENaCαβγ subunit complementary RNAs under a two-electrode voltage clamp. nENaCα immunoreactivity was observed in the apical cell membrane of the gills, kidney and rectum. Thus, nENaC may play a role in regulating sodium transport of the lungfish, which has a renin-angiotensin-aldosterone system. This is interesting because there may have been an ENaC sodium absorption system controlled by aldosterone before the conquest of land by vertebrates.
Alvarez de la Rosa,
Structure and regulation of amiloride-sensitive sodium channels.
2000, Pubmed
Alvarez de la Rosa,
Structure and regulation of amiloride-sensitive sodium channels.
2000,
Pubmed Benos,
Functional domains within the degenerin/epithelial sodium channel (Deg/ENaC) superfamily of ion channels.
1999,
Pubmed Brinkmann,
Nuclear protein-coding genes support lungfish and not the coelacanth as the closest living relatives of land vertebrates.
2004,
Pubmed Bruns,
Epithelial Na+ channels are fully activated by furin- and prostasin-dependent release of an inhibitory peptide from the gamma-subunit.
2007,
Pubmed
,
Xenbase Canessa,
Amiloride-sensitive epithelial Na+ channel is made of three homologous subunits.
1994,
Pubmed
,
Xenbase Fujiwara,
Voltage- and [ATP]-dependent gating of the P2X(2) ATP receptor channel.
2009,
Pubmed
,
Xenbase Fyfe,
Subunit composition determines the single channel kinetics of the epithelial sodium channel.
1998,
Pubmed
,
Xenbase Garty,
Epithelial sodium channels: function, structure, and regulation.
1997,
Pubmed Giraldez,
The epithelial sodium channel δ-subunit: new notes for an old song.
2012,
Pubmed Goldstein,
Cloning and induction by low NaCl intake of avian intestine Na+ channel subunits.
1997,
Pubmed
,
Xenbase Hughey,
Epithelial sodium channels are activated by furin-dependent proteolysis.
2004,
Pubmed
,
Xenbase Jasti,
Structure of acid-sensing ion channel 1 at 1.9 A resolution and low pH.
2007,
Pubmed Kashlan,
ENaC structure and function in the wake of a resolved structure of a family member.
2011,
Pubmed Kellenberger,
Epithelial sodium channel/degenerin family of ion channels: a variety of functions for a shared structure.
2002,
Pubmed Konno,
Vasotocin/V2-type receptor/aquaporin axis exists in African lungfish kidney but is functional only in terrestrial condition.
2010,
Pubmed
,
Xenbase Konno,
Immunolocalization and mRNA expression of the epithelial Na+ channel alpha-subunit in the kidney and urinary bladder of the marine toad, Bufo marinus, under hyperosmotic conditions.
2007,
Pubmed
,
Xenbase Mamenko,
Angiotensin II increases activity of the epithelial Na+ channel (ENaC) in distal nephron additively to aldosterone.
2012,
Pubmed Meyer,
From 2R to 3R: evidence for a fish-specific genome duplication (FSGD).
2005,
Pubmed Puoti,
The highly selective low-conductance epithelial Na channel of Xenopus laevis A6 kidney cells.
1995,
Pubmed
,
Xenbase Snyder,
Relative contribution of Nedd4 and Nedd4-2 to ENaC regulation in epithelia determined by RNA interference.
2004,
Pubmed Stewart,
Atomic force microscopy reveals the architecture of the epithelial sodium channel (ENaC).
2011,
Pubmed
,
Xenbase Studer,
Evolution of the epithelial sodium channel and the sodium pump as limiting factors of aldosterone action on sodium transport.
2011,
Pubmed Sturla,
Mitochondria-rich cells in gills and skin of an African lungfish, Protopterus annectens.
2001,
Pubmed Uchiyama,
Ontogeny of ENaC expression in the gills and the kidneys of the Japanese black salamander (Hynobius nigrescens Stejneger).
2011,
Pubmed Uchiyama,
Immunohistological classification of ionocytes in the external gills of larval Japanese black salamander, Hynobius nigrescens Stejneger.
2011,
Pubmed Waldmann,
Molecular cloning and functional expression of a novel amiloride-sensitive Na+ channel.
1995,
Pubmed
,
Xenbase
