Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Skalli O, Ropraz P, Trzeciak A, Benzonana G, Gillessen D, Gabbiani G.
???displayArticle.abstract???
A monoclonal antibody (anti-alpha sm-1) recognizing exclusively alpha-smooth muscle actin was selected and characterized after immunization of BALB/c mice with the NH2-terminal synthetic decapeptide of alpha-smooth muscle actin coupled to keyhole limpet hemocyanin. Anti-alpha sm-1 helped in distinguishing smooth muscle cells from fibroblasts in mixed cultures such as rat dermal fibroblasts and chicken embryo fibroblasts. In the aortic media, it recognized a hitherto unknown population of cells negative for alpha-smooth muscle actin and for desmin. In 5-d-old rats, this population is about half of the medial cells and becomes only 8 +/- 5% in 6-wk-old animals. In cultures of rat aortic media SMCs, there is a progressive increase of this cell population together with a progressive decrease in the number of alpha-smooth muscle actin-containing stress fibers per cell. Double immunofluorescent studies carried out with anti-alpha sm-1 and anti-desmin antibodies in several organs revealed a heterogeneity of stromal cells. Desmin-negative, alpha-smooth muscle actin-positive cells were found in the rat intestinal muscularis mucosae and in the dermis around hair follicles. Moreover, desmin-positive, alpha-smooth muscle actin-negative cells were identified in the intestinal submucosa, rat testis interstitium, and uterine stroma. alpha-Smooth muscle actin was also found in myoepithelial cells of mammary and salivary glands, which are known to express cytokeratins. Finally, alpha-smooth muscle actin is present in stromal cells of mammary carcinomas, previously considered fibroblastic in nature. Thus, anti-alpha sm-1 antibody appears to be a powerful probe in the study of smooth muscle differentiation in normal and pathological conditions.
Barja,
Actin isoform synthesis and mRNA levels in quiescent and proliferating rat aortic smooth muscle cells in vivo and in vitro.
1986, Pubmed
Barja,
Actin isoform synthesis and mRNA levels in quiescent and proliferating rat aortic smooth muscle cells in vivo and in vitro.
1986,
Pubmed Benyamin,
Anti-actin antibodies. Chemical modification allows the selective production of antibodies to the N-terminal region.
1986,
Pubmed Bulinski,
Peptide antibody specific for the amino terminus of skeletal muscle alpha-actin.
1983,
Pubmed Chaponnier,
Human plasma actin-depolymerizing factor. Purification, biological activity and localization in leukocytes and platelets.
1985,
Pubmed Franke,
Intermediate-sized filaments of the prekeratin type in myoepithelial cells.
1980,
Pubmed Franke,
Permanently proliferating rat vascular smooth muscle cell with maintained expression of smooth muscle characteristics, including actin of the vascular smooth muscle type.
1980,
Pubmed Gabbiani,
Vascular smooth muscle cells differ from other smooth muscle cells: predominance of vimentin filaments and a specific alpha-type actin.
1981,
Pubmed Gabbiani,
Granulation tissue as a contractile organ. A study of structure and function.
1972,
Pubmed Gabbiani,
Actin expression in smooth muscle cells of rat aortic intimal thickening, human atheromatous plaque, and cultured rat aortic media.
1984,
Pubmed Garrels,
Identification and characterization of multiple forms of actin.
1976,
Pubmed Gleiber,
Identification of a chemoattractant for fibroblasts produced by human breast carcinoma cell lines.
1984,
Pubmed Gown,
A smooth muscle-specific monoclonal antibody recognizes smooth muscle actin isozymes.
1985,
Pubmed Herman,
Microvascular pericytes contain muscle and nonmuscle actins.
1985,
Pubmed Hori,
Keratin type intermediate filaments in sweat gland myoepithelial cells.
1985,
Pubmed Ives,
Preparation of functional smooth muscle cells from the rabbit aorta.
1978,
Pubmed Keller,
Preparation and some properties of maleimido acids and maleoyl derivatives of peptides.
1975,
Pubmed Kocher,
Cytoskeletal features of normal and atheromatous human arterial smooth muscle cells.
1986,
Pubmed Kocher,
Cytoskeletal features of rat aortic cells during development. An electron microscopic, immunohistochemical, and biochemical study.
1985,
Pubmed Kocher,
Cytoskeleton of rat aortic smooth muscle cells. Normal conditions and experimental intimal thickening.
