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Parasitol Res
2016 Jan 01;1151:347-54. doi: 10.1007/s00436-015-4754-y.
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Molecular cloning and functional characterization of a glucose transporter (CsGLUT) in Clonorchis sinensis.
Ahn SK, Cho PY, Na BK, Hong SJ, Nam HW, Sohn WM, Ardelli BF, Park YK, Kim TS, Cha SH.
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A complementary DNA (cDNA) encoding a glucose transporter of Clonorchis sinensis (CsGLUT) was isolated from the adult C. sinensis cDNA library. The open reading frame of CsGLUT cDNA consists of 1653 base pairs that encode a 550-amino acid residue protein. Hydropathy analysis suggested that CsGLUT possess 12 putative membrane-spanning domains. The Northern blot analysis result using poly(A)(+)RNA showed a strong band at ~2.1 kb for CsGLUT. When expressed in Xenopus oocytes, CsGLUT mediated the transport of radiolabeled deoxy-D-glucose in a time-dependent but sodium-independent manner. Concentration-dependency results showed saturable kinetics and followed the Michaelis-Menten equation. Nonlinear regression analyses yielded a Km value of 588.5 ± 53.0 μM and a Vmax value of 1500.0 ± 67.5 pmol/oocyte/30 min for [1,2-(3)H]2-deoxy-D-glucose. No trans-uptakes of bile acid (taurocholic acid), amino acids (tryptophan and arginine), or p-aminohippuric acid were observed. CsGLUT-mediated transport of deoxyglucose was significantly and concentration-dependently inhibited by radio-unlabeled deoxyglucose and D-glucose. 3-O-Methylglucose at 10 and 100 μM inhibited deoxyglucose uptake by ~50 % without concentration dependence. No inhibitory effects by galactose, mannose, and fructose were observed. This work may contribute to the molecular biological study of carbohydrate metabolism and new drug development of C. sinensis.
Baldwin,
Homologies between sugar transporters from eukaryotes and prokaryotes.
1989, Pubmed
Baldwin,
Homologies between sugar transporters from eukaryotes and prokaryotes.
1989,
Pubmed Bell,
Molecular biology of mammalian glucose transporters.
1990,
Pubmed Carruthers,
Facilitated diffusion of glucose.
1990,
Pubmed Carruthers,
Will the original glucose transporter isoform please stand up!
2009,
Pubmed Cha,
Molecular cloning and characterization of multispecific organic anion transporter 4 expressed in the placenta.
2000,
Pubmed
,
Xenbase Chen,
Epidemiological investigation of Clonorchis sinensis infection in freshwater fishes in the Pearl River Delta.
2010,
Pubmed Choi,
Clonorchiasis and cholangiocarcinoma: etiologic relationship and imaging diagnosis.
2004,
Pubmed Cornford,
Tegumental glucose permeability in male and female Schistosoma mansoni.
1988,
Pubmed Cornford,
Transintegumental glucose uptake in Schistosomatium douthitti.
1981,
Pubmed Fried,
Helminths in human carcinogenesis.
2011,
Pubmed Henderson,
Homologous sugar transport proteins in Escherichia coli and their relatives in both prokaryotes and eukaryotes.
1990,
Pubmed Isseroff,
Monosaccharide absorption by Schistosoma mansoni. I. Kinetic characteristics.
1972,
Pubmed Joost,
Structure-function relationship of glucose transporters catalyzing facilitated diffusion.
1994,
Pubmed Kang,
Study on the (14)C-glucose metabolism by Clonorchis sinensis: Paper Chromatographic Analyses in Combination with Autoradiography.
1969,
Pubmed Lee,
Cloning of the gene for phosphoglycerate kinase from Schistosoma mansoni and characterization of its gene product.
1995,
Pubmed Lim,
Clonorchis sinensis infection and increasing risk of cholangiocarcinoma in the Republic of Korea.
2006,
Pubmed Lun,
Clonorchiasis: a key foodborne zoonosis in China.
2005,
Pubmed Podesta,
A method for determining nonelectrolyte uptake by adult schistosoma mansoni using compartmental analysis of hexose fluxes.
1982,
Pubmed Rim,
Clonorchiasis: an update.
2005,
Pubmed Scheepers,
The glucose transporter families SGLT and GLUT: molecular basis of normal and aberrant function.
2004,
Pubmed Simpson,
The facilitative glucose transporter GLUT3: 20 years of distinction.
2008,
Pubmed Skelly,
Rapid appearance and asymmetric distribution of glucose transporter SGTP4 at the apical surface of intramammalian-stage Schistosoma mansoni.
1996,
Pubmed Skelly,
Cloning, characterization, and functional expression of cDNAs encoding glucose transporter proteins from the human parasite Schistosoma mansoni.
1994,
Pubmed
,
Xenbase Skelly,
Glucose Transport and Metabolism in Mammalian-stage Schistosomes.
1998,
Pubmed Sripa,
Liver fluke induces cholangiocarcinoma.
2007,
Pubmed Tamura,
MEGA6: Molecular Evolutionary Genetics Analysis version 6.0.
2013,
Pubmed Uglem,
Sugar transport and metabolism in Schistosoma mansoni.
1975,
Pubmed Zhang,
Epidemiological study on Clonorchis sinensis infection in Shenzhen area of Zhujiang delta in China.
2007,
Pubmed
