Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Nat Cell Biol
2013 Dec 01;1512:1434-44. doi: 10.1038/ncb2880.
Show Gene links
Show Anatomy links
The Cep63 paralogue Deup1 enables massive de novo centriole biogenesis for vertebrate multiciliogenesis.
Zhao H, Zhu L, Zhu Y, Cao J, Li S, Huang Q, Xu T, Huang X, Yan X, Zhu X.
???displayArticle.abstract???
Dense multicilia in higher vertebrates are important for luminal flow and the removal of thick mucus. To generate hundreds of basal bodies for multiciliogenesis, specialized terminally differentiated epithelial cells undergo massive centriole amplification. In proliferating cells, however, centriole duplication occurs only once per cell cycle. How cells ensure proper regulation of centriole biogenesis in different contexts is poorly understood. We report that the centriole amplification is controlled by two duplicated genes, Cep63 and Deup1. Cep63 regulates mother-centriole-dependent centriole duplication. Deup1 governs deuterosome assembly to mediate large-scale de novo centriole biogenesis. Similarly to Cep63, Deup1 binds to Cep152 and then recruits Plk4 to activate centriole biogenesis. Phylogenetic analyses suggest that Deup1 diverged from Cep63 in a certain ancestor of lobe-finned fishes during vertebrate evolution and was subsequently adopted by tetrapods. Thus, the Cep63 gene duplication has enabled mother-centriole-independent assembly of the centriole duplication machinery to satisfy different requirements for centriole number.
Amemiya,
The African coelacanth genome provides insights into tetrapod evolution.
2013, Pubmed
Amemiya,
The African coelacanth genome provides insights into tetrapod evolution.
2013,
Pubmed Anderson,
The formation of basal bodies (centrioles) in the Rhesus monkey oviduct.
1971,
Pubmed Arquint,
Cell-cycle-regulated expression of STIL controls centriole number in human cells.
2012,
Pubmed Avidor-Reiss,
Building a centriole.
2013,
Pubmed Azimzadeh,
Building the centriole.
2010,
Pubmed Azimzadeh,
Centrosome loss in the evolution of planarians.
2012,
Pubmed Beisson,
Basal body/centriole assembly and continuity.
2003,
Pubmed Bertrand,
Evolutionary crossroads in developmental biology: amphioxus.
2011,
Pubmed Blacque,
Loss of C. elegans BBS-7 and BBS-8 protein function results in cilia defects and compromised intraflagellar transport.
2004,
Pubmed Boulter,
Regulation of cell-matrix adhesion dynamics and Rac-1 by integrin linked kinase.
2006,
Pubmed Cañestro,
Seeing chordate evolution through the Ciona genome sequence.
2003,
Pubmed Cao,
miR-129-3p controls cilia assembly by regulating CP110 and actin dynamics.
2012,
Pubmed Cizmecioglu,
Cep152 acts as a scaffold for recruitment of Plk4 and CPAP to the centrosome.
2010,
Pubmed Cunha-Ferreira,
From zero to many: control of centriole number in development and disease.
2009,
Pubmed Dirksen,
Centriole morphogenesis in developing ciliated epithelium of the mouse oviduct.
1971,
Pubmed Dzhindzhev,
Asterless is a scaffold for the onset of centriole assembly.
2010,
Pubmed Fliegauf,
When cilia go bad: cilia defects and ciliopathies.
2007,
Pubmed Gönczy,
Towards a molecular architecture of centriole assembly.
2012,
Pubmed Habedanck,
The Polo kinase Plk4 functions in centriole duplication.
2005,
Pubmed Hatch,
Cep152 interacts with Plk4 and is required for centriole duplication.
2010,
Pubmed
,
Xenbase Hayes,
Identification of novel ciliogenesis factors using a new in vivo model for mucociliary epithelial development.
2007,
Pubmed
,
Xenbase Kleylein-Sohn,
Plk4-induced centriole biogenesis in human cells.
2007,
Pubmed Kohlmaier,
Overly long centrioles and defective cell division upon excess of the SAS-4-related protein CPAP.
2009,
Pubmed Kramer-Zucker,
Cilia-driven fluid flow in the zebrafish pronephros, brain and Kupffer's vesicle is required for normal organogenesis.
