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Nuclear shape and size vary between species, during development, and in many tissue pathologies, but the causes and effects of these differences remain poorly understood. During fertilization, sperm nuclei undergo a dramatic conversion from a heavily compacted form into decondensed, spherical pronuclei, accompanied by rapid nucleation of microtubules from centrosomes. Here we report that the assembly of the spherical nucleus depends on a critical balance of microtubule dynamics, which is regulated by the chromatin-binding protein Developmental pluripotency-associated 2 (Dppa2). Whereas microtubules normally promote sperm pronuclear expansion, in Dppa2-depleted Xenopus egg extracts excess microtubules cause pronuclear assembly defects, leading to abnormal morphology and disorganized DNA replication. Dppa2 inhibits microtubule polymerization in vitro, and Dppa2 activity is needed at a precise time and location during nascent pronuclear formation. This demonstrates a strict spatiotemporal requirement for local suppression of microtubules during nuclear formation, fulfilled by chromatin-bound microtubule regulators.
Athale,
Regulation of microtubule dynamics by reaction cascades around chromosomes.
2008, Pubmed,
Xenbase
Athale,
Regulation of microtubule dynamics by reaction cascades around chromosomes.
2008,
Pubmed
,
Xenbase Beaudouin,
Nuclear envelope breakdown proceeds by microtubule-induced tearing of the lamina.
2002,
Pubmed Belmont,
Real-time visualization of cell cycle-dependent changes in microtubule dynamics in cytoplasmic extracts.
1990,
Pubmed
,
Xenbase Blow,
Replication origins in Xenopus egg extract Are 5-15 kilobases apart and are activated in clusters that fire at different times.
2001,
Pubmed
,
Xenbase Brandt,
Developmental control of nuclear size and shape by Kugelkern and Kurzkern.
2006,
Pubmed
,
Xenbase Budde,
Analysis of microtubule polymerization in vitro and during the cell cycle in Xenopus egg extracts.
2006,
Pubmed
,
Xenbase Budde,
Regulation of Op18 during spindle assembly in Xenopus egg extracts.
2001,
Pubmed
,
Xenbase Caudron,
Spatial coordination of spindle assembly by chromosome-mediated signaling gradients.
2005,
Pubmed
,
Xenbase Dobrynin,
Cdc48/p97-Ufd1-Npl4 antagonizes Aurora B during chromosome segregation in HeLa cells.
2011,
Pubmed
,
Xenbase Ewald,
Microtubule-dependent assembly of the nuclear envelope in Xenopus laevis egg extract.
2001,
Pubmed
,
Xenbase Forbes,
Spontaneous formation of nucleus-like structures around bacteriophage DNA microinjected into Xenopus eggs.
1983,
Pubmed
,
Xenbase Funabiki,
The Xenopus chromokinesin Xkid is essential for metaphase chromosome alignment and must be degraded to allow anaphase chromosome movement.
2000,
Pubmed
,
Xenbase Gadea,
Aurora B is required for mitotic chromatin-induced phosphorylation of Op18/Stathmin.
2006,
Pubmed
,
Xenbase Gruss,
Ran induces spindle assembly by reversing the inhibitory effect of importin alpha on TPX2 activity.
2001,
Pubmed
,
Xenbase Guo,
Sculpting the nucleus with dynamic microtubules.
2013,
Pubmed
,
Xenbase Haithcock,
Age-related changes of nuclear architecture in Caenorhabditis elegans.
2005,
Pubmed Haraguchi,
Live cell imaging and electron microscopy reveal dynamic processes of BAF-directed nuclear envelope assembly.
2008,
Pubmed Harel,
Importin beta negatively regulates nuclear membrane fusion and nuclear pore complex assembly.
2003,
Pubmed
,
Xenbase Heald,
Self-organization of microtubules into bipolar spindles around artificial chromosomes in Xenopus egg extracts.
1996,
Pubmed
,
Xenbase Hetzer,
GTP hydrolysis by Ran is required for nuclear envelope assembly.
2000,
Pubmed
,
Xenbase Houghtaling,
Op18 reveals the contribution of nonkinetochore microtubules to the dynamic organization of the vertebrate meiotic spindle.
2009,
Pubmed
,
Xenbase Kalab,
Visualization of a Ran-GTP gradient in interphase and mitotic Xenopus egg extracts.
2002,
Pubmed
,
Xenbase Kelly,
Survivin reads phosphorylated histone H3 threonine 3 to activate the mitotic kinase Aurora B.
2010,
Pubmed
,
Xenbase Kelly,
Chromosomal enrichment and activation of the aurora B pathway are coupled to spatially regulate spindle assembly.
2007,
Pubmed
,
Xenbase Kinoshita,
Reconstitution of physiological microtubule dynamics using purified components.
2001,
Pubmed
,
Xenbase Lan,
Aurora B phosphorylates centromeric MCAK and regulates its localization and microtubule depolymerization activity.
2004,
Pubmed
,
Xenbase Lemaitre,
Dynamics of the genome during early Xenopus laevis development: karyomeres as independent units of replication.
