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Influence of oxidized purine processing on strand directionality of mismatch repair.
Repmann S, Olivera-Harris M, Jiricny J.
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Replicative DNA polymerases are high fidelity enzymes that misincorporate nucleotides into nascent DNA with a frequency lower than [1/10(5)], and this precision is improved to about [1/10(7)] by their proofreading activity. Because this fidelity is insufficient to replicate most genomes without error, nature evolved postreplicative mismatch repair (MMR), which improves the fidelity of DNA replication by up to 3 orders of magnitude through correcting biosynthetic errors that escaped proofreading. MMR must be able to recognize non-Watson-Crick base pairs and excise the misincorporated nucleotides from the nascent DNA strand, which carries by definition the erroneous genetic information. In eukaryotes, MMR is believed to be directed to the nascent strand by preexisting discontinuities such as gaps between Okazaki fragments in the lagging strand or breaks in the leading strand generated by the mismatch-activated endonuclease of the MutL homologs PMS1 in yeast and PMS2 in vertebrates. We recently demonstrated that the eukaryotic MMR machinery can make use also of strand breaks arising during excision of uracils or ribonucleotides from DNA. We now show that intermediates of MutY homolog-dependent excision of adenines mispaired with 8-oxoguanine (G(O)) also act as MMR initiation sites in extracts of human cells or Xenopus laevis eggs. Unexpectedly, G(O)/C pairs were not processed in these extracts and failed to affect MMR directionality, but extracts supplemented with exogenous 8-oxoguanine DNA glycosylase (OGG1) did so. Because OGG1-mediated excision of G(O) might misdirect MMR to the template strand, our findings suggest that OGG1 activity might be inhibited during MMR.
Avkin,
Efficiency, specificity and DNA polymerase-dependence of translesion replication across the oxidative DNA lesion 8-oxoguanine in human cells.
2002, Pubmed
Avkin,
Efficiency, specificity and DNA polymerase-dependence of translesion replication across the oxidative DNA lesion 8-oxoguanine in human cells.
2002,
Pubmed Baerenfaller,
Characterization of the "mismatch repairosome" and its role in the processing of modified nucleosides in vitro.
2006,
Pubmed Boldogh,
hMYH cell cycle-dependent expression, subcellular localization and association with replication foci: evidence suggesting replication-coupled repair of adenine:8-oxoguanine mispairs.
2001,
Pubmed Brooks,
Mismatch repair involving localized DNA synthesis in extracts of Xenopus eggs.
1989,
Pubmed
,
Xenbase Burrows,
Oxidative Nucleobase Modifications Leading to Strand Scission.
1998,
Pubmed Collins,
Oxidative DNA damage, antioxidants, and cancer.
1999,
Pubmed Colussi,
The mammalian mismatch repair pathway removes DNA 8-oxodGMP incorporated from the oxidized dNTP pool.
2002,
Pubmed David,
Base-excision repair of oxidative DNA damage.
2007,
Pubmed Dherin,
Excision of oxidatively damaged DNA bases by the human alpha-hOgg1 protein and the polymorphic alpha-hOgg1(Ser326Cys) protein which is frequently found in human populations.
1999,
Pubmed Dianov,
Generation of single-nucleotide repair patches following excision of uracil residues from DNA.
1992,
Pubmed Dianov,
Mammalian base excision repair: the forgotten archangel.
2013,
Pubmed Dufner,
Mismatch recognition and DNA-dependent stimulation of the ATPase activity of hMutSalpha is abolished by a single mutation in the hMSH6 subunit.
2000,
Pubmed Einolf,
Fidelity of nucleotide insertion at 8-oxo-7,8-dihydroguanine by mammalian DNA polymerase delta. Steady-state and pre-steady-state kinetic analysis.
2001,
Pubmed Fang,
Human strand-specific mismatch repair occurs by a bidirectional mechanism similar to that of the bacterial reaction.
1993,
Pubmed Fortini,
8-Oxoguanine DNA damage: at the crossroad of alternative repair pathways.
2003,
Pubmed Frosina,
Two pathways for base excision repair in mammalian cells.
1996,
Pubmed Gedik,
Establishing the background level of base oxidation in human lymphocyte DNA: results of an interlaboratory validation study.
2005,
Pubmed Ghodgaonkar,
Ribonucleotides misincorporated into DNA act as strand-discrimination signals in eukaryotic mismatch repair.
2013,
Pubmed Gillespie,
Preparation and use of Xenopus egg extracts to study DNA replication and chromatin associated proteins.
2012,
Pubmed
,
Xenbase Gu,
Human MutY homolog, a DNA glycosylase involved in base excision repair, physically and functionally interacts with mismatch repair proteins human MutS homolog 2/human MutS homolog 6.