1984,
Pubmed Kuroda,
Change of actin isomers during differentiation of smooth muscle.
1985,
Pubmed Laemmli,
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
1970,
Pubmed Larson,
Heterogeneity of myosin antigenic expression in vascular smooth muscle in vivo.
1984,
Pubmed Leavitt,
Smooth muscle alpha-action is a transformation-sensitive marker for mouse NIH 3T3 and Rat-2 cells.
,
Pubmed Leavitt,
Dissimilar modes of expression of beta- and gamma-actin in normal and leukemic human T lymphocytes.
1980,
Pubmed Longtine,
Immunohistochemical localization of smooth muscle myosin in normal human tissues.
1985,
Pubmed Lubit,
Immunological characterization of an anti-actin antibody specific for cytoplasmic actins and its use for the immunocytological localization of actin in Aplysia nervous tissue.
1983,
Pubmed O'Farrell,
High resolution two-dimensional electrophoresis of proteins.
1975,
Pubmed Osborn,
Tumor diagnosis by intermediate filament typing: a novel tool for surgical pathology.
1983,
Pubmed Otey,
Immunolocalization of the gamma isoform of nonmuscle actin in cultured cells.
1986,
Pubmed Owens,
Expression of smooth muscle-specific alpha-isoactin in cultured vascular smooth muscle cells: relationship between growth and cytodifferentiation.
1986,
Pubmed Pardo,
Subcellular sorting of isoactins: selective association of gamma actin with skeletal muscle mitochondria.
1983,
Pubmed Rosenberg,
Isolation and characterization of actin and actin-binding protein from human platelets.
1981,
Pubmed Roustan,
Structural variations in actins. A study of the immunological reactivity of the N-terminal region.
1986,
Pubmed Rubenstein,
Differential behavior of gizzard isoactins.
1981,
Pubmed Rungger-Brändle,
The role of cytoskeletal and cytocontractile elements in pathologic processes.
1983,
Pubmed Saurat,
The intermediate filament proteins of rabbit normal epidermal Merkel cells are cytokeratins.
1984,
Pubmed Schmid,
Distribution of vimentin and desmin filaments in smooth muscle tissue of mammalian and avian aorta.
1982,
Pubmed Schürch,
Myofibroblastic stromal reaction in retracted scirrhous carcinoma of the breast.
1982,
Pubmed Simpson,
Monoclonal antibodies prepared against Dictyostelium actin: characterization and interactions with actin.
1984,
Pubmed Skalli,
Cytoskeletal remodeling of rat aortic smooth muscle cells in vitro: relationships to culture conditions and analogies to in vivo situations.
1986,
Pubmed Storti,
Chick cytoplasmic actin and muscle actin have different structural genes.
1976,
Pubmed Strauch,
Induction of vascular smooth muscle alpha-isoactin expression in BC3H1 cells.
1984,
Pubmed Takeuchi,
Differences between smooth and skeletal muscle myosins in their interactions with F-actin.
1982,
Pubmed Towbin,
Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.
1979,
Pubmed Travo,
Co-existence of vimentin and desmin type intermediate filaments in a subpopulation of adult rat vascular smooth muscle cells growing in primary culture.
1982,
Pubmed Tremblay,
Stromal aspects of breast carcinoma.
1979,
Pubmed Vandekerckhove,
Actin typing on total cellular extracts: a highly sensitive protein-chemical procedure able to distinguish different actins.
1981,
Pubmed Vandekerckhove,
The complete amino acid sequence of actins from bovine aorta, bovine heart, bovine fast skeletal muscle, and rabbit slow skeletal muscle. A protein-chemical analysis of muscle actin differentiation.
1979,
Pubmed Vandekerckhove,
At least six different actins are expressed in a higher mammal: an analysis based on the amino acid sequence of the amino-terminal tryptic peptide.
1978,
Pubmed Wehland,
Amino acid sequence requirements in the epitope recognized by the alpha-tubulin-specific rat monoclonal antibody YL 1/2.
1984,
Pubmed Whalen,
Protein synthesis and actin heterogeneity in calf muscle cells in culture.
1976,
Pubmed Witt,
Transformation-sensitive isoactin in passaged chick embryo fibroblasts transformed by Rous sarcoma virus.
1983,
Pubmed
