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A role for the dystrophin-glycoprotein complex as a transmembrane linker between laminin and actin.
Ervasti JM, Campbell KP.
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The dystrophin-glycoprotein complex was tested for interaction with several components of the extracellular matrix as well as actin. The 156-kD dystrophin-associated glycoprotein (156-kD dystroglycan) specifically bound laminin in a calcium-dependent manner and was inhibited by NaCl (IC50 = 250 mM) but was not affected by 1,000-fold (wt/wt) excesses of lactose, IKVAV, or YIGSR peptides. Laminin binding was inhibited by heparin (IC50 = 100 micrograms/ml), suggesting that one of the heparin-binding domains of laminin is involved in binding dystroglycan while negatively charged oligosaccharide moieties on dystroglycan were found to be necessary for its laminin-binding activity. No interaction between any component of the dystrophin-glycoprotein complex and fibronectin, collagen I, collagen IV, entactin, or heparan sulfate proteoglycan was detected by 125I-protein overlay and/or extracellular matrix protein-Sepharose precipitation. In addition, laminin-Sepharose quantitatively precipitated purified dystrophin-glycoprotein complex, demonstrating that the laminin-binding site is accessible when dystroglycan is associated with the complex. Dystroglycan of nonmuscle tissues also bound laminin. However, the other proteins of the striated muscledystrophin-glycoprotein complex appear to be absent, antigenically dissimilar or less tightly associated with dystroglycan in nonmuscle tissues. Finally, we show that the dystrophin-glycoprotein complex cosediments with F-actin but does not bind calcium or calmodulin. Our results support a role for the striated muscledystrophin-glycoprotein complex in linking the actin-based cytoskeleton with the extracellular matrix. Furthermore, our results suggest that dystrophin and dystroglycan may play substantially different functional roles in nonmuscle tissues.
Acsadi,
Human dystrophin expression in mdx mice after intramuscular injection of DNA constructs.
1991, Pubmed
Acsadi,
Human dystrophin expression in mdx mice after intramuscular injection of DNA constructs.
1991,
Pubmed al-Hakim,
Electrophoresis and detection of nanogram quantities of exogenous and endogenous glycosaminoglycans in biological fluids.
1991,
Pubmed Anderson,
The molecular and biochemical basis of Duchenne muscular dystrophy.
1992,
Pubmed Aumailley,
Identification of the Arg-Gly-Asp sequence in laminin A chain as a latent cell-binding site being exposed in fragment P1.
1990,
Pubmed Bähler,
Characterization of synapsin I fragments produced by cysteine-specific cleavage: a study of their interactions with F-actin.
1989,
Pubmed Bard,
Extra actin filaments at the periphery of skeletal muscle myofibrils.
1991,
Pubmed Blake,
Characterization of a 4.8kb transcript from the Duchenne muscular dystrophy locus expressed in Schwannoma cells.
1992,
Pubmed Bonilla,
Duchenne muscular dystrophy: deficiency of dystrophin at the muscle cell surface.
1988,
Pubmed Bresnick,
Identification of a short sequence essential for actin binding by Dictyostelium ABP-120.
1990,
Pubmed Burgess,
The large glycoprotein subunit of the skeletal muscle voltage-sensitive calcium channel. Deglycosylation and development.
1988,
Pubmed Byers,
The subcellular distribution of dystrophin in mouse skeletal, cardiac, and smooth muscle.
1991,
Pubmed Campbell,
Identification and characterization of the high affinity [3H]ryanodine receptor of the junctional sarcoplasmic reticulum Ca2+ release channel.
1987,
Pubmed Campbell,
Staining of the Ca2+-binding proteins, calsequestrin, calmodulin, troponin C, and S-100, with the cationic carbocyanine dye "Stains-all".
1983,
Pubmed Clark,
Centractin is an actin homologue associated with the centrosome.
1992,
Pubmed Craig,
Gamma actin, spectrin, and intermediate filament proteins colocalize with vinculin at costameres, myofibril-to-sarcolemma attachment sites.
1983,
Pubmed Dickson,
Co-localization and molecular association of dystrophin with laminin at the surface of mouse and human myotubes.
1992,
Pubmed Douville,
Isolation and partial characterization of high affinity laminin receptors in neural cells.
1988,
Pubmed Duance,
A role for collagen in the pathogenesis of muscular dystrophy?
1980,
Pubmed Dubreuil,
Structure, calmodulin-binding, and calcium-binding properties of recombinant alpha spectrin polypeptides.
1991,
Pubmed Ehrig,
Merosin, a tissue-specific basement membrane protein, is a laminin-like protein.
