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The developmental relationship between the blood and endothelial cell (EC) lineages remains unclear. In the extra-embryonic blood islands of birds and mammals, ECs and blood cells are closely intermixed, and blood island precursor cells in the primitive streak express many of the same molecular markers, leading to the suggestion that both lineages arise from a common precursor, called the hemangioblast. Cells within the blood island of Xenopus also coexpress predifferentiation markers of the blood and EC lineages. However, using multiple assays, we find that precursor cells in the Xenopus blood island do not normally differentiate into ECs, suggesting that classic hemangioblasts are rare or nonexistent in Xenopus. What prevents these precursor cells from developing into mature ECs? We have found that bone morphogenetic protein (BMP) signaling is essential for erythroid differentiation, and in the absence of BMP signaling, precursor cells adopt an EC fate. Furthermore, inhibition of the erythroid transcription pathway leads to endothelial differentiation. Our results indicate that bipotential endothelial/erythroid precursor cells do indeed exist in the Xenopus blood island, but BMP signaling normally acts to constrain EC fate. More generally, these results provide evidence that commitment to the erythroid lineage limits development of bipotential precursors toward an endothelial fate.
Amaya,
The hemangioblast: a state of competence.
2013, Pubmed,
Xenbase
Amaya,
The hemangioblast: a state of competence.
2013,
Pubmed
,
Xenbase Choi,
A common precursor for hematopoietic and endothelial cells.
1998,
Pubmed Ciau-Uitz,
Genetic control of hematopoietic development in Xenopus and zebrafish.
2010,
Pubmed
,
Xenbase Cleaver,
Neovascularization of the Xenopus embryo.
1997,
Pubmed
,
Xenbase Coffin,
Embryonic vascular development: immunohistochemical identification of the origin and subsequent morphogenesis of the major vessel primordia in quail embryos.
1988,
Pubmed Costa,
spib is required for primitive myeloid development in Xenopus.
2008,
Pubmed
,
Xenbase Dalgin,
GATA-2 functions downstream of BMPs and CaM KIV in ectodermal cells during primitive hematopoiesis.
2007,
Pubmed
,
Xenbase Deimling,
Fgf is required to regulate anterior-posterior patterning in the Xenopus lateral plate mesoderm.
2011,
Pubmed
,
Xenbase Drogat,
Vegf regulates embryonic erythroid development through Gata1 modulation.
2010,
Pubmed Huber,
Haemangioblast commitment is initiated in the primitive streak of the mouse embryo.
2004,
Pubmed Kallianpur,
The SCL/TAL-1 gene is expressed in progenitors of both the hematopoietic and vascular systems during embryogenesis.
1994,
Pubmed Kazanskaya,
The Wnt signaling regulator R-spondin 3 promotes angioblast and vascular development.
2008,
Pubmed
,
Xenbase Kennedy,
A common precursor for primitive erythropoiesis and definitive haematopoiesis.
1997,
Pubmed Kennedy,
Development of the hemangioblast defines the onset of hematopoiesis in human ES cell differentiation cultures.
2007,
Pubmed Kinder,
The orderly allocation of mesodermal cells to the extraembryonic structures and the anteroposterior axis during gastrulation of the mouse embryo.
1999,
Pubmed Koibuchi,
The effect of VEGF on blood vessels and blood cells during Xenopus development.
2006,
Pubmed
,
Xenbase Lee,
Notch signaling functions as a cell-fate switch between the endothelial and hematopoietic lineages.
2009,
Pubmed Lee,
ER71 acts downstream of BMP, Notch, and Wnt signaling in blood and vessel progenitor specification.
2008,
Pubmed Leung,
Vascular endothelial growth factor is a secreted angiogenic mitogen.
1989,
Pubmed Liang,
The role of vascular endothelial growth factor (VEGF) in vasculogenesis, angiogenesis, and hematopoiesis in zebrafish development.
2001,
Pubmed Liu,
Fli1 acts at the top of the transcriptional network driving blood and endothelial development.
2008,
Pubmed
,
Xenbase Meadows,
Kruppel-like factor 2 cooperates with the ETS family protein ERG to activate Flk1 expression during vascular development.
2009,
Pubmed
,
Xenbase Meijering,
Methods for cell and particle tracking.
2012,
Pubmed Nakazawa,
Negative regulation of primitive hematopoiesis by the FGF signaling pathway.
2006,
Pubmed Nishikawa,
Hemangioblast: an in vitro phantom.
2012,
Pubmed Nishikawa,
Progressive lineage analysis by cell sorting and culture identifies FLK1+VE-cadherin+ cells at a diverging point of endothelial and hemopoietic lineages.
1998,
Pubmed Rhee,
Mapping mouse hemangioblast maturation from headfold stages.
2012,
Pubmed Rupp,
Novel approaches for the study of vascular assembly and morphogenesis in avian embryos.
2003,
Pubmed Sabin,
Preliminary note on the differentiation of angioblasts and the method by which they produce blood-vessels, blood-plasma and red blood-cells as seen in the living chick. 1917.
2002,
Pubmed Sadlon,
BMP4: its role in development of the hematopoietic system and potential as a hematopoietic growth factor.
2004,
Pubmed
,
Xenbase Salanga,
ETS family protein ETV2 is required for initiation of the endothelial lineage but not the hematopoietic lineage in the Xenopus embryo.
2010,
Pubmed
,
Xenbase Shivdasani,
Absence of blood formation in mice lacking the T-cell leukaemia oncoprotein tal-1/SCL.
1995,
Pubmed Simões,
Fgf differentially controls cross-antagonism between cardiac and haemangioblast regulators.
2011,
Pubmed Smith,
Expression cloning of noggin, a new dorsalizing factor localized to the Spemann organizer in Xenopus embryos.
1992,
Pubmed
,
Xenbase Söderberg,
Complex and context dependent regulation of hematopoiesis by TGF-beta superfamily signaling.
2009,
Pubmed Sumanas,
Interplay among Etsrp/ER71, Scl, and Alk8 signaling controls endothelial and myeloid cell formation.
2008,
Pubmed Tran,
Wnt/beta-catenin signaling is involved in the induction and maintenance of primitive hematopoiesis in the vertebrate embryo.
2010,
Pubmed
,
Xenbase Tripic,
SCL and associated proteins distinguish active from repressive GATA transcription factor complexes.
2009,
Pubmed Tsai,
An early haematopoietic defect in mice lacking the transcription factor GATA-2.
1994,
Pubmed Ueno,
Clonal analysis of mouse development reveals a polyclonal origin for yolk sac blood islands.
2006,
Pubmed Vogeli,
A common progenitor for haematopoietic and endothelial lineages in the zebrafish gastrula.
2006,
Pubmed Wallingford,
Preparation of fixed Xenopus embryos for confocal imaging.
2010,
Pubmed
,
Xenbase Walmsley,
Adult and embryonic blood and endothelium derive from distinct precursor populations which are differentially programmed by BMP in Xenopus.
2002,
Pubmed
,
Xenbase Walters,
Calmodulin-dependent protein kinase IV mediated antagonism of BMP signaling regulates lineage and survival of hematopoietic progenitors.
2002,
Pubmed
,
Xenbase Warren,
The oncogenic cysteine-rich LIM domain protein rbtn2 is essential for erythroid development.
1994,
Pubmed Weng,
On hemangioblasts in chicken.
2007,
Pubmed Winnier,
Bone morphogenetic protein-4 is required for mesoderm formation and patterning in the mouse.
1995,
Pubmed
