Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Distribution of single wall carbon nanotubes in the Xenopus laevis embryo after microinjection.
Holt BD, Shawky JH, Dahl KN, Davidson LA, Islam MF.
???displayArticle.abstract???
Single wall carbon nanotubes (SWCNTs) are advanced materials with the potential for a myriad of diverse applications, including biological technologies and large-scale usage with the potential for environmental impacts. SWCNTs have been exposed to developing organisms to determine their effects on embryogenesis, and results have been inconsistent arising, in part, from differing material quality, dispersion status, material size, impurity from catalysts and stability. For this study, we utilized highly purified SWCNT samples with short, uniform lengths (145 ± 17 nm) well dispersed in solution. To test high exposure doses, we microinjected > 500 µg ml(-1) SWCNT concentrations into the well-established embryogenesis model, Xenopus laevis, and determined embryo compatibility and subcellular localization during development. SWCNTs localized within cellular progeny of the microinjected cells, but were heterogeneously distributed throughout the target-injected tissue. Co-registering unique Raman spectral intensity of SWCNTs with images of fluorescently labeled subcellular compartments demonstrated that even at regions of highest SWCNT concentration, there were no gross alterations to subcellular microstructures, including filamentous actin, endoplasmic reticulum and vesicles. Furthermore, SWCNTs did not aggregate and localized to the perinuclear subcellular region. Combined, these results suggest that purified and dispersed SWCNTs are not toxic to X. laevis animal cap ectoderm and may be suitable candidate materials for biological applications.
Arnold,
Sorting carbon nanotubes by electronic structure using density differentiation.
2006, Pubmed
Arnold,
Sorting carbon nanotubes by electronic structure using density differentiation.
2006,
Pubmed Bacchetta,
Nano-sized CuO, TiO₂ and ZnO affect Xenopus laevis development.
2012,
Pubmed
,
Xenbase Bachilo,
Structure-assigned optical spectra of single-walled carbon nanotubes.
2002,
Pubmed Battigelli,
Endowing carbon nanotubes with biological and biomedical properties by chemical modifications.
2013,
Pubmed Bridges,
Comparative contaminant toxicity: are amphibian larvae more sensitive than fish?
2002,
Pubmed Campagnolo,
Biodistribution and toxicity of pegylated single wall carbon nanotubes in pregnant mice.
2013,
Pubmed Campagnolo,
Physico-chemical properties mediating reproductive and developmental toxicity of engineered nanomaterials.
2012,
Pubmed Chen,
Molecular characterization of toxicity mechanism of single-walled carbon nanotubes.
2013,
Pubmed Cheng,
Acute and long-term effects after single loading of functionalized multi-walled carbon nanotubes into zebrafish (Danio rerio).
2009,
Pubmed Cheng,
Influence of carbon nanotube length on toxicity to zebrafish embryos.
2012,
Pubmed Cheng,
Effect of carbon nanotubes on developing zebrafish (Danio rerio) embryos.
2007,
Pubmed Cheng,
Nanotherapeutics in angiogenesis: synthesis and in vivo assessment of drug efficacy and biocompatibility in zebrafish embryos.
2011,
Pubmed Curry,
Fatty acid binding to human serum albumin: new insights from crystallographic studies.
1999,
Pubmed Dubertret,
In vivo imaging of quantum dots encapsulated in phospholipid micelles.
2002,
Pubmed
,
Xenbase Dulińska-Molak,
Variation of mechanical property of single-walled carbon nanotubes-treated cells explored by atomic force microscopy.
2014,
Pubmed Giannaccini,
Non-mammalian vertebrate embryos as models in nanomedicine.
2014,
Pubmed
,
Xenbase Gimlich,
Improved fluorescent compounds for tracing cell lineage.
1985,
Pubmed
,
Xenbase Hamdoun,
Embryo stability and vulnerability in an always changing world.
2007,
Pubmed Heister,
Are carbon nanotubes a natural solution? Applications in biology and medicine.
2013,
Pubmed Hersam,
Progress towards monodisperse single-walled carbon nanotubes.
2008,
Pubmed Holt,
Not all protein-mediated single-wall carbon nanotube dispersions are equally bioactive.
2012,
Pubmed Holt,
Altered cell mechanics from the inside: dispersed single wall carbon nanotubes integrate with and restructure actin.
2012,
Pubmed Holt,
Differential sub-cellular processing of single-wall carbon nanotubes via interfacial modifications.
2015,
Pubmed Holt,
Cells take up and recover from protein-stabilized single-wall carbon nanotubes with two distinct rates.
2012,
Pubmed Holt,
Quantification of uptake and localization of bovine serum albumin-stabilized single-wall carbon nanotubes in different human cell types.
2011,
Pubmed Holt,
Carbon nanotubes reorganize actin structures in cells and ex vivo.
2010,
Pubmed Hong,
Multifunctional in vivo vascular imaging using near-infrared II fluorescence.
2012,
Pubmed Hougaard,
Effects of lung exposure to carbon nanotubes on female fertility and pregnancy. A study in mice.
2013,
Pubmed Hough,
Viscoelasticity of single wall carbon nanotube suspensions.