1990,
Pubmed Engvall,
Distribution and isolation of four laminin variants; tissue restricted distribution of heterotrimers assembled from five different subunits.
1990,
Pubmed Ervasti,
Deficiency of a glycoprotein component of the dystrophin complex in dystrophic muscle.
1990,
Pubmed Ervasti,
Dystrophin and the membrane skeleton.
1993,
Pubmed Ervasti,
Membrane organization of the dystrophin-glycoprotein complex.
1991,
Pubmed Ervasti,
Purification of dystrophin from skeletal muscle.
1991,
Pubmed Flanagan,
Calmodulin-binding proteins: visualization by 125I-calmodulin overlay on blots quenched with Tween 20 or bovine serum albumin and poly(ethylene oxide).
1984,
Pubmed Franco,
Calcium entry through stretch-inactivated ion channels in mdx myotubes.
1990,
Pubmed Goodman,
Locomotory competence and laminin-specific cell surface binding sites are lost during myoblast differentiation.
1989,
Pubmed Graf,
Identification of an amino acid sequence in laminin mediating cell attachment, chemotaxis, and receptor binding.
1987,
Pubmed Hashimoto,
Autophosphorylation of Ca2+/calmodulin-dependent protein kinase II. Effects on total and Ca2+-independent activities and kinetic parameters.
1987,
Pubmed Hemmings,
Analysis of the actin-binding domain of alpha-actinin by mutagenesis and demonstration that dystrophin contains a functionally homologous domain.
1992,
Pubmed Hoffman,
Dystrophin: the protein product of the Duchenne muscular dystrophy locus.
1987,
Pubmed Ibraghimov-Beskrovnaya,
Primary structure of dystrophin-associated glycoproteins linking dystrophin to the extracellular matrix.
1992,
Pubmed Imagawa,
Purified ryanodine receptor from skeletal muscle sarcoplasmic reticulum is the Ca2+-permeable pore of the calcium release channel.
1987,
Pubmed Jorgensen,
Identification of novel proteins unique to either transverse tubules (TS28) or the sarcolemma (SL50) in rabbit skeletal muscle.
1990,
Pubmed Karinch,
The identification and sequence of the actin-binding domain of human red blood cell beta-spectrin.
1990,
Pubmed King,
The visualization of human erythrocyte membrane proteins and glycoproteins in SDS polyacrylamide gels employing a single staining procedure.
1976,
Pubmed Kinne,
Keratan sulfate proteoglycan in rabbit compact bone is bone sialoprotein II.
1987,
Pubmed Klietsch,
Dystrophin-glycoprotein complex and laminin colocalize to the sarcolemma and transverse tubules of cardiac muscle.
1993,
Pubmed Koenig,
The complete sequence of dystrophin predicts a rod-shaped cytoskeletal protein.
1988,
Pubmed Kouzi-Koliakos,
Mapping of three major heparin-binding sites on laminin and identification of a novel heparin-binding site on the B1 chain.
1989,
Pubmed Lakonishok,
The alpha 5 beta 1 integrin associates with a dystrophin-containing lattice during muscle development.
1992,
Pubmed Lederfein,
A 71-kilodalton protein is a major product of the Duchenne muscular dystrophy gene in brain and other nonmuscle tissues.
1992,
Pubmed Lee,
Expression of recombinant dystrophin and its localization to the cell membrane.
1991,
Pubmed Lees-Miller,
A vertebrate actin-related protein is a component of a multisubunit complex involved in microtubule-based vesicle motility.
1992,
Pubmed Lesot,
Isolation of a laminin-binding protein from muscle cell membranes.
1983,
Pubmed Lev,
Expression of the Duchenne's muscular dystrophy gene in cultured muscle cells.
1987,
Pubmed Levine,
Binding sites involved in the interaction of actin with the N-terminal region of dystrophin.
1992,
Pubmed Lidov,
Localization of dystrophin to postsynaptic regions of central nervous system cortical neurons.
,
Pubmed Linhardt,
Isolation and characterization of human heparin.
1992,
Pubmed Love,
An autosomal transcript in skeletal muscle with homology to dystrophin.
1989,
Pubmed Madhavan,
Calmodulin specifically binds three proteins of the dystrophin-glycoprotein complex.
1992,
Pubmed Marshall,
Accumulation of collagen and altered fiber-type ratios as indicators of abnormal muscle gene expression in the mdx dystrophic mouse.
1989,
Pubmed Matsumura,
Abnormal expression of dystrophin-associated proteins in Fukuyama-type congenital muscular dystrophy.
