Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Glycoconj J
2002 Mar 01;193:187-95. doi: 10.1023/A:1024201824354.
Show Gene links
Show Anatomy links
Two closely related forms of UDP-GlcNAc: alpha6-D-mannoside beta1,2-N-acetylglucosaminyltransferase II occur in the clawed frog Xenopus laevis.
Mucha J, Svoboda B, Kappel S, Strasser R, Bencur P, Fröhwein U, Schachter H, Mach L, Glössl J.
???displayArticle.abstract???
UDP-GlcNAc:alpha6-D-mannoside beta1,2-N-acetylglucosaminyltransferase II (GnT II; EC 2.4.1.143) is a medial-Golgi resident enzyme that catalyses an essential step in the biosynthetic pathway leading from high mannose to complex N-linked oligosaccharides. Screening a cDNA library from Xenopus laevis ovary with a human GnT II DNA probe resulted in the isolation of two cDNA clones encoding two closely related GnT II isoenzymes, GnT II-A and GnT II-B. Analysis of the corresponding genomic DNAs revealed that the open reading frame of both X. laevis GnT II genes resides within a single exon. The GnT II-A gene was found to be transcriptionally active in all X. laevis tissues tested. In contrast, expression of the GnT II-B gene was detected only in a limited number of tissues. Both GnT II-A and GnT II-B exhibit a type II transmembrane protein topology with a putative N-terminal cytoplasmic tail of 9 amino acids followed by a transmembrane domain of 18 residues, and a C-terminal luminal domain of 405 residues. The two proteins differ at 28 amino acid positions within their luminal regions. Heterologous expression of soluble forms of the enzymes in insect cells showed that GnT II-A and GnT II-B are both catalytically active and exhibit similar specific activities. Both recombinant proteins are modified with N-linked oligosaccharides. N-terminal deletion studies demonstrated that the first 49 amino acid residues are not essential for proper folding and enzymatic activity of X. laevis GnT II.
Altmann,
Determination of amino sugars and amino acids in glycoconjugates using precolumn derivatization with o-phthalaldehyde.
1992, Pubmed
Altmann,
Determination of amino sugars and amino acids in glycoconjugates using precolumn derivatization with o-phthalaldehyde.
1992,
Pubmed Altmann,
Processing of asparagine-linked oligosaccharides in insect cells. N-acetylglucosaminyltransferase I and II activities in cultured lepidopteran cells.
1993,
Pubmed Amaya,
Frog genetics: Xenopus tropicalis jumps into the future.
1998,
Pubmed
,
Xenbase Bisbee,
Albumin phylogeny for clawed frogs (Xenopus).
1977,
Pubmed
,
Xenbase Breton,
Structural and functional features of glycosyltransferases.
2001,
Pubmed Cantor,
A method for [3H]mannose labeling of Asn-linked oligosaccharides on recombinant glycoproteins synthesized in Xenopus oocytes.
1992,
Pubmed
,
Xenbase Charuk,
Carbohydrate-deficient glycoprotein syndrome type II. An autosomal recessive N-acetylglucosaminyltransferase II deficiency different from typical hereditary erythroblastic multinuclearity, with a positive acidified-serum lysis test (HEMPAS).
1995,
Pubmed Chen,
UDP-N-acetylglucosamine:alpha-3-D-mannoside beta-1,2-N-acetylglucosaminyltransferase I and UDP-N-acetylglucosamine:alpha-6-D-mannoside beta-1,2-N-acetylglucosaminyltransferase II in Caenorhabditis elegans.
2002,
Pubmed Chomczynski,
Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction.
1987,
Pubmed D'Agostaro,
Molecular cloning and expression of cDNA encoding the rat UDP-N-acetylglucosamine:alpha-6-D-mannoside beta-1,2-N-acetylglucosaminyltransferase II.
1995,
Pubmed Jaeken,
Carbohydrate deficient glycoprotein syndrome type II: a deficiency in Golgi localised N-acetyl-glucosaminyltransferase II.
1994,
Pubmed Khanna,
Glycosylation increases potassium channel stability and surface expression in mammalian cells.
2001,
Pubmed
,
Xenbase Kornfeld,
Assembly of asparagine-linked oligosaccharides.
1985,
Pubmed Kyte,
A simple method for displaying the hydropathic character of a protein.
1982,
Pubmed Leeb,
Molecular cloning of the porcine beta-1,2-N-acetylglucosaminyltransferase II gene and assignment to chromosome 1q23-q27.
1997,
Pubmed Mucha,
Tissues of the clawed frog Xenopus laevis contain two closely related forms of UDP-GlcNAc:alpha3-D-mannoside beta-1,2-N-acetylglucosaminyltransferase I.
2001,
Pubmed
,
Xenbase Roitsch,
Expression of yeast invertase in oocytes from Xenopus laevis. Secretion of active enzyme differing in glycosylation.
1989,
Pubmed
,
Xenbase Sarkar,
Removal of 106 amino acids from the N-terminus of UDP-GlcNAc: alpha-3-D-mannoside beta-1,2-N-acetylglucosaminyltransferase I does not inactivate the enzyme.
1998,
Pubmed Schachter,
Biosynthetic controls that determine the branching and microheterogeneity of protein-bound oligosaccharides.
1986,
Pubmed Schachter,
Carbohydrate-deficient glycoprotein syndrome type II.
1999,
Pubmed Strasser,
Molecular cloning of cDNA encoding N-acetylglucosaminyltransferase II from Arabidopsis thaliana.
1999,
Pubmed Tan,
The human UDP-N-acetylglucosamine: alpha-6-D-mannoside-beta-1,2- N-acetylglucosaminyltransferase II gene (MGAT2). Cloning of genomic DNA, localization to chromosome 14q21, expression in insect cells and purification of the recombinant protein.
1995,
Pubmed Tan,
Mutations in the MGAT2 gene controlling complex N-glycan synthesis cause carbohydrate-deficient glycoprotein syndrome type II, an autosomal recessive disease with defective brain development.
1996,
Pubmed Unligil,
X-ray crystal structure of rabbit N-acetylglucosaminyltransferase I: catalytic mechanism and a new protein superfamily.
2000,
Pubmed Wang,
Modeling human congenital disorder of glycosylation type IIa in the mouse: conservation of asparagine-linked glycan-dependent functions in mammalian physiology and insights into disease pathogenesis.
2001,
Pubmed Wiggins,
Activity of the yeast MNN1 alpha-1,3-mannosyltransferase requires a motif conserved in many other families of glycosyltransferases.
1998,
Pubmed
