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Munoz C, Almilaji A, Setiawan I, Föller M, Lang F.
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The inward rectifier K⁺ channel Kir2.1 contributes to the maintenance of the resting cell membrane potential in excitable cells. Loss of function mutations of KCNJ2 encoding Kir2.1 result in Andersen-Tawil syndrome, a disorder characterized by periodic paralysis, cardiac arrhythmia, and dysmorphic features. The ubiquitously expressed protein kinase B (PKB/Akt) activates the phosphatidylinositol-3-phosphate-5-kinase PIKfyve, which in turn regulates a variety of carriers and channels. The present study explored whether PKB/PIKfve contributes to the regulation of Kir2.1. To this end, cRNA encoding Kir2.1 was injected into Xenopus oocytes with and without additional injection of cRNA encoding wild type PKB (PKB), constitutively active (T308D,S473D)PKB or inactive (T308A,S473A)PKB. Kir2.1 activity was determined by two-electrode voltage-clamp. As a result, PKB and (T308D,S473D)PKB, but not (T308A,S473A)PKB, significantly increased Kir2.1-mediated currents. The effect of PKB was mimicked by coexpression of PIKfyve but not of (S318A)Pikfyve lacking the PKB phosphorylation site. The decay of Kir2.1-mediated currents after inhibition of channel insertion into the cell membrane by brefeldin A (5 μM) was similar in oocytes expressing Kir2.1 + PKB or Kir2.1 + PIKfyve to those expressing Kir2.1 alone, suggesting that PKB and PIKfyve influence channel insertion into rather than channel retrieval from the cell membrane. In conclusion, PKB and PIKfyve are novel regulators of Kir2.1.
Alesutan,
Regulation of the glutamate transporter EAAT4 by PIKfyve.
2010, Pubmed,
Xenbase
Alesutan,
Regulation of the glutamate transporter EAAT4 by PIKfyve.
2010,
Pubmed
,
Xenbase Andersen,
Intermittent muscular weakness, extrasystoles, and multiple developmental anomalies. A new syndrome?
1971,
Pubmed Becker,
Hydrophobic bile salts induce hepatocyte shrinkage via NADPH oxidase activation.
2007,
Pubmed Berwick,
Protein kinase B phosphorylation of PIKfyve regulates the trafficking of GLUT4 vesicles.
2004,
Pubmed Bhandaru,
Decreased bone density and increased phosphaturia in gene-targeted mice lacking functional serum- and glucocorticoid-inducible kinase 3.
2011,
Pubmed
,
Xenbase Boehmer,
Post-translational regulation of EAAT2 function by co-expressed ubiquitin ligase Nedd4-2 is impacted by SGK kinases.
2006,
Pubmed
,
Xenbase Boehmer,
Regulation of the excitatory amino acid transporter EAAT5 by the serum and glucocorticoid dependent kinases SGK1 and SGK3.
2005,
Pubmed
,
Xenbase Böhmer,
The serum and glucocorticoid inducible kinases SGK1-3 stimulate the neutral amino acid transporter SLC6A19.
2010,
Pubmed
,
Xenbase Boiteux,
Design of glycolysis.
1981,
Pubmed Bortner,
The role of apoptotic volume decrease and ionic homeostasis in the activation and repression of apoptosis.
2004,
Pubmed Bravo,
Amphibian oocyte maturation and protein synthesis: related inhibition by cyclic AMP, theophylline, and papaverine.
1978,
Pubmed
,
Xenbase Collins,
Differential sensitivity of inward rectifier K+ channels to metabolic inhibitors.
2002,
Pubmed
,
Xenbase Dart,
Targeting of an A kinase-anchoring protein, AKAP79, to an inwardly rectifying potassium channel, Kir2.1.
2001,
Pubmed de Lartigue,
PIKfyve regulation of endosome-linked pathways.
2009,
Pubmed Dërmaku-Sopjani,
Downregulation of NaPi-IIa and NaPi-IIb Na-coupled phosphate transporters by coexpression of Klotho.
2011,
Pubmed
,
Xenbase Diaz,
Selective inhibition of inward rectifier K+ channels (Kir2.1 or Kir2.2) abolishes protection by ischemic preconditioning in rabbit ventricular cardiomyocytes.
2004,
Pubmed Dieter,
Regulation of glucose transporter SGLT1 by ubiquitin ligase Nedd4-2 and kinases SGK1, SGK3, and PKB.
2004,
Pubmed
,
Xenbase Donaldson,
PIP2 binding residues of Kir2.1 are common targets of mutations causing Andersen syndrome.
2003,
Pubmed Eckey,
Modulation of human ether a gogo related channels by CASQ2 contributes to etiology of catecholaminergic polymorphic ventricular tachycardia (CPVT).