1993,
Pubmed Matsumura,
Association of dystrophin-related protein with dystrophin-associated proteins in mdx mouse muscle.
1992,
Pubmed Matsumura,
Deficiency of the 50K dystrophin-associated glycoprotein in severe childhood autosomal recessive muscular dystrophy.
1992,
Pubmed Mecham,
Receptors for laminin on mammalian cells.
1991,
Pubmed Menke,
Decreased osmotic stability of dystrophin-less muscle cells from the mdx mouse.
1991,
Pubmed Ohlendieck,
Dystrophin-related protein is localized to neuromuscular junctions of adult skeletal muscle.
1991,
Pubmed Ohlendieck,
Dystrophin-associated proteins are greatly reduced in skeletal muscle from mdx mice.
1991,
Pubmed Ohlendieck,
Duchenne muscular dystrophy: deficiency of dystrophin-associated proteins in the sarcolemma.
1993,
Pubmed Ohlendieck,
Dystrophin constitutes 5% of membrane cytoskeleton in skeletal muscle.
1991,
Pubmed Ohlendieck,
Dystrophin-glycoprotein complex is highly enriched in isolated skeletal muscle sarcolemma.
1991,
Pubmed Otey,
An interaction between alpha-actinin and the beta 1 integrin subunit in vitro.
1990,
Pubmed Paulsson,
Structure of laminin variants. The 300-kDa chains of murine and bovine heart laminin are related to the human placenta merosin heavy chain and replace the a chain in some laminin variants.
1991,
Pubmed Porter,
Dystrophin colocalizes with beta-spectrin in distinct subsarcolemmal domains in mammalian skeletal muscle.
1992,
Pubmed Rampoldi,
A comparative analysis of collagen III, IV, laminin and fibronectin in Duchenne muscular dystrophy biopsies and cell cultures.
1986,
Pubmed Sanes,
Molecular heterogeneity of basal laminae: isoforms of laminin and collagen IV at the neuromuscular junction and elsewhere.
1990,
Pubmed Sealock,
Localization of dystrophin relative to acetylcholine receptor domains in electric tissue and adult and cultured skeletal muscle.
1991,
Pubmed
,
Xenbase Sharp,
Identification and characterization of the dihydropyridine-binding subunit of the skeletal muscle dihydropyridine receptor.
1987,
Pubmed Skubitz,
Localization of three distinct heparin-binding domains of laminin by monoclonal antibodies.
1988,
Pubmed Smalheiser,
Cranin: a laminin-binding protein of cell membranes.
1987,
Pubmed Sonnenberg,
Isolation of alpha 6 beta 1 integrins from platelets and adherent cells by affinity chromatography on mouse laminin fragment E8 and human laminin pepsin fragment.
1991,
Pubmed Soroka,
Characterization of a novel heparan sulfate proteoglycan found in the extracellular matrix of liver sinusoids and basement membranes.
1991,
Pubmed Stedman,
The mdx mouse diaphragm reproduces the degenerative changes of Duchenne muscular dystrophy.
1991,
Pubmed Tashiro,
A synthetic peptide containing the IKVAV sequence from the A chain of laminin mediates cell attachment, migration, and neurite outgrowth.
1989,
Pubmed Timpl,
Basement membranes.
1987,
Pubmed Towbin,
Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.
1979,
Pubmed Turner,
Increased calcium influx in dystrophic muscle.
1991,
Pubmed von der Mark,
Skeletal myoblasts utilize a novel beta 1-series integrin and not alpha 6 beta 1 for binding to the E8 and T8 fragments of laminin.
1991,
Pubmed Wallis,
Characterization of calcium binding to brain spectrin.
1992,
Pubmed Watkins,
Immunoelectron microscopic localization of dystrophin in myofibres.
1988,
Pubmed Way,
Expression of the N-terminal domain of dystrophin in E. coli and demonstration of binding to F-actin.
1992,
Pubmed Weller,
Dystrophin-deficient mdx muscle fibers are preferentially vulnerable to necrosis induced by experimental lengthening contractions.
1990,
Pubmed Wu,
Mucin-like glycoprotein secreted by cultured hamster tracheal epithelial cells. Biochemical and immunological characterization.
1991,
Pubmed Yamada,
Adhesive recognition sequences.
1991,
Pubmed Yeadon,
Dystrophin is a component of the subsynaptic membrane.
1991,
Pubmed Yoshida,
Glycoprotein complex anchoring dystrophin to sarcolemma.
1990,
Pubmed