2002,
Pubmed Hashimoto,
Rad51 protects nascent DNA from Mre11-dependent degradation and promotes continuous DNA synthesis.
2010,
Pubmed
,
Xenbase Holmes,
Strand-specific mismatch correction in nuclear extracts of human and Drosophila melanogaster cell lines.
1990,
Pubmed Iaccarino,
hMSH2 and hMSH6 play distinct roles in mismatch binding and contribute differently to the ATPase activity of hMutSalpha.
1998,
Pubmed Jacobs,
DNA glycosylases: in DNA repair and beyond.
2012,
Pubmed Jiricny,
The multifaceted mismatch-repair system.
2006,
Pubmed Kadyrov,
Endonucleolytic function of MutLalpha in human mismatch repair.
2006,
Pubmed Klungland,
Second pathway for completion of human DNA base excision-repair: reconstitution with purified proteins and requirement for DNase IV (FEN1).
1997,
Pubmed Koi,
Human chromosome 3 corrects mismatch repair deficiency and microsatellite instability and reduces N-methyl-N'-nitro-N-nitrosoguanidine tolerance in colon tumor cells with homozygous hMLH1 mutation.
1994,
Pubmed Kubota,
Determination of initiation of DNA replication before and after nuclear formation in Xenopus egg cell free extracts.
1993,
Pubmed
,
Xenbase Lahue,
Methyl-directed DNA mismatch repair in Escherichia coli.
1988,
Pubmed Larson,
Strand-specific processing of 8-oxoguanine by the human mismatch repair pathway: inefficient removal of 8-oxoguanine paired with adenine or cytosine.
2003,
Pubmed Maki,
MutT protein specifically hydrolyses a potent mutagenic substrate for DNA synthesis.
1992,
Pubmed Mazurek,
Activation of human MutS homologs by 8-oxo-guanine DNA damage.
2002,
Pubmed McAuley-Hecht,
Crystal structure of a DNA duplex containing 8-hydroxydeoxyguanine-adenine base pairs.
1994,
Pubmed Michaels,
The GO system protects organisms from the mutagenic effect of the spontaneous lesion 8-hydroxyguanine (7,8-dihydro-8-oxoguanine).
1992,
Pubmed Mo,
Hydrolytic elimination of a mutagenic nucleotide, 8-oxodGTP, by human 18-kilodalton protein: sanitization of nucleotide pool.
1992,
Pubmed Modrich,
Mechanisms in eukaryotic mismatch repair.
2006,
Pubmed Modrich,
Mismatch repair in replication fidelity, genetic recombination, and cancer biology.
1996,
Pubmed Nash,
Cloning of a yeast 8-oxoguanine DNA glycosylase reveals the existence of a base-excision DNA-repair protein superfamily.
1996,
Pubmed Neuberger,
Somatic hypermutation at A.T pairs: polymerase error versus dUTP incorporation.
2005,
Pubmed Ohtsubo,
Identification of human MutY homolog (hMYH) as a repair enzyme for 2-hydroxyadenine in DNA and detection of multiple forms of hMYH located in nuclei and mitochondria.
2000,
Pubmed Peña-Diaz,
PCNA and MutLα: partners in crime in triplet repeat expansion?
2010,
Pubmed Peña-Diaz,
Noncanonical mismatch repair as a source of genomic instability in human cells.
2012,
Pubmed Pluciennik,
PCNA function in the activation and strand direction of MutLα endonuclease in mismatch repair.
2010,
Pubmed Russo,
The oxidized deoxynucleoside triphosphate pool is a significant contributor to genetic instability in mismatch repair-deficient cells.
2004,
Pubmed Sakumi,
Cloning and expression of cDNA for a human enzyme that hydrolyzes 8-oxo-dGTP, a mutagenic substrate for DNA synthesis.
1993,
Pubmed Schanz,
Interference of mismatch and base excision repair during the processing of adjacent U/G mispairs may play a key role in somatic hypermutation.
2009,
Pubmed Shibutani,
Insertion of specific bases during DNA synthesis past the oxidation-damaged base 8-oxodG.
1991,
Pubmed Thomas,
Heteroduplex repair in extracts of human HeLa cells.
1991,
Pubmed Ushijima,
A functional analysis of the DNA glycosylase activity of mouse MUTYH protein excising 2-hydroxyadenine opposite guanine in DNA.
2005,
Pubmed van Loon,
An 8-oxo-guanine repair pathway coordinated by MUTYH glycosylase and DNA polymerase lambda.
2009,
Pubmed Warren,
Structure of the human MutSalpha DNA lesion recognition complex.
2007,
Pubmed
