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Early interspecies nuclear transfer (iNT) experiments suggested that a foreign nucleus may become permanently damaged after a few rounds of cell division in the cytoplasm of another species. That is, in some distant species combinations, nucleocytoplasmic hybrid (cybrid) blastula nuclei can no longer support development, even if they are back-transferred into their own kind of eggcytoplasm. We monitored foreign DNA amplification and RNA production by quantitative PCR (qPCR) and RT-qPCR in interorder amphibian hybrids and cybrids formed by the transfer of newt (Pleurodeles waltl) embryonic nuclei into intact and enucleated frog (Xenopus laevis) eggs. We found a dramatic reduction in the expansion of foreign DNA and cell numbers in developing cybrid embryos that correlated with reduced gene transcription. Interestingly, expansion in cell numbers was rescued by the recipient species (Xenopus) maternal genome in iNT hybrids, but it did not improve P. waltl DNA expansion or gene transcription. Also, foreign gene transcripts, normalized to DNA copy numbers, were mostly normal in both iNT hybrids and cybrids. Thus, incomplete foreign DNA replication and/or chromosome segregation during cell division may be the major form of nuclear damage occurring as a result of nuclear replication in a foreign cytoplasmic environment. It also shows that the mechanisms of embryonic gene transcription are highly conserved across amphibians and may not be a major cause of cybrid lethality.
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23417419 ???displayArticle.pmcLink???PMC3785129 ???displayArticle.link???Int J Dev Biol ???displayArticle.grants???[+]
Bailey,
Phylogenetic reconstruction of vertebrate Hox cluster duplications.
1997, Pubmed
Bailey,
Phylogenetic reconstruction of vertebrate Hox cluster duplications.
1997,
Pubmed Bascove,
Molecular characterization of Pleurodeles waltl activation-induced cytidine deaminase.
2010,
Pubmed
,
Xenbase Beaujean,
The effect of interspecific oocytes on demethylation of sperm DNA.
2004,
Pubmed Benton,
Paleontological evidence to date the tree of life.
2007,
Pubmed Beyhan,
Interspecies nuclear transfer: implications for embryonic stem cell biology.
2007,
Pubmed Birchler,
A tale of two centromeres--diversity of structure but conservation of function in plants and animals.
2009,
Pubmed Breathnach,
Organization and expression of eucaryotic split genes coding for proteins.
1981,
Pubmed Briggs,
Transplantation of Living Nuclei From Blastula Cells into Enucleated Frogs' Eggs.
1952,
Pubmed Cannon,
Xenomitochondrial mice: investigation into mitochondrial compensatory mechanisms.
2011,
Pubmed Chung,
Reprogramming of human somatic cells using human and animal oocytes.
2009,
Pubmed Dawid,
Deoxyribonucleic acid in amphibian eggs.
1965,
Pubmed Dimitrova,
Regulation of mammalian replication origin usage in Xenopus egg extract.
1998,
Pubmed
,
Xenbase Egli,
Recipient cell nuclear factors are required for reprogramming by nuclear transfer.
2010,
Pubmed ELSDALE,
A description of the technique for nuclear transplantation in Xenopus laevis.
1960,
Pubmed
,
Xenbase Evans,
A mitochondrial DNA phylogeny of African clawed frogs: phylogeography and implications for polyploid evolution.
2004,
Pubmed
,
Xenbase Fujimoto,
Developmental potential of embryonic cells in a nucleocytoplasmic hybrid formed using a goldfish haploid nucleus and loach egg cytoplasm.
2010,
Pubmed Fujiwara,
Uniparental chromosome elimination in the early embryogenesis of the inviable salmonid hybrids between masu salmon female and rainbow trout male.
1997,
Pubmed Gallien,
Nucleocytoplasmic interactions during ontogenesis in individuals obtained by intra- and interspecific nuclear transplantation in the genus Pleurodeles (urodele amphibian).
1973,
Pubmed Ganier,
Synergic reprogramming of mammalian cells by combined exposure to mitotic Xenopus egg extracts and transcription factors.
2011,
Pubmed
,
Xenbase Gómez,
Trichostatin A modified histone covalent pattern and enhanced expression of pluripotent genes in interspecies black-footed cat cloned embryos but did not improve in vitro and in vivo viability.
2011,
Pubmed GURDON,
Tetraploid frogs.
1959,
Pubmed
,
Xenbase GURDON,
The developmental capacity of nuclei taken from differentiating endoderm cells of Xenopus laevis.
1960,
Pubmed
,
Xenbase GURDON,
The transplantation of nuclei between two species of Xenopus.
1962,
Pubmed
,
Xenbase Halley-Stott,
Mammalian nuclear transplantation to Germinal Vesicle stage Xenopus oocytes - a method for quantitative transcriptional reprogramming.
2010,
Pubmed
,
Xenbase HAMILTON,
An experimental analysis of the development of the haploid syndrome in embryos of Xenopus laevis.
1963,
Pubmed
,
Xenbase Henikoff,
The centromere paradox: stable inheritance with rapidly evolving DNA.
