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Monoclonal antibodies identify a group of nuclear pore complex glycoproteins.
Snow CM, Senior A, Gerace L.
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Using monoclonal antibodies we identified a group of eight polypeptides of rat liver nuclear envelopes that have common epitopes. Most or all of these proteins are structurally distinct, as shown by tryptic peptide mapping and analysis with polyclonal antibodies. While these polypeptides are relatively tightly bound to nuclear membranes, only one is an integral membrane protein. The eight antigens cofractionate with the nuclear pore complex under various conditions of ionic strength and detergent. It can be seen by immunofluorescence microscopy that the monoclonal antibodies reacting with these antigens stain the nuclear surface of interphase cells in a finely punctate pattern. When the nuclear envelope is disassembled and subsequently reformed during mitosis, the proteins are reversibly dispersed throughout the cytoplasm in the form of minute foci. By EM immunogold localization on isolated nuclear envelopes, the monoclonal antibodies label exclusively the nuclear pore complex, at both its nucleoplasmic and cytoplasmic margins. Considered together, our biochemical and localization data indicate that the eight nuclear envelope polypeptides are pore complex components. As shown in the accompanying paper (Holt, G. D., C. M. Snow, A. Senior, R. S. Haltiwanger, L. Gerace, and G. W. Hart, J. Cell Biol., 104:1157-1164) these eight polypeptides contain a novel form of glycosylation, O-linked N-acetylglucosamine. The relative abundance and disposition of these O-linked glycoproteins in the pore complex are consistent with their having a role in nucleocytoplasmic transport.
Aaronson,
Isolation of nuclear pore complexes in association with a lamina.
1975, Pubmed
Aaronson,
Isolation of nuclear pore complexes in association with a lamina.
1975,
Pubmed AFZELIUS,
The ultrastructure of the nuclear membrane of the sea urchin oocyte as studied with the electron microscope.
1955,
Pubmed Berrios,
A 174-kilodalton ATPase/dATPase polypeptide and a glycoprotein of apparently identical molecular weight are common but distinct components of higher eukaryotic nuclear structural protein subfractions.
1983,
Pubmed Burke,
A monoclonal antibody against a 135-K Golgi membrane protein.
1982,
Pubmed Davis,
Identification and characterization of a nuclear pore complex protein.
1986,
Pubmed Dingwall,
A polypeptide domain that specifies migration of nucleoplasmin into the nucleus.
1982,
Pubmed
,
Xenbase Dwyer,
A modified procedure for the isolation of a pore complex-lamina fraction from rat liver nuclei.
1976,
Pubmed Elder,
Radioiodination of proteins in single polyacrylamide gel slices. Tryptic peptide analysis of all the major members of complex multicomponent systems using microgram quantities of total protein.
1977,
Pubmed Feldherr,
Evidence for mediated protein uptake by amphibian oocyte nuclei.
1983,
Pubmed Feldherr,
Movement of a karyophilic protein through the nuclear pores of oocytes.
1984,
Pubmed
,
Xenbase Fenner,
Quantification of Coomassie Blue stained proteins in polyacrylamide gels based on analyses of eluted dye.
1975,
Pubmed Franke,
The nuclear envelope and the architecture of the nuclear periphery.
1981,
Pubmed Gall,
Octagonal nuclear pores.
1967,
Pubmed Gerace,
Organization and modulation of nuclear lamina structure.
1984,
Pubmed Gerace,
Immunocytochemical localization of the major polypeptides of the nuclear pore complex-lamina fraction. Interphase and mitotic distribution.
1978,
Pubmed Gerace,
The nuclear envelope lamina is reversibly depolymerized during mitosis.
1980,
Pubmed Gerace,
Identification of a major polypeptide of the nuclear pore complex.
1982,
Pubmed Goldfarb,
Synthetic peptides as nuclear localization signals.
,
Pubmed
,
Xenbase GREENWOOD,
THE PREPARATION OF I-131-LABELLED HUMAN GROWTH HORMONE OF HIGH SPECIFIC RADIOACTIVITY.
1963,
Pubmed Hall,
Targeting of E. coli beta-galactosidase to the nucleus in yeast.
1984,
Pubmed Holt,
Nuclear pore complex glycoproteins contain cytoplasmically disposed O-linked N-acetylglucosamine.
1987,
Pubmed Holt,
The subcellular distribution of terminal N-acetylglucosamine moieties. Localization of a novel protein-saccharide linkage, O-linked GlcNAc.
1986,
Pubmed Hubbard,
Identification of rat hepatocyte plasma membrane proteins using monoclonal antibodies.
1985,
Pubmed Hubbard,
Isolation of rat hepatocyte plasma membranes. II. Identification of membrane-associated cytoskeletal proteins.
1983,
Pubmed Kalderon,
A short amino acid sequence able to specify nuclear location.
1984,
Pubmed Kay,
A method for the rapid isolation of nuclear membranes from rat liver. Characterisation of the membrane preparation and its associated DNA polymerase.
1972,
Pubmed Kearney,
A new mouse myeloma cell line that has lost immunoglobulin expression but permits the construction of antibody-secreting hybrid cell lines.
1979,
Pubmed Kiehart,
Monoclonal antibodies demonstrate limited structural homology between myosin isozymes from Acanthamoeba.
1984,
Pubmed Kornfeld,
Assembly of asparagine-linked oligosaccharides.
1985,
Pubmed Krohne,
Localization of a nuclear envelope-associated protein by indirect immunofluorescence microscopy using antibodies against a major polypeptide from rat liver fractions enriched in nuclear envelope-associated material.
1978,
Pubmed Krohne,
The nuclear lamins. A multigene family of proteins in evolution and differentiation.
1986,
Pubmed
,
Xenbase Lanford,
Induction of nuclear transport with a synthetic peptide homologous to the SV40 T antigen transport signal.
1986,
Pubmed Lang,
Molecular mobility and nucleocytoplasmic flux in hepatoma cells.
1986,
Pubmed LOWRY,
Protein measurement with the Folin phenol reagent.
1951,
Pubmed Maul,
The nuclear and the cytoplasmic pore complex: structure, dynamics, distribution, and evolution.
1977,
Pubmed Ottaviano,
Phosphorylation of the nuclear lamins during interphase and mitosis.
1985,
Pubmed Paine,
Nuclear envelope permeability.
1975,
Pubmed Steck,
Selective solubilization of proteins from red blood cell membranes by protein perturbants.
1973,
Pubmed Torres,
Topography and polypeptide distribution of terminal N-acetylglucosamine residues on the surfaces of intact lymphocytes. Evidence for O-linked GlcNAc.
1984,
Pubmed Unwin,
A large particle associated with the perimeter of the nuclear pore complex.
1982,
Pubmed
,
Xenbase Warren,
Recycling of transferrin receptors in A431 cells is inhibited during mitosis.
1984,
Pubmed