1998,
Pubmed
,
Xenbase Levy,
Nuclear size is regulated by importin α and Ntf2 in Xenopus.
2010,
Pubmed
,
Xenbase Lu,
Formation of the postmitotic nuclear envelope from extended ER cisternae precedes nuclear pore assembly.
2011,
Pubmed Maldonado-Saldivia,
Dppa2 and Dppa4 are closely linked SAP motif genes restricted to pluripotent cells and the germ line.
2007,
Pubmed Maresca,
Spindle assembly in the absence of a RanGTP gradient requires localized CPC activity.
2009,
Pubmed
,
Xenbase McGarry,
Geminin, an inhibitor of DNA replication, is degraded during mitosis.
1998,
Pubmed
,
Xenbase Meyerzon,
Centrosome attachment to the C. elegans male pronucleus is dependent on the surface area of the nuclear envelope.
2009,
Pubmed Minshull,
A MAP kinase-dependent spindle assembly checkpoint in Xenopus egg extracts.
1994,
Pubmed
,
Xenbase Murray,
Cell cycle extracts.
1991,
Pubmed Nachury,
Importin beta is a mitotic target of the small GTPase Ran in spindle assembly.
2001,
Pubmed
,
Xenbase Newport,
Nuclear reconstitution in vitro: stages of assembly around protein-free DNA.
1987,
Pubmed
,
Xenbase Niethammer,
Discrete states of a protein interaction network govern interphase and mitotic microtubule dynamics.
2007,
Pubmed
,
Xenbase Philpott,
Sperm decondensation in Xenopus egg cytoplasm is mediated by nucleoplasmin.
1991,
Pubmed
,
Xenbase Postow,
Ku80 removal from DNA through double strand break-induced ubiquitylation.
2008,
Pubmed
,
Xenbase Ramadan,
Cdc48/p97 promotes reformation of the nucleus by extracting the kinase Aurora B from chromatin.
2007,
Pubmed
,
Xenbase Salina,
Cytoplasmic dynein as a facilitator of nuclear envelope breakdown.
2002,
Pubmed Sampath,
The chromosomal passenger complex is required for chromatin-induced microtubule stabilization and spindle assembly.
2004,
Pubmed
,
Xenbase Schoft,
The lamina-associated polypeptide 2 (LAP2) isoforms beta, gamma and omega of zebrafish: developmental expression and behavior during the cell cycle.
2003,
Pubmed
,
Xenbase Schuldt,
Nuclear organization: Microtubule shaping of the nucleus.
2013,
Pubmed
,
Xenbase Siegel,
Functional dissection of XDppa2/4 structural domains in Xenopus development.
2009,
Pubmed
,
Xenbase Solovei,
LBR and lamin A/C sequentially tether peripheral heterochromatin and inversely regulate differentiation.
2013,
Pubmed Solovei,
Nuclear architecture of rod photoreceptor cells adapts to vision in mammalian evolution.
2009,
Pubmed Tseng,
Dual detection of chromosomes and microtubules by the chromosomal passenger complex drives spindle assembly.
2010,
Pubmed
,
Xenbase Verde,
Control of microtubule dynamics and length by cyclin A- and cyclin B-dependent kinases in Xenopus egg extracts.
1992,
Pubmed
,
Xenbase Verde,
Regulation of microtubule dynamics by cdc2 protein kinase in cell-free extracts of Xenopus eggs.
1990,
Pubmed
,
Xenbase Vigneron,
Kinetochore localization of spindle checkpoint proteins: who controls whom?
2004,
Pubmed
,
Xenbase Walther,
RanGTP mediates nuclear pore complex assembly.
2003,
Pubmed
,
Xenbase Wang,
Mechanotransduction at a distance: mechanically coupling the extracellular matrix with the nucleus.
2009,
Pubmed Wang,
Histone H3 Thr-3 phosphorylation by Haspin positions Aurora B at centromeres in mitosis.
2010,
Pubmed
,
Xenbase Waterman-Storer,
Membrane/microtubule tip attachment complexes (TACs) allow the assembly dynamics of plus ends to push and pull membranes into tubulovesicular networks in interphase Xenopus egg extracts.
1995,
Pubmed
,
Xenbase Webster,
Sizing up the nucleus: nuclear shape, size and nuclear-envelope assembly.
2009,
Pubmed Wiese,
Role of importin-beta in coupling Ran to downstream targets in microtubule assembly.
2001,
Pubmed
,
Xenbase Wright,
Sperm nuclear activation during fertilization.
1999,
Pubmed Wühr,
How does a millimeter-sized cell find its center?
2009,
Pubmed
,
Xenbase Yamagishi,
Two histone marks establish the inner centromere and chromosome bi-orientation.
2010,
Pubmed Zhang,
Chromatin-independent nuclear envelope assembly induced by Ran GTPase in Xenopus egg extracts.
2000,
Pubmed
,
Xenbase Zink,
Nuclear structure in cancer cells.
2004,
Pubmed
