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Although Mϕ represent the most primordial immune cell subsets, the mechanisms governing their functional heterogeneity remain poorly defined. However, it is well established that the CSF-1 cytokine contributes to monopoiesis and to this heterogeneity, whereas the unrelated IL-34 also binds the CSF-1R toward poorly understood immunologic roles. To delineate the molecular and evolutionary basis behind vertebrate Mϕ functional heterogeneity, we performed comprehensive transcriptional and functional studies of amphibian (Xenopus laevis) BM (in vitro) and PER (in vivo) Mϕ derived by rXlCSF-1 and rXlIL-34. Our findings indicate that these amphibian cytokines promote morphologically and functionally distinct Mϕ populations. Mϕ induced by rXlCSF-1 possess more robust iNOS gene expression, are substantially more phagocytic, display greater NO responses, and exhibit enhanced bactericidal capacities. By contrast, rXlIL-34-derived Mϕ express greater levels of Arg-1 and NADPH oxidase components and possess greater respiratory burst responses. Most notably, whereas CSF-1 Mϕ are highly susceptible to the emerging FV3 ranavirus, rXlIL-34 Mϕ exhibit potent antiviral activity against this pathogen, which is dependent on reactive oxygen production. This work marks an advance in our understanding of the possible mechanisms governing vertebrate Mϕ functional heterogeneity.
Belosevic,
Development of goldfish macrophages in vitro.
2006, Pubmed
Belosevic,
Development of goldfish macrophages in vitro.
2006,
Pubmed Chen,
Intraembryonic origin of hepatic hematopoiesis in Xenopus laevis.
1995,
Pubmed
,
Xenbase Chinchar,
Ranaviruses (family Iridoviridae): emerging cold-blooded killers.
2002,
Pubmed De Jesús Andino,
Susceptibility of Xenopus laevis tadpoles to infection by the ranavirus Frog-Virus 3 correlates with a reduced and delayed innate immune response in comparison with adult frogs.
2012,
Pubmed
,
Xenbase De Kleer,
Ontogeny of myeloid cells.
2014,
Pubmed Droin,
Editorial: CSF1R, CSF-1, and IL-34, a "menage a trois" conserved across vertebrates.
2010,
Pubmed Garceau,
Pivotal Advance: Avian colony-stimulating factor 1 (CSF-1), interleukin-34 (IL-34), and CSF-1 receptor genes and gene products.
2010,
Pubmed Gordon,
Alternative activation of macrophages: mechanism and functions.
2010,
Pubmed Gordon,
Monocyte and macrophage heterogeneity.
2005,
Pubmed Grayfer,
Divergent antiviral roles of amphibian (Xenopus laevis) macrophages elicited by colony-stimulating factor-1 and interleukin-34.
2014,
Pubmed
,
Xenbase Grayfer,
Colony-stimulating factor-1-responsive macrophage precursors reside in the amphibian (Xenopus laevis) bone marrow rather than the hematopoietic subcapsular liver.
2013,
Pubmed
,
Xenbase Grayfer,
The amphibian (Xenopus laevis) type I interferon response to frog virus 3: new insight into ranavirus pathogenicity.
2014,
Pubmed
,
Xenbase Guilbert,
Specific interaction of murine colony-stimulating factor with mononuclear phagocytic cells.
1980,
Pubmed Hadji-Azimi,
Atlas of adult Xenopus laevis laevis hematology.
1987,
Pubmed
,
Xenbase Hadji-Azimi,
B-lymphocyte populations in Xenopus laevis.
1990,
Pubmed
,
Xenbase Hamilton,
Myeloid colony-stimulating factors as regulators of macrophage polarization.
2014,
Pubmed Hanington,
Growth factors of lower vertebrates: characterization of goldfish (Carassius auratus L.) macrophage colony-stimulating factor-1.
2007,
Pubmed Kim,
Reactive oxygen species induce antiviral innate immune response through IFN-λ regulation in human nasal epithelial cells.
2013,
Pubmed Lichanska,
Differentiation of the mononuclear phagocyte system during mouse embryogenesis: the role of transcription factor PU.1.
1999,
Pubmed Lin,
Discovery of a cytokine and its receptor by functional screening of the extracellular proteome.
2008,
Pubmed Lupfer,
The expanding role of NLRs in antiviral immunity.
2013,
Pubmed Mantovani,
Macrophage polarization: tumor-associated macrophages as a paradigm for polarized M2 mononuclear phagocytes.
2002,
Pubmed Mills,
M1 and M2 Macrophages: Oracles of Health and Disease.
2012,
Pubmed Molteni,
Reactive oxygen and nitrogen species during viral infections.
2014,
Pubmed Morales,
Innate immune responses and permissiveness to ranavirus infection of peritoneal leukocytes in the frog Xenopus laevis.
2010,
Pubmed
,
Xenbase Nogawa-Kosaka,
Identification of erythroid progenitors induced by erythropoietic activity in Xenopus laevis.
2011,
Pubmed
,
Xenbase Paterniti,
Protective effects of apocynin, an inhibitor of NADPH oxidase activity, in splanchnic artery occlusion and reperfusion.
2010,
Pubmed Pixley,
CSF-1 regulation of the wandering macrophage: complexity in action.
2004,
Pubmed Robert,
Xenopus laevis: a possible vector of Ranavirus infection?
2007,
Pubmed
,
Xenbase Secombes,
The interleukins of fish.
2011,
Pubmed Shen,
Determinants of eosinophil survival and apoptotic cell death.
2015,
Pubmed Strengert,
Mucosal reactive oxygen species are required for antiviral response: role of Duox in influenza a virus infection.
2014,
Pubmed Venkatesh,
Elephant shark genome provides unique insights into gnathostome evolution.
2014,
Pubmed Verreck,
Human IL-23-producing type 1 macrophages promote but IL-10-producing type 2 macrophages subvert immunity to (myco)bacteria.
2004,
Pubmed Vlahos,
Inhibition of Nox2 oxidase activity ameliorates influenza A virus-induced lung inflammation.
2011,
Pubmed Wang,
Molecular mechanisms that influence the macrophage m1-m2 polarization balance.
2014,
Pubmed Wang,
Two macrophage colony-stimulating factor genes exist in fish that differ in gene organization and are differentially expressed.
2008,
Pubmed Wei,
Functional overlap but differential expression of CSF-1 and IL-34 in their CSF-1 receptor-mediated regulation of myeloid cells.
2010,
Pubmed Ye,
Inhibition of reactive oxygen species production ameliorates inflammation induced by influenza A viruses via upregulation of SOCS1 and SOCS3.
2015,
Pubmed Zhou,
Macrophage polarization and function with emphasis on the evolving roles of coordinated regulation of cellular signaling pathways.
2014,
Pubmed