2010,
Pubmed
,
Xenbase Föller,
PGE2-induced apoptotic cell death in K562 human leukaemia cells.
2006,
Pubmed Gehring,
PIKfyve upregulates CFTR activity.
2009,
Pubmed
,
Xenbase Gehring,
Regulation of the glutamate transporter EAAT2 by PIKfyve.
2009,
Pubmed
,
Xenbase Henry,
Protein kinase C inhibition of cloned inward rectifier (HRK1/KIR2.3) K+ channels expressed in Xenopus oocytes.
1996,
Pubmed
,
Xenbase Hibino,
Inwardly rectifying potassium channels: their structure, function, and physiological roles.
2010,
Pubmed Hill,
Regulation of PIKfyve phosphorylation by insulin and osmotic stress.
2010,
Pubmed Hosseinzadeh,
Regulation of the glutamate transporters by JAK2.
2011,
Pubmed
,
Xenbase Ikonomov,
PIKfyve controls fluid phase endocytosis but not recycling/degradation of endocytosed receptors or sorting of procathepsin D by regulating multivesicular body morphogenesis.
2003,
Pubmed Ikonomov,
Functional dissection of lipid and protein kinase signals of PIKfyve reveals the role of PtdIns 3,5-P2 production for endomembrane integrity.
2002,
Pubmed Ikonomov,
Localized PtdIns 3,5-P2 synthesis to regulate early endosome dynamics and fusion.
2006,
Pubmed Ikonomov,
Mammalian cell morphology and endocytic membrane homeostasis require enzymatically active phosphoinositide 5-kinase PIKfyve.
2001,
Pubmed Ikonomov,
PIKfyve-ArPIKfyve-Sac3 core complex: contact sites and their consequence for Sac3 phosphatase activity and endocytic membrane homeostasis.
2009,
Pubmed Jones,
Role of the small GTPase Rho in modulation of the inwardly rectifying potassium channel Kir2.1.
2003,
Pubmed Kirkbride,
Regulation of late endosomal/lysosomal maturation and trafficking by cortactin affects Golgi morphology.
2012,
Pubmed Klaus,
Up-regulation of hypertonicity-activated myo-inositol transporter SMIT1 by the cell volume-sensitive protein kinase SGK1.
2008,
Pubmed
,
Xenbase Klaus,
PIKfyve-dependent regulation of the Cl- channel ClC-2.
2009,
Pubmed
,
Xenbase Klausner,
Brefeldin A: insights into the control of membrane traffic and organelle structure.
1992,
Pubmed Lang,
Potassium channels in renal epithelial transport regulation.
1992,
Pubmed Lang,
Electrophysiology of sodium-coupled transport in proximal renal tubules.
1986,
Pubmed Lang,
Functional significance of cell volume regulatory mechanisms.
1998,
Pubmed Leonoudakis,
A multiprotein trafficking complex composed of SAP97, CASK, Veli, and Mint1 is associated with inward rectifier Kir2 potassium channels.
2004,
Pubmed Leyland,
An alternatively spliced isoform of PSD-93/chapsyn 110 binds to the inwardly rectifying potassium channel, Kir2.1.
2004,
Pubmed Liu,
Direct activation of an inwardly rectifying potassium channel by arachidonic acid.
2001,
Pubmed Liu,
Comparison of cloned Kir2 channels with native inward rectifier K+ channels from guinea-pig cardiomyocytes.
2001,
Pubmed
,
Xenbase Lodge,
Alterations in Ito1, IKr and Ik1 density in the BIO TO-2 strain of syrian myopathic hamsters.
1997,
Pubmed Lopatin,
Inward rectifiers in the heart: an update on I(K1).
2001,
Pubmed Lupescu,
Inhibition of Na+/H+ exchanger activity by parvovirus B19 protein NS1.
2009,
Pubmed Mohamed,
Functional analysis of a novel I71N mutation in the GJB2 gene among Southern Egyptians causing autosomal recessive hearing loss.
2010,
Pubmed
,
Xenbase Morris,
Measurement of autotaxin/lysophospholipase D activity.
2007,
Pubmed Nehring,
Neuronal inwardly rectifying K(+) channels differentially couple to PDZ proteins of the PSD-95/SAP90 family.
2000,
Pubmed O'Connor,
Inhibition of oocyte maturation by theophylline: possible mechanism of action.
1976,
Pubmed
,
Xenbase Palmada,
The serine/threonine kinases SGK1, 3 and PKB stimulate the amino acid transporter ASCT2.
2005,
Pubmed
,
Xenbase Plaster,
Mutations in Kir2.1 cause the developmental and episodic electrical phenotypes of Andersen's syndrome.
2001,
Pubmed
,
Xenbase Preisig-Müller,
Heteromerization of Kir2.x potassium channels contributes to the phenotype of Andersen's syndrome.