2001,
Pubmed Hennen,
Back-transfer of late gastrula nuclei of nucleocytoplasmic hybrids.
1974,
Pubmed Hurley,
A new time-scale for ray-finned fish evolution.
2007,
Pubmed Hyrien,
Chromosomal replication initiates and terminates at random sequences but at regular intervals in the ribosomal DNA of Xenopus early embryos.
1993,
Pubmed
,
Xenbase Janvier,
Palaeontology: modern look for ancient lamprey.
2006,
Pubmed Kenyon,
Expanding the functional human mitochondrial DNA database by the establishment of primate xenomitochondrial cybrids.
1997,
Pubmed Kim,
An interclass nuclear transfer between fowl and mammal: in vitro development of chicken-to-cattle interclass embryos and the detection of chicken genetic complements.
2004,
Pubmed Lee,
Enhanced histone acetylation in somatic cells induced by a histone deacetylase inhibitor improved inter-generic cloned leopard cat blastocysts.
2010,
Pubmed Levy,
Nuclear size is regulated by importin α and Ntf2 in Xenopus.
2010,
Pubmed
,
Xenbase Liu,
Blastocysts produced by nuclear transfer between chicken blastodermal cells and rabbit oocytes.
2004,
Pubmed Loi,
Interspecies somatic cell nuclear transfer: a salvage tool seeking first aid.
2011,
Pubmed Loughlin,
Katanin contributes to interspecies spindle length scaling in Xenopus.
2011,
Pubmed
,
Xenbase Luo,
A Jurassic eutherian mammal and divergence of marsupials and placentals.
2011,
Pubmed McKenzie,
Functional respiratory chain analyses in murid xenomitochondrial cybrids expose coevolutionary constraints of cytochrome b and nuclear subunits of complex III.
2003,
Pubmed Mizutani,
Abnormal chromosome segregation at early cleavage is a major cause of the full-term developmental failure of mouse clones.
2012,
Pubmed MOORE,
Transplantation of nuclei between Rana pipiens and Rana sylvatica.
1958,
Pubmed Narbonne,
Deficient induction response in a Xenopus nucleocytoplasmic hybrid.
2011,
Pubmed
,
Xenbase Narbonne,
Reprogramming and development in nuclear transfer embryos and in interspecific systems.
2012,
Pubmed Narbonne,
On the cellular and developmental lethality of a Xenopus nucleocytoplasmic hybrid.
2012,
Pubmed
,
Xenbase Noggle,
Human oocytes reprogram somatic cells to a pluripotent state.
2011,
Pubmed Nomiyama,
A family tree of vertebrate chemokine receptors for a unified nomenclature.
2011,
Pubmed PICHERAL,
[Capacities of the nuclei of embryonic endodermal cells to organize a viable germ in the urodele, Pleurodeles waltlii Michah].
1962,
Pubmed Rajarajan,
Reprogramming of mouse, rat, pig, and human fibroblasts into iPS cells.
2012,
Pubmed Roelants,
Global patterns of diversification in the history of modern amphibians.
2007,
Pubmed Sakai,
Chromosome elimination in the interspecific hybrid medaka between Oryzias latipes and O. hubbsi.
2007,
Pubmed Sha,
Fates of donor and recipient mitochondrial DNA during generation of interspecies SCNT-derived human ES-like cells.
2009,
Pubmed Shi,
The chronological development of the urodele amphibian Pleurodeles waltl (Michah).
1995,
Pubmed
,
Xenbase Shi,
Trichostatin A (TSA) improves the development of rabbit-rabbit intraspecies cloned embryos, but not rabbit-human interspecies cloned embryos.
2008,
Pubmed Sotomaru,
Preimplantation development of somatic cell cloned embryos in the common marmoset (Callithrix jacchus).
2009,
Pubmed Tecirlioglu,
Interspecies somatic cell nuclear transfer and preliminary data for horse-cow/mouse iSCNT.
2006,
Pubmed Wang,
Bovine ooplasm partially remodels primate somatic nuclei following somatic cell nuclear transfer.
2009,
Pubmed Wang,
Reprogrammed transcriptome in rhesus-bovine interspecies somatic cell nuclear transfer embryos.
2011,
Pubmed Wheatley,
Inhibition of chromosomal separation provides insights into cleavage furrow stimulation in cultured epithelial cells.
1998,
Pubmed Woodland,
RNA synthesis in an amphibian nuclear-transplant hybrid.
1969,
Pubmed Yan,
Developmental incompatibility between cell nucleus and cytoplasm as revealed by nuclear transplantation experiments in teleost of different families and orders.
1990,
Pubmed Yan,
Further investigation on nuclear transplantation in different orders of teleost: the combination of the nucleus of Tilapia (Oreochromis nilotica) and the cytoplasm of Loach (Paramisgurnus dabryanus).
1991,
Pubmed Yang,
High-efficiency somatic reprogramming induced by intact MII oocytes.
2010,
Pubmed