2005,
Pubmed Krock,
The intraflagellar transport protein IFT57 is required for cilia maintenance and regulates IFT-particle-kinesin-II dissociation in vertebrate photoreceptors.
2008,
Pubmed Lawo,
Subdiffraction imaging of centrosomes reveals higher-order organizational features of pericentriolar material.
2012,
Pubmed Lemullois,
Development and functions of the cytoskeleton during ciliogenesis in metazoa.
1988,
Pubmed Liu,
Notch signaling controls the differentiation of transporting epithelia and multiciliated cells in the zebrafish pronephros.
2007,
Pubmed Mennella,
Subdiffraction-resolution fluorescence microscopy reveals a domain of the centrosome critical for pericentriolar material organization.
2012,
Pubmed Meyer,
Gene and genome duplications in vertebrates: the one-to-four (-to-eight in fish) rule and the evolution of novel gene functions.
1999,
Pubmed Nakagawa,
Outer dense fiber 2 is a widespread centrosome scaffold component preferentially associated with mother centrioles: its identification from isolated centrosomes.
2001,
Pubmed Nigg,
The centrosome cycle: Centriole biogenesis, duplication and inherent asymmetries.
2011,
Pubmed Nigg,
Centrioles, centrosomes, and cilia in health and disease.
2009,
Pubmed Pearson,
Basal body assembly in ciliates: the power of numbers.
2009,
Pubmed Piperno,
Monoclonal antibodies specific for an acetylated form of alpha-tubulin recognize the antigen in cilia and flagella from a variety of organisms.
1985,
Pubmed
,
Xenbase Schermelleh,
A guide to super-resolution fluorescence microscopy.
2010,
Pubmed Schmidt,
Control of centriole length by CPAP and CP110.
2009,
Pubmed Shan,
Nudel and FAK as antagonizing strength modulators of nascent adhesions through paxillin.
2009,
Pubmed Showell,
Natural mating and tadpole husbandry in the western clawed frog Xenopus tropicalis.
2009,
Pubmed
,
Xenbase Sir,
A primary microcephaly protein complex forms a ring around parental centrioles.
2011,
Pubmed Sonnen,
3D-structured illumination microscopy provides novel insight into architecture of human centrosomes.
2012,
Pubmed Sorokin,
Reconstructions of centriole formation and ciliogenesis in mammalian lungs.
1968,
Pubmed Strnad,
Regulated HsSAS-6 levels ensure formation of a single procentriole per centriole during the centrosome duplication cycle.
2007,
Pubmed Sun,
Assembly of the FtsZ ring at the central division site in the absence of the chromosome.
1998,
Pubmed Tang,
The human microcephaly protein STIL interacts with CPAP and is required for procentriole formation.
2011,
Pubmed Tang,
Centriole biogenesis in multiciliated cells.
2013,
Pubmed Tang,
CPAP is a cell-cycle regulated protein that controls centriole length.
2009,
Pubmed Vladar,
Molecular characterization of centriole assembly in ciliated epithelial cells.
2007,
Pubmed Vulprecht,
STIL is required for centriole duplication in human cells.
2012,
Pubmed Wang,
The microtubule plus end-binding protein EB1 is involved in Sertoli cell plasticity in testicular seminiferous tubules.
2008,
Pubmed Wickner,
Posttranslational quality control: folding, refolding, and degrading proteins.
1999,
Pubmed Wu,
Nudel is crucial for the WAVE complex assembly in vivo by selectively promoting subcomplex stability and formation through direct interactions.
2012,
Pubmed You,
Growth and differentiation of mouse tracheal epithelial cells: selection of a proliferative population.
2002,
Pubmed Zardoya,
The complete DNA sequence of the mitochondrial genome of a "living fossil," the coelacanth (Latimeria chalumnae).
1997,
Pubmed Zeng,
The m subunit of murine translation initiation factor eIF3 maintains the integrity of the eIF3 complex and is required for embryonic development, homeostasis, and organ size control.
2013,
Pubmed Zhao,
Nephrocystins and MKS proteins interact with IFT particle and facilitate transport of selected ciliary cargos.
2011,
Pubmed
