Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Proc Natl Acad Sci U S A
2013 Mar 26;11013:5217-22. doi: 10.1073/pnas.1219611110.
Show Gene links
Show Anatomy links
State-dependent FRET reports calcium- and voltage-dependent gating-ring motions in BK channels.
Miranda P, Contreras JE, Plested AJ, Sigworth FJ, Holmgren M, Giraldez T.
???displayArticle.abstract???
Large-conductance voltage- and calcium-dependent potassium channels (BK, "Big K+") are important controllers of cell excitability. In the BK channel, a large C-terminal intracellular region containing a "gating-ring" structure has been proposed to transduce Ca(2+) binding into channel opening. Using patch-clamp fluorometry, we have investigated the calcium and voltage dependence of conformational changes of the gating-ring region of BK channels, while simultaneously monitoring channel conductance. Fluorescence resonance energy transfer (FRET) between fluorescent protein inserts indicates that Ca(2+) binding produces structural changes of the gating ring that are much larger than those predicted by current X-ray crystal structures of isolated gating rings.
Bao,
Mapping the BKCa channel's "Ca2+ bowl": side-chains essential for Ca2+ sensing.
2004, Pubmed,
Xenbase
Bao,
Mapping the BKCa channel's "Ca2+ bowl": side-chains essential for Ca2+ sensing.
2004,
Pubmed
,
Xenbase Bao,
Elimination of the BK(Ca) channel's high-affinity Ca(2+) sensitivity.
2002,
Pubmed
,
Xenbase Bers,
A practical guide to the preparation of Ca2+ buffers.
1994,
Pubmed Bian,
Ca2+-binding activity of a COOH-terminal fragment of the Drosophila BK channel involved in Ca2+-dependent activation.
2001,
Pubmed Bloch,
KCNMA1 gene amplification promotes tumor cell proliferation in human prostate cancer.
2007,
Pubmed Cha,
Structural implications of fluorescence quenching in the Shaker K+ channel.
1998,
Pubmed Cox,
Allosteric gating of a large conductance Ca-activated K+ channel.
1997,
Pubmed
,
Xenbase Cui,
Allosteric linkage between voltage and Ca(2+)-dependent activation of BK-type mslo1 K(+) channels.
2000,
Pubmed Cui,
Molecular mechanisms of BK channel activation.
2009,
Pubmed Dale,
The orientational freedom of molecular probes. The orientation factor in intramolecular energy transfer.
1979,
Pubmed Díaz,
Role of the S4 segment in a voltage-dependent calcium-sensitive potassium (hSlo) channel.
1998,
Pubmed
,
Xenbase Giraldez,
Generation of functional fluorescent BK channels by random insertion of GFP variants.
2005,
Pubmed Grimm,
Hypertension of Kcnmb1-/- is linked to deficient K secretion and aldosteronism.
2009,
Pubmed Heim,
Green fluorescent protein forms for energy transfer.
1999,
Pubmed Hink,
Structural dynamics of green fluorescent protein alone and fused with a single chain Fv protein.
2000,
Pubmed Horrigan,
Coupling between voltage sensor activation, Ca2+ binding and channel opening in large conductance (BK) potassium channels.
2002,
Pubmed
,
Xenbase Hu,
Effects of multiple metal binding sites on calcium and magnesium-dependent activation of BK channels.
2006,
Pubmed
,
Xenbase Javaherian,
Metal-driven operation of the human large-conductance voltage- and Ca2+-dependent potassium channel (BK) gating ring apparatus.
2011,
Pubmed Jiang,
Crystal structure and mechanism of a calcium-gated potassium channel.
2002,
Pubmed Latorre,
Large conductance Ca2+-activated K+ (BK) channel: activation by Ca2+ and voltage.
2006,
Pubmed Lingle,
Gating rings formed by RCK domains: keys to gate opening.
2007,
Pubmed Qian,
Intra- and intersubunit cooperativity in activation of BK channels by Ca2+.
2006,
Pubmed
,
Xenbase Rothberg,
Voltage and Ca2+ activation of single large-conductance Ca2+-activated K+ channels described by a two-tiered allosteric gating mechanism.
2000,
Pubmed Schreiber,
Transplantable sites confer calcium sensitivity to BK channels.
1999,
Pubmed
,
Xenbase Schreiber,
A novel calcium-sensing domain in the BK channel.
1997,
Pubmed
,
Xenbase Stefani,
Voltage-controlled gating in a large conductance Ca2+-sensitive K+channel (hslo).
1997,
Pubmed
,
Xenbase Sweet,
Measurements of the BKCa channel's high-affinity Ca2+ binding constants: effects of membrane voltage.
2008,
Pubmed Taraska,
Structural dynamics in the gating ring of cyclic nucleotide-gated ion channels.
2007,
Pubmed Toro,
Maxi-K(Ca), a Unique Member of the Voltage-Gated K Channel Superfamily.
1998,
Pubmed Villalba-Galea,
S4-based voltage sensors have three major conformations.
2008,
Pubmed Wang,
Structure of the BK potassium channel in a lipid membrane from electron cryomicroscopy.
2009,
Pubmed Wei,
Calcium sensitivity of BK-type KCa channels determined by a separable domain.
1994,
Pubmed Winn,
Overview of the CCP4 suite and current developments.
2011,
Pubmed Wu,
Structure of the gating ring from the human large-conductance Ca(2+)-gated K(+) channel.
2010,
Pubmed Xia,
Multiple regulatory sites in large-conductance calcium-activated potassium channels.
2002,
Pubmed Yang,
An epilepsy/dyskinesia-associated mutation enhances BK channel activation by potentiating Ca2+ sensing.
2010,
Pubmed
,
Xenbase Yuan,
Structure of the human BK channel Ca2+-activation apparatus at 3.0 A resolution.
2010,
Pubmed Yuan,
Open structure of the Ca2+ gating ring in the high-conductance Ca2+-activated K+ channel.
2011,
Pubmed Zhang,
Ion sensing in the RCK1 domain of BK channels.
2010,
Pubmed
,
Xenbase Zheng,
Patch-clamp fluorometry recording of conformational rearrangements of ion channels.
2003,
Pubmed
,
Xenbase Zheng,
Disruption of an intersubunit interaction underlies Ca2+-calmodulin modulation of cyclic nucleotide-gated channels.
2003,
Pubmed
,
Xenbase