2004,
Pubmed Hough,
Structure of semidilute single-wall carbon nanotube suspensions and gels.
2006,
Pubmed Huang,
The genotype-dependent influence of functionalized multiwalled carbon nanotubes on fetal development.
2014,
Pubmed Jackson,
Bioaccumulation and ecotoxicity of carbon nanotubes.
2013,
Pubmed Jackson,
Exposure of pregnant mice to carbon black by intratracheal instillation: toxicogenomic effects in dams and offspring.
2012,
Pubmed Jariwala,
Carbon nanomaterials for electronics, optoelectronics, photovoltaics, and sensing.
2013,
Pubmed Jin,
Size-dependent cellular uptake and expulsion of single-walled carbon nanotubes: single particle tracking and a generic uptake model for nanoparticles.
2009,
Pubmed Jin,
Single-particle tracking of endocytosis and exocytosis of single-walled carbon nanotubes in NIH-3T3 cells.
2008,
Pubmed Johnston,
Electronic devices based on purified carbon nanotubes grown by high-pressure decomposition of carbon monoxide.
2005,
Pubmed Kagan,
Direct and indirect effects of single walled carbon nanotubes on RAW 264.7 macrophages: role of iron.
2006,
Pubmed Kaiser,
Single walled carbon nanotubes (SWCNT) affect cell physiology and cell architecture.
2008,
Pubmed Kim,
Embryonic toxicity changes of organic nanomaterials in the presence of natural organic matter.
2012,
Pubmed Kolosnjaj-Tabi,
In vivo behavior of large doses of ultrashort and full-length single-walled carbon nanotubes after oral and intraperitoneal administration to Swiss mice.
2010,
Pubmed Leeuw,
Single-walled carbon nanotubes in the intact organism: near-IR imaging and biocompatibility studies in Drosophila.
2007,
Pubmed Liu,
Drosophila embryos as model to assess cellular and developmental toxicity of multi-walled carbon nanotubes (MWCNT) in living organisms.
2014,
Pubmed Liu,
Inhibition of proliferation and differentiation of mesenchymal stem cells by carboxylated carbon nanotubes.
2010,
Pubmed Liu,
Carbon Nanotubes in Biology and Medicine: In vitro and in vivo Detection, Imaging and Drug Delivery.
2009,
Pubmed Mouchet,
Characterisation and in vivo ecotoxicity evaluation of double-wall carbon nanotubes in larvae of the amphibian Xenopus laevis.
2008,
Pubmed
,
Xenbase Mouchet,
International amphibian micronucleus standardized procedure (ISO 21427-1) for in vivo evaluation of double-walled carbon nanotubes toxicity and genotoxicity in water.
2011,
Pubmed
,
Xenbase Mouchet,
Carbon nanotube ecotoxicity in amphibians: assessment of multiwalled carbon nanotubes and comparison with double-walled carbon nanotubes.
2010,
Pubmed
,
Xenbase Murugesan,
Carbon inhibits vascular endothelial growth factor- and fibroblast growth factor-promoted angiogenesis.
2007,
Pubmed Nouara,
Carboxylic acid functionalization prevents the translocation of multi-walled carbon nanotubes at predicted environmentally relevant concentrations into targeted organs of nematode Caenorhabditis elegans.
2013,
Pubmed O'Connell,
Band gap fluorescence from individual single-walled carbon nanotubes.
2002,
Pubmed Pietroiusti,
Low doses of pristine and oxidized single-wall carbon nanotubes affect mammalian embryonic development.
2011,
Pubmed Rodriguez-Fernandez,
Multiwalled carbon nanotubes display microtubule biomimetic properties in vivo, enhancing microtubule assembly and stabilization.
2012,
Pubmed Roman,
Significant toxic role for single-walled carbon nanotubes during normal embryogenesis.
2013,
Pubmed Ruggiero,
Paradoxical glomerular filtration of carbon nanotubes.
2010,
Pubmed Sargent,
Single-walled carbon nanotube-induced mitotic disruption.
2012,
Pubmed Saria,
Short term exposure to multi-walled carbon nanotubes induce oxidative stress and DNA damage in Xenopus laevis tadpoles.
2014,
Pubmed
,
Xenbase Shams,
Actin reorganization through dynamic interactions with single-wall carbon nanotubes.
2014,
Pubmed Vankayala,
Effects of surface functionality of carbon nanomaterials on short-term cytotoxicity and embryonic development in zebrafish.
2014,
Pubmed Villegas,
Multiwalled carbon nanotubes hinder microglia function interfering with cell migration and phagocytosis.
2014,
Pubmed Welsher,
Deep-tissue anatomical imaging of mice using carbon nanotube fluorophores in the second near-infrared window.
2011,
Pubmed Wierzbicki,
Comparison of anti-angiogenic properties of pristine carbon nanoparticles.
2013,
Pubmed Yaron,
Single wall carbon nanotubes enter cells by endocytosis and not membrane penetration.
2011,
Pubmed Zhang,
Influences of acid-treated multiwalled carbon nanotubes on fibroblasts: proliferation, adhesion, migration, and wound healing.
2011,
Pubmed
