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J Biol Chem
2013 Jan 11;2882:1279-85. doi: 10.1074/jbc.M112.413989.
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In vivo activation of azipropofol prolongs anesthesia and reveals synaptic targets.
Weiser BP, Kelz MB, Eckenhoff RG.
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General anesthetic photolabels have been instrumental in discovering and confirming protein binding partners and binding sites of these promiscuous ligands. We report the in vivo photoactivation of meta-azipropofol, a potent analog of propofol, in Xenopus laevis tadpoles. Covalent adduction of meta-azipropofol in vivo prolongs the primary pharmacologic effect of general anesthetics in a behavioral phenotype we termed "optoanesthesia." Coupling this behavior with a tritiated probe, we performed unbiased, time-resolved gel proteomics to identify neuronal targets of meta-azipropofol in vivo. We have identified synaptic binding partners, such as synaptosomal-associated protein 25, as well as voltage-dependent anion channels as potential facilitators of the general anesthetic state. Pairing behavioral phenotypes elicited by the activation of efficacious photolabels in vivo with time-resolved proteomics provides a novel approach to investigate molecular mechanisms of general anesthetics.
Blackmer,
G protein betagamma directly regulates SNARE protein fusion machinery for secretory granule exocytosis.
2005, Pubmed
Blackmer,
G protein betagamma directly regulates SNARE protein fusion machinery for secretory granule exocytosis.
2005,
Pubmed Bunai,
Effectiveness and limitation of two-dimensional gel electrophoresis in bacterial membrane protein proteomics and perspectives.
2005,
Pubmed Bureau,
Isolation and cloning of a voltage-dependent anion channel-like Mr 36,000 polypeptide from mammalian brain.
1992,
Pubmed Chen,
Neurosteroid analog photolabeling of a site in the third transmembrane domain of the β3 subunit of the GABA(A) receptor.
2012,
Pubmed Chen,
A neurosteroid analogue photolabeling reagent labels the colchicine-binding site on tubulin: a mass spectrometric analysis.
2012,
Pubmed Cheng,
VDAC2 inhibits BAK activation and mitochondrial apoptosis.
2003,
Pubmed Condliffe,
Endogenous SNAP-25 regulates native voltage-gated calcium channels in glutamatergic neurons.
2010,
Pubmed Cui,
NMR structure and dynamics of a designed water-soluble transmembrane domain of nicotinic acetylcholine receptor.
2012,
Pubmed Darbandi-Tonkabon,
Photoaffinity labeling with a neuroactive steroid analogue. 6-azi-pregnanolone labels voltage-dependent anion channel-1 in rat brain.
2003,
Pubmed
,
Xenbase Darbandi-Tonkabon,
Neuroactive steroid interactions with voltage-dependent anion channels: lack of relationship to GABA(A) receptor modulation and anesthesia.
2004,
Pubmed Das,
Identification of a general anesthetic binding site in the diacylglycerol-binding domain of protein kinase Cdelta.
2004,
Pubmed Downes,
Contrasting effects of anesthetics in tadpole bioassays.
1996,
Pubmed Eckenhoff,
Halothane binding to soluble proteins determined by photoaffinity labeling.
1993,
Pubmed Eckenhoff,
Azi-isoflurane, a Photolabel Analog of the Commonly Used Inhaled General Anesthetic Isoflurane.
2010,
Pubmed Friedman,
A conserved behavioral state barrier impedes transitions between anesthetic-induced unconsciousness and wakefulness: evidence for neural inertia.
2010,
Pubmed Garbelli,
Heterogeneous expression of SNAP-25 in rat and human brain.
2008,
Pubmed Gerachshenko,
Gbetagamma acts at the C terminus of SNAP-25 to mediate presynaptic inhibition.
2005,
Pubmed Hall,
m-Azipropofol (AziPm) a photoactive analogue of the intravenous general anesthetic propofol.
2010,
Pubmed
,
Xenbase Herlitze,
Modulation of Ca2+ channels by G-protein beta gamma subunits.
1996,
Pubmed Herring,
Isoflurane inhibits the neurotransmitter release machinery.
2009,
Pubmed Herring,
Etomidate and propofol inhibit the neurotransmitter release machinery at different sites.
2011,
Pubmed Husain,
2-(3-Methyl-3H-diaziren-3-yl)ethyl 1-(1-phenylethyl)-1H-imidazole-5-carboxylate: a derivative of the stereoselective general anesthetic etomidate for photolabeling ligand-gated ion channels.
2003,
Pubmed
,
Xenbase Ikeda,
Voltage-dependent modulation of N-type calcium channels by G-protein beta gamma subunits.
1996,
Pubmed Issaq,
Two-dimensional polyacrylamide gel electrophoresis (2D-PAGE): advances and perspectives.
2008,
Pubmed Jurd,
General anesthetic actions in vivo strongly attenuated by a point mutation in the GABA(A) receptor beta3 subunit.
2003,
Pubmed Krasowski,
General anesthetic potencies of a series of propofol analogs correlate with potency for potentiation of gamma-aminobutyric acid (GABA) current at the GABA(A) receptor but not with lipid solubility.
2001,
Pubmed
,
Xenbase Li,
Identification of a GABAA receptor anesthetic binding site at subunit interfaces by photolabeling with an etomidate analog.
2006,
Pubmed Nagele,
Volatile anesthetics bind rat synaptic snare proteins.
2005,
Pubmed Nguyen,
Behavior and cellular evidence for propofol-induced hypnosis involving brain glycine receptors.
2009,
Pubmed Stewart,
p-(4-Azipentyl)propofol: a potent photoreactive general anesthetic derivative of propofol.
2011,
Pubmed
,
Xenbase van Swinderen,
A neomorphic syntaxin mutation blocks volatile-anesthetic action in Caenorhabditis elegans.
1999,
Pubmed Verderio,
SNAP-25 modulation of calcium dynamics underlies differences in GABAergic and glutamatergic responsiveness to depolarization.
2004,
Pubmed Wiser,
The voltage sensitive Lc-type Ca2+ channel is functionally coupled to the exocytotic machinery.
1999,
Pubmed
,
Xenbase Xi,
Inhalational anesthetic-binding proteins in rat neuronal membranes.
2004,
Pubmed Yuki,
Isoflurane binds and stabilizes a closed conformation of the leukocyte function-associated antigen-1.
2012,
Pubmed Zhong,
Photo-activated azi-etomidate, a general anesthetic photolabel, irreversibly enhances gating and desensitization of gamma-aminobutyric acid type A receptors.
2008,
Pubmed
,
Xenbase Ziebell,
Identification of binding sites in the nicotinic acetylcholine receptor for [3H]azietomidate, a photoactivatable general anesthetic.
2004,
Pubmed
