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In situ hybridization is the technique by which specific RNA or DNA molecules are detected in cytological preparations. Basically it involves formation of a hybrid molecule between an endogenous single-stranded RNA or DNA in the cell and a complementary single-stranded RNA or DNA probe. In its original form the probe was labeled with (3)H and the hybrid was detected by autoradiography. The first successful experiments in 1968 involved detection of the highly amplified ribosomal DNA in oocytes of the frog Xenopus, followed soon after by the reiterated "satellite DNA" in mouse and Drosophila chromosomes. Fluorescent probes were developed about ten years later.
Bauman,
A new method for fluorescence microscopical localization of specific DNA sequences by in situ hybridization of fluorochromelabelled RNA.
1980, Pubmed
Bauman,
A new method for fluorescence microscopical localization of specific DNA sequences by in situ hybridization of fluorochromelabelled RNA.
1980,
Pubmed Bauman,
Rapid and high resolution detection of in situ hybridisation to polytene chromosomes using fluorochrome-labeled RNA.
1981,
Pubmed Brown,
Specific gene amplification in oocytes. Oocyte nuclei contain extrachromosomal replicas of the genes for ribosomal RNA.
1968,
Pubmed BROWN,
ABSENCE OF RIBOSOMAL RNA SYNTHESIS IN THE ANUCLEOLATE MUTANT OF XENOPUS LAEVIS.
1964,
Pubmed
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Xenbase Buongiorno-Nardelli,
Autoradiographic detection of molecular hybrids between RNA and DNA in tissue sections.
1970,
Pubmed COONS,
Localization of antigen in tissue cells; improvements in a method for the detection of antigen by means of fluorescent antibody.
1950,
Pubmed Gall,
Early studies on gene amplification.
1978,
Pubmed Gall,
Formation and detection of RNA-DNA hybrid molecules in cytological preparations.
1969,
Pubmed
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Xenbase Gall,
Reptitive DNA sequences in drosophila.
1971,
Pubmed Gall,
The genes for ribosomal RNA during oögenesis.
1969,
Pubmed Gall,
Gene amplification in the oocytes of Dytiscid water beetles.
1969,
Pubmed Gall,
Differential synthesis of the genes for ribosomal RNA during amphibian oögenesis.
1968,
Pubmed Gillespie,
A quantitative assay for DNA-RNA hybrids with DNA immobilized on a membrane.
1965,
Pubmed John,
RNA-DNA hybrids at the cytological level.
1969,
Pubmed KIT,
Equilibrium sedimentation in density gradients of DNA preparations from animal tissues.
1961,
Pubmed Langer-Safer,
Immunological method for mapping genes on Drosophila polytene chromosomes.
1982,
Pubmed Meselson,
THE REPLICATION OF DNA IN ESCHERICHIA COLI.
1958,
Pubmed Miller,
Structure and composition of peripheral nucleoli of salamander oocytes.
1966,
Pubmed Painter,
Nucleic Acid Storage in the Toad's Egg.
1942,
Pubmed Pardu,
Cytological localization of DNA complementary to ribosomal RNA in polytene chromosomes of Diptera.
1970,
Pubmed Pardue,
Chromosomal localization of mouse satellite DNA.
1970,
Pubmed Peacock,
Chromosome replication.
1965,
Pubmed RITOSSA,
LOCALIZATION OF DNA COMPLEMENTARY TO RIBOSOMAL RNA IN THE NUCLEOLUS ORGANIZER REGION OF DROSOPHILA MELANOGASTER.
1965,
Pubmed Rudkin,
High resolution detection of DNA-RNA hybrids in situ by indirect immunofluorescence.
1977,
Pubmed Wallace,
Ribosomal cistrons and the nucleolar organizer.
1966,
Pubmed Waring,
Nucleotide sequence repetition: a rapidly reassociating fraction of mouse DNA.
1966,
Pubmed
