Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Up-regulation of Kv1.3 channels by janus kinase 2.
Hosseinzadeh Z, Warsi J, Elvira B, Almilaji A, Shumilina E, Lang F.
???displayArticle.abstract???
The janus-activated kinase 2 JAK2 participates in the signalling of several hormones including interferon, a powerful regulator of lymphocyte function. Lymphocyte activity and survival depend on the activity of the voltage-gated K(+) channel KCNA3 (Kv1.3). The present study thus explored whether JAK2 modifies the activity of voltage-gated K(+) channel KCNA3. To this end, cRNA encoding KCNA3 was injected in Xenopus oocytes with or without additional injection of cRNA encoding wild-type human JAK2, human inactive (K882E)JAK2 mutant, or human gain-of-function (V617F)JAK2 mutant. KCNA3-dependent depolarization-induced current was determined utilizing dual-electrode voltage clamp, and protein KCNA3 abundance in the cell membrane was quantified by chemiluminescence. Moreover, the effect of interferon-γ on voltage-gated K(+) current was determined by patch clamp in mainly KCNA3-expressing Jurkat T cells with or without prior treatment with JAK2 inhibitor AG490 (40 µM). As a result, KCNA3 channel activity and protein abundance were up-regulated by coexpression of JAK2 or (V617F)JAK2 but not (K882E)JAK2. The effect of JAK2 coexpression was reversed by AG490 treatment. In human Jurkat T lymphoma cells, voltage-gated K(+) current was up-regulated by interferon-γ and down-regulated by AG490 (40 µM). In conclusion, JAK2 participates in the signalling, regulating the voltage-gated K(+) channel KCNA3.
Alesutan,
Upregulation of Na-coupled glucose transporter SGLT1 by Tau tubulin kinase 2.
2012, Pubmed,
Xenbase
Alesutan,
Upregulation of Na-coupled glucose transporter SGLT1 by Tau tubulin kinase 2.
2012,
Pubmed
,
Xenbase Almilaji,
Upregulation of Na+,Cl(-)-coupled betaine/γ-amino-butyric acid transporter BGT1 by Tau tubulin kinase 2.
2013,
Pubmed
,
Xenbase Almilaji,
Down-regulation of Na/K+ atpase activity by human parvovirus B19 capsid protein VP1.
2013,
Pubmed
,
Xenbase Arcangeli,
Targeting ion channels in leukemias: a new challenge for treatment.
2012,
Pubmed Axtell,
Janus-like effects of type I interferon in autoimmune diseases.
2012,
Pubmed Barry,
Liquid junction potentials and small cell effects in patch-clamp analysis.
1991,
Pubmed Bogatikov,
Up-regulation of amino acid transporter SLC6A19 activity and surface protein abundance by PKB/Akt and PIKfyve.
2012,
Pubmed
,
Xenbase Brooks,
The growth hormone receptor: mechanism of activation and clinical implications.
2010,
Pubmed Cahalan,
The functional network of ion channels in T lymphocytes.
2009,
Pubmed Choi,
Kv1.3: a potential pharmacological target for diabetes.
2010,
Pubmed Comes,
The voltage-dependent K(+) channels Kv1.3 and Kv1.5 in human cancer.
2013,
Pubmed Hamill,
Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches.
1981,
Pubmed Henke,
Regulation of the voltage gated K+ channel Kv1.3 by the ubiquitin ligase Nedd4-2 and the serum and glucocorticoid inducible kinase SGK1.
2004,
Pubmed
,
Xenbase Henrion,
Overlapping cardiac phenotype associated with a familial mutation in the voltage sensor of the KCNQ1 channel.
2012,
Pubmed
,
Xenbase Ho,
JAK2 Translocations in hematological malignancies: Review of the literature.
2010,
Pubmed Hosseinzadeh,
Downregulation of KCNQ4 by Janus kinase 2.
2013,
Pubmed
,
Xenbase Hosseinzadeh,
Down-regulation of the epithelial Na⁺ channel ENaC by Janus kinase 2.
2014,
Pubmed
,
Xenbase Hosseinzadeh,
Downregulation of ClC-2 by JAK2.
2012,
Pubmed
,
Xenbase Hosseinzadeh,
Down-regulation of the myoinositol transporter SMIT by JAK2.
2012,
Pubmed
,
Xenbase Hosseinzadeh,
Upregulation of peptide transporters PEPT1 and PEPT2 by Janus kinase JAK2.
2013,
Pubmed
,
Xenbase Jan,
Voltage-gated potassium channels and the diversity of electrical signalling.
2012,
Pubmed Johnson,
Gamma interferon signaling: insights to development of interferon mimetics.
2006,
Pubmed Ko,
Pathophysiology of voltage-gated K+ channels in vascular smooth muscle cells: modulation by protein kinases.
2010,
Pubmed Lang,
Cell volume regulatory ion channels in cell proliferation and cell death.
2007,
Pubmed Leanza,
Intracellular ion channels and cancer.
