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Exploring the phosphoproteome profiles during Xenopus egg activation by calcium stimulation using a fully automated phosphopeptide purification system.
Kanno T, Furukawa K, Horigome T.
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To explore the phosphoproteome profiles duringXenopusegg activation by Ca(2+)-stimulation, an automated phosphopeptide purification system involving a titania column was improved by introducing 4-step elution with phosphate buffers. The number of detected phosphopeptides in the tryptic digest of aXenopusegg cytosol fraction on mass spectrometry (MS) was increased 1.5-fold and the percentage of multiply phosphorylated peptides increased from 17 to 24% with introduction of the 4-step elution method. Phosphopeptides were purified by the improved method from tryptic digests of cytosol fractions ofXenopuseggs without and with a Ca(2+)-stimulus, and then, analysed by MS. One thousand three hundred and seventy-five and 994 phosphopeptides were reproducibly detected on duplicate MS, respectively. They included 818 and 437 phosphopeptides specific to each digest, respectively. A method involving isobaric tags for relative and absolute quantitation (iTRAQ) was also applied to compare the phosphorylation levels inXenopuseggs without and with a Ca(2+)-stimulus, the ratios for 112 phosphopeptides in tryptic digests of these egg cytosol fractions being obtained. It was suggested from all the results that the phosphorylation sites and levels change duringXenopusegg activation for many known and unknown sites on structural proteins, signalling related proteins, cell cycle-related proteins and others.
Backs,
The gamma isoform of CaM kinase II controls mouse egg activation by regulating cell cycle resumption.
2010, Pubmed
Backs,
The gamma isoform of CaM kinase II controls mouse egg activation by regulating cell cycle resumption.
2010,
Pubmed Bidinosti,
Postnatal deamidation of 4E-BP2 in brain enhances its association with raptor and alters kinetics of excitatory synaptic transmission.
2010,
Pubmed Cau,
Regulation of Xenopus p21-activated kinase (X-PAK2) by Cdc42 and maturation-promoting factor controls Xenopus oocyte maturation.
2000,
Pubmed
,
Xenbase Cohen,
Protein phosphatase 4--from obscurity to vital functions.
2005,
Pubmed Fan,
Involvement of mitogen-activated protein kinase cascade during oocyte maturation and fertilization in mammals.
2004,
Pubmed Ferguson,
Ser-64 and Ser-111 in PHAS-I are dispensable for insulin-stimulated dissociation from eIF4E.
2003,
Pubmed Gietzen,
Identification of inhibitory autophosphorylation sites in casein kinase I epsilon.
1999,
Pubmed Gruhler,
Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway.
2005,
Pubmed Heesom,
Cell cycle-dependent phosphorylation of the translational repressor eIF-4E binding protein-1 (4E-BP1).
2001,
Pubmed Herbert,
The extracellular signal-regulated kinase pathway regulates the phosphorylation of 4E-BP1 at multiple sites.
2002,
Pubmed Hörmanseder,
Modulation of cell cycle control during oocyte-to-embryo transitions.
2013,
Pubmed
,
Xenbase Hornbeck,
PhosphoSitePlus: a comprehensive resource for investigating the structure and function of experimentally determined post-translational modifications in man and mouse.
2012,
Pubmed Huang,
Bioinformatics enrichment tools: paths toward the comprehensive functional analysis of large gene lists.
2009,
Pubmed Iwase,
A fully automated phosphopeptide purification system for large-scale phosphoproteome analysis.
2010,
Pubmed
,
Xenbase Kanemitsu,
Cdc2-mediated phosphorylation of the gap junction protein, connexin43, during mitosis.
1998,
Pubmed Kim,
Pathological roles of MAPK signaling pathways in human diseases.
2010,
Pubmed Krauchunas,
Molecular changes during egg activation.
2013,
Pubmed Kumano,
Calcium-mediated inactivation of the MAP kinase pathway in sea urchin eggs at fertilization.
2001,
Pubmed Kyono,
Successive and selective release of phosphorylated peptides captured by hydroxy acid-modified metal oxide chromatography.
2008,
Pubmed Lampe,
Formation of a distinct connexin43 phosphoisoform in mitotic cells is dependent upon p34cdc2 kinase.
1998,
Pubmed Liu,
Control of beta-catenin phosphorylation/degradation by a dual-kinase mechanism.
2002,
Pubmed
,
Xenbase Maloverjan,
Dual function of UNC-51-like kinase 3 (Ulk3) in the Sonic hedgehog signaling pathway.
2010,
Pubmed Maloverjan,
Identification of a novel serine/threonine kinase ULK3 as a positive regulator of Hedgehog pathway.
2010,
Pubmed Maudsley,
Gonadotropin-releasing hormone (GnRH) antagonists promote proapoptotic signaling in peripheral reproductive tumor cells by activating a Galphai-coupling state of the type I GnRH receptor.
2004,
Pubmed McGivern,
Toward defining the phosphoproteome of Xenopus laevis embryos.
2009,
Pubmed
,
Xenbase Mothe-Satney,
Mammalian target of rapamycin-dependent phosphorylation of PHAS-I in four (S/T)P sites detected by phospho-specific antibodies.
2000,
Pubmed Nakamura,
Ethylenediaminetetraacetic acid increases identification rate of phosphoproteomics in real biological samples.
2010,
Pubmed Park,
Discontinuous pH gradient-mediated separation of TiO2-enriched phosphopeptides.
2011,
Pubmed Pinkse,
Highly robust, automated, and sensitive online TiO2-based phosphoproteomics applied to study endogenous phosphorylation in Drosophila melanogaster.
2008,
Pubmed Radford,
Translational control by cytoplasmic polyadenylation in Xenopus oocytes.
2008,
Pubmed
,
Xenbase Sakanaka,
Casein kinase iepsilon in the wnt pathway: regulation of beta-catenin function.
1999,
Pubmed
,
Xenbase Simpson,
Trafficking, development and hedgehog.
2009,
Pubmed Sugiyama,
Phosphopeptide enrichment by aliphatic hydroxy acid-modified metal oxide chromatography for nano-LC-MS/MS in proteomics applications.
2007,
Pubmed Takano,
The binding of lamin B receptor to chromatin is regulated by phosphorylation in the RS region.
2002,
Pubmed
,
Xenbase Tape,
Reproducible automated phosphopeptide enrichment using magnetic TiO2 and Ti-IMAC.
2014,
Pubmed Thingholm,
Analytical strategies for phosphoproteomics.
2009,
Pubmed Tsai,
Sequential phosphoproteomic enrichment through complementary metal-directed immobilized metal ion affinity chromatography.
2014,
Pubmed Tunquist,
Under arrest: cytostatic factor (CSF)-mediated metaphase arrest in vertebrate eggs.
2003,
Pubmed
,
Xenbase Warn-Cramer,
Regulation of connexin-43 gap junctional intercellular communication by mitogen-activated protein kinase.
1998,
Pubmed Warn-Cramer,
Characterization of the mitogen-activated protein kinase phosphorylation sites on the connexin-43 gap junction protein.
1996,
Pubmed
