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XB-ART-47931
Cold Spring Harb Protoc 2013 Sep 01;20139:869-72. doi: 10.1101/pdb.prot077149.
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Labeling individual neurons in the brains of live Xenopus tadpoles by electroporation of dyes or DNA.

Ruthazer ES, Schohl A, Schwartz N, Tavakoli A, Tremblay M, Cline HT.


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This protocol describes the targeted introduction of fluorophore in the form of a dye or genetic material into single cells. This method has the advantage of producing true single-cell chimeric animals in which to study the effects of overexpression or knockdown of a gene in an otherwise entirely wild-type background.

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