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Eggs and oocytes have a remarkable ability to induce transcription of sperm after normal fertilization and in somatic nuclei after somatic cell nuclear transfer. This ability of eggs and oocytes is essential for normal development. Nuclear actin and actin-binding proteins have been shown to contribute to transcription, although their mode of action is elusive. Here, we find that Xenopus Wave1, previously characterized as a protein involved in actin cytoskeleton organization, is present in the oocytenucleus and is required for efficient transcriptional reprogramming. Moreover, Wave1 knockdown in embryos results in abnormal development and defective hox gene activation. Nuclear Wave1 binds by its WHD domain to active transcription components, and this binding contributes to the action of RNA polymerase II. We identify Wave1 as a maternal reprogramming factor that also has a necessary role in gene activation in development.
Campellone,
A nucleator arms race: cellular control of actin assembly.
2010, Pubmed
Campellone,
A nucleator arms race: cellular control of actin assembly.
2010,
Pubmed Ferrai,
Induction of HoxB transcription by retinoic acid requires actin polymerization.
2009,
Pubmed Fricke,
Drosophila Cip4/Toca-1 integrates membrane trafficking and actin dynamics through WASP and SCAR/WAVE.
2009,
Pubmed Gao,
Germinal vesicle material is essential for nucleus remodeling after nuclear transfer.
2002,
Pubmed Gurdon,
Changes in somatic cell nuclei inserted into growing and maturing amphibian oocytes.
1968,
Pubmed
,
Xenbase Ho,
Toca-1 mediates Cdc42-dependent actin nucleation by activating the N-WASP-WIP complex.
2004,
Pubmed
,
Xenbase Hulstrand,
The use of antisense oligonucleotides in Xenopus oocytes.
2010,
Pubmed
,
Xenbase Jullien,
Mechanisms of nuclear reprogramming by eggs and oocytes: a deterministic process?
2011,
Pubmed
,
Xenbase Kim,
Epigenetic memory in induced pluripotent stem cells.
2010,
Pubmed Koche,
Reprogramming factor expression initiates widespread targeted chromatin remodeling.
2011,
Pubmed Miki,
WAVE, a novel WASP-family protein involved in actin reorganization induced by Rac.
1998,
Pubmed Miyamoto,
Nuclear actin polymerization is required for transcriptional reprogramming of Oct4 by oocytes.
2011,
Pubmed
,
Xenbase Ogushi,
The maternal nucleolus is essential for early embryonic development in mammals.
2008,
Pubmed Rawe,
WAVE1 intranuclear trafficking is essential for genomic and cytoskeletal dynamics during fertilization: cell-cycle-dependent shuttling between M-phase and interphase nuclei.
2004,
Pubmed Takenawa,
The WASP-WAVE protein network: connecting the membrane to the cytoskeleton.
2007,
Pubmed Taylor,
Nuclear role of WASp in the pathogenesis of dysregulated TH1 immunity in human Wiskott-Aldrich syndrome.
2010,
Pubmed Wu,
Regulation of RNA-polymerase-II-dependent transcription by N-WASP and its nuclear-binding partners.
2006,
Pubmed Yokoyama,
Leukemia proto-oncoprotein MLL is proteolytically processed into 2 fragments with opposite transcriptional properties.
2002,
Pubmed