2002,
Pubmed
,
Xenbase Rexhepaj,
Stimulation of electrogenic glucose transport by glycogen synthase kinase 3.
2010,
Pubmed
,
Xenbase Rohács,
Distinct specificities of inwardly rectifying K(+) channels for phosphoinositides.
1999,
Pubmed
,
Xenbase Romanenko,
Cholesterol sensitivity and lipid raft targeting of Kir2.1 channels.
2004,
Pubmed Rusten,
Fab1 phosphatidylinositol 3-phosphate 5-kinase controls trafficking but not silencing of endocytosed receptors.
2006,
Pubmed Rutherford,
The mammalian phosphatidylinositol 3-phosphate 5-kinase (PIKfyve) regulates endosome-to-TGN retrograde transport.
2006,
Pubmed Sampson,
Direct interaction between the actin-binding protein filamin-A and the inwardly rectifying potassium channel, Kir2.1.
2003,
Pubmed Sansone,
Andersen's syndrome: a distinct periodic paralysis.
1997,
Pubmed Sbrissa,
PIKfyve, a mammalian ortholog of yeast Fab1p lipid kinase, synthesizes 5-phosphoinositides. Effect of insulin.
1999,
Pubmed Sbrissa,
Phosphatidylinositol 5-phosphate biosynthesis is linked to PIKfyve and is involved in osmotic response pathway in mammalian cells.
2002,
Pubmed Sbrissa,
Role for a novel signaling intermediate, phosphatidylinositol 5-phosphate, in insulin-regulated F-actin stress fiber breakdown and GLUT4 translocation.
2004,
Pubmed Schneider,
Suicidal erythrocyte death following cellular K+ loss.
2007,
Pubmed Seebohm,
Regulation of endocytic recycling of KCNQ1/KCNE1 potassium channels.
2007,
Pubmed
,
Xenbase Shimizu,
Inhibition of hypertonicity-induced cation channels sensitizes HeLa cells to shrinkage-induced apoptosis.
2006,
Pubmed Shojaiefard,
Stimulation of the creatine transporter SLC6A8 by the protein kinases SGK1 and SGK3.
2005,
Pubmed
,
Xenbase Shojaiefard,
Regulation of the Na(+), glucose cotransporter by PIKfyve and the serum and glucocorticoid inducible kinase SGK1.
2007,
Pubmed
,
Xenbase Soom,
Multiple PIP2 binding sites in Kir2.1 inwardly rectifying potassium channels.
2001,
Pubmed
,
Xenbase Sopjani,
Regulation of the Ca(2+) channel TRPV6 by the kinases SGK1, PKB/Akt, and PIKfyve.
2010,
Pubmed
,
Xenbase Strutz-Seebohm,
Structural basis of slow activation gating in the cardiac I Ks channel complex.
2011,
Pubmed
,
Xenbase Strutz-Seebohm,
PIKfyve in the SGK1 mediated regulation of the creatine transporter SLC6A8.
2007,
Pubmed
,
Xenbase Tawil,
Andersen's syndrome: potassium-sensitive periodic paralysis, ventricular ectopy, and dysmorphic features.
1994,
Pubmed Tristani-Firouzi,
Kir 2.1 channelopathies: the Andersen-Tawil syndrome.
2010,
Pubmed Tsuruta,
PIKfyve regulates CaV1.2 degradation and prevents excitotoxic cell death.
2009,
Pubmed Ureche,
Novel insights into the structural basis of pH-sensitivity in inward rectifier K+ channels Kir2.3.
2008,
Pubmed
,
Xenbase Vicente,
The systemic inflammatory response is involved in the regulation of K(+) channel expression in brain via TNF-alpha-dependent and -independent pathways.
2004,
Pubmed Watson,
Bridging the GAP between insulin signaling and GLUT4 translocation.
2006,
Pubmed Welsh,
Role of protein kinase B in insulin-regulated glucose uptake.
2005,
Pubmed Wischmeyer,
Acute suppression of inwardly rectifying Kir2.1 channels by direct tyrosine kinase phosphorylation.
1998,
Pubmed
,
Xenbase Xia,
A Kir2.1 gain-of-function mutation underlies familial atrial fibrillation.
2005,
Pubmed Xiao,
Localization of PIP2 activation gate in inward rectifier K+ channels.
2003,
Pubmed
,
Xenbase Zheng,
ADP-ribosylation factor6 regulates both [3H]-noradrenaline and [14C]-glutamate exocytosis through phosphatidylinositol 4,5-bisphosphate.
2004,
Pubmed Zitron,
Human cardiac inwardly rectifying current IKir2.2 is upregulated by activation of protein kinase A.
2004,
Pubmed
,
Xenbase