2013,
Pubmed Lopez,
Molecular basis of cytokine receptor activation.
2010,
Pubmed Mahaut-Smith,
The unique contribution of ion channels to platelet and megakaryocyte function.
2012,
Pubmed Mahfouz,
JAK2 V617F gene mutation in the laboratory work-up of myeloproliferative disorders: experience of a major referral center in Lebanon.
2011,
Pubmed Mia,
Downregulation of Kv1.5 K channels by the AMP-activated protein kinase.
2012,
Pubmed
,
Xenbase Morris,
Recent advances in understanding leptin signaling and leptin resistance.
2009,
Pubmed Munoz,
Up-regulation of the inwardly rectifying K⁺ channel Kir2.1 (KCNJ2) by protein kinase B (PKB/Akt) and PIKfyve.
2013,
Pubmed
,
Xenbase Noon-Song,
Controlling nuclear JAKs and STATs for specific gene activation by IFNγ.
2011,
Pubmed Oh,
JAK2 V617F and beyond: role of genetics and aberrant signaling in the pathogenesis of myeloproliferative neoplasms.
2010,
Pubmed Ouadid-Ahidouch,
K+ channel expression in human breast cancer cells: involvement in cell cycle regulation and carcinogenesis.
2008,
Pubmed Pakladok,
Upregulation of the Na⁺-coupled phosphate cotransporters NaPi-IIa and NaPi-IIb by B-RAF.
2014,
Pubmed
,
Xenbase Pakladok,
PIKfyve sensitivity of hERG channels.
2013,
Pubmed
,
Xenbase Panyi,
K+ channel blockers: novel tools to inhibit T cell activation leading to specific immunosuppression.
2006,
Pubmed Pardanani,
JAK inhibitor therapy for myelofibrosis: critical assessment of value and limitations.
2011,
Pubmed Pathare,
OSR1-sensitive renal tubular phosphate reabsorption.
2012,
Pubmed
,
Xenbase Pongs,
Ins and outs of cardiac voltage-gated potassium channels.
2009,
Pubmed Santos,
JAK2 inhibitors: what's the true therapeutic potential?
2011,
Pubmed Schmid,
SGK3 regulates Ca(2+) entry and migration of dendritic cells.
2012,
Pubmed Shen,
JAK2V617F/STAT5 signaling pathway promotes cell proliferation through activation of Pituitary Tumor Transforming Gene 1 expression.
2010,
Pubmed Sontheimer,
An unexpected role for ion channels in brain tumor metastasis.
2008,
Pubmed Spivak,
Narrative review: Thrombocytosis, polycythemia vera, and JAK2 mutations: The phenotypic mimicry of chronic myeloproliferation.
2010,
Pubmed Stackaruk,
Type I interferon regulation of natural killer cell function in primary and secondary infections.
2013,
Pubmed Szabò,
Contribution of voltage-gated potassium channels to the regulation of apoptosis.
2010,
Pubmed Szteyn,
Expression and functional significance of the Ca(2+)-activated Cl(-) channel ANO6 in dendritic cells.
2012,
Pubmed Tefferi,
Novel mutations and their functional and clinical relevance in myeloproliferative neoplasms: JAK2, MPL, TET2, ASXL1, CBL, IDH and IKZF1.
2010,
Pubmed Vacher,
Diverse roles for auxiliary subunits in phosphorylation-dependent regulation of mammalian brain voltage-gated potassium channels.
2011,
Pubmed Varga,
Ion channels in T lymphocytes: an update on facts, mechanisms and therapeutic targeting in autoimmune diseases.
2010,
Pubmed Venkitachalam,
Nuclear localization of lymphocyte-specific protein tyrosine kinase (Lck) and its role in regulating LIM domain only 2 (Lmo2) gene.
2012,
Pubmed Villalonga,
Potassium channels are a new target field in anticancer drug design.
2007,
Pubmed Wallace,
The life and death of breast cancer cells: proposing a role for the effects of phytoestrogens on potassium channels.
2011,
Pubmed Wang,
Targeting potassium channels Kv1.3 and KC a 3.1: routes to selective immunomodulators in autoimmune disorder treatment?
2013,
Pubmed Warsi,
Effect of Janus kinase 3 on the peptide transporters PEPT1 and PEPT2.
2013,
Pubmed
,
Xenbase Warsi,
Downregulation of chloride channel ClC-2 by Janus kinase 3.
2014,
Pubmed
,
Xenbase Wulff,
Targeting effector memory T-cells with Kv1.3 blockers.
2007,
Pubmed Xia,
Multiple regulatory sites in large-conductance calcium-activated potassium channels.
2002,
Pubmed Yang,
Growth hormone receptor targeting to lipid rafts requires extracellular subdomain 2.
2010,
Pubmed Yao,
Heme controls the regulation of protein tyrosine kinases Jak2 and Src.
2010,
Pubmed Zheng,
Interferon-λs: special immunomodulatory agents and potential therapeutic targets.
2013,
Pubmed
