Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Dev Biol
2016 Apr 01;4121:18-31. doi: 10.1016/j.ydbio.2016.02.017.
Show Gene links
Show Anatomy links
Spatial regulation of cell cohesion by Wnt5a during second heart field progenitor deployment.
Li D, Sinha T, Ajima R, Seo HS, Yamaguchi TP, Wang J.
???displayArticle.abstract??? Wnt5a, a non-canonical Wnt ligand critical for outflow tract (OFT) morphogenesis, is expressed specifically in second heart field (SHF) progenitors in the caudal splanchnic mesoderm (SpM) near the inflow tract (IFT). Using a conditional Wnt5a gain of function (GOF) allele and Islet1-Cre, we broadly over-expressed Wnt5a throughout the SHF lineage, including the entire SpM between the IFT and OFT. Wnt5a over-expression in Wnt5a null mutants can rescue the cell polarity and actin polymerization defects as well as severe SpM shortening, but fails to rescue OFT shortening. Moreover, Wnt5a over-expression in wild-type background is able to cause OFT shortening. We find that Wnt5a over-expression does not perturb SHF cell proliferation, apoptosis or differentiation, but affects the deployment of SHF cells by causing them to accumulate into a large bulge at the rostral SpM and fail to enter the OFT. Our immunostaining analyses suggest an inverse correlation between cell cohesion and Wnt5a level in the wild-type SpM. Ectopic Wnt5a expression in the rostral SpM of Wn5a-GOF mutants diminishes the upregulation of adherens junction; whereas loss of Wnt5a in Wnt5a null mutants causes premature increase in adherens junction level in the caudal SpM. Over-expression of mouse Wnt5a in Xenopus animal cap cells also reduces C-cadherin distribution on the plasma membrane without affecting its overall protein level, suggesting that Wnt5a may play an evolutionarily conserved role in controlling the cell surface level of cadherin to modulate cell cohesion during tissue morphogenesis. Collectively, our data indicate that restricted expression of Wnt5a in the caudal SpM is essential for normal OFT morphogenesis, and uncover a novel function of spatially regulated cell cohesion by Wnt5a in driving the deployment of SHF cells from the SpM into the OFT.
Ai,
Canonical Wnt signaling functions in second heart field to promote right ventricular growth.
2007, Pubmed
Ai,
Canonical Wnt signaling functions in second heart field to promote right ventricular growth.
2007,
Pubmed Black,
Transcriptional pathways in second heart field development.
2007,
Pubmed Cai,
Isl1 identifies a cardiac progenitor population that proliferates prior to differentiation and contributes a majority of cells to the heart.
2003,
Pubmed Cha,
Appropriate crypt formation in the uterus for embryo homing and implantation requires Wnt5a-ROR signaling.
2014,
Pubmed Choi,
Xenopus Cdc42 regulates convergent extension movements during gastrulation through Wnt/Ca2+ signaling pathway.
2002,
Pubmed
,
Xenbase Cohen,
Wnt5a and Wnt11 are essential for second heart field progenitor development.
2012,
Pubmed Cohen,
Wnt/beta-catenin signaling promotes expansion of Isl-1-positive cardiac progenitor cells through regulation of FGF signaling.
2007,
Pubmed Devenport,
The cell biology of planar cell polarity.
2014,
Pubmed Dyer,
The role of secondary heart field in cardiac development.
2009,
Pubmed Evans,
Myocardial lineage development.
2010,
Pubmed Francou,
TBX1 regulates epithelial polarity and dynamic basal filopodia in the second heart field.
2014,
Pubmed Goodrich,
Principles of planar polarity in animal development.
2011,
Pubmed Gray,
Planar cell polarity: coordinating morphogenetic cell behaviors with embryonic polarity.
2011,
Pubmed Habas,
Wnt/Frizzled activation of Rho regulates vertebrate gastrulation and requires a novel Formin homology protein Daam1.
2001,
Pubmed
,
Xenbase Jho,
Wnt/beta-catenin/Tcf signaling induces the transcription of Axin2, a negative regulator of the signaling pathway.
2002,
Pubmed Jiao,
Tgfbeta signaling is required for atrioventricular cushion mesenchyme remodeling during in vivo cardiac development.
2006,
Pubmed Kelly,
The arterial pole of the mouse heart forms from Fgf10-expressing cells in pharyngeal mesoderm.
2001,
Pubmed Kühl,
The Wnt/Ca2+ pathway: a new vertebrate Wnt signaling pathway takes shape.
2000,
Pubmed Kwon,
A regulatory pathway involving Notch1/beta-catenin/Isl1 determines cardiac progenitor cell fate.
2009,
Pubmed Lin,
Beta-catenin directly regulates Islet1 expression in cardiovascular progenitors and is required for multiple aspects of cardiogenesis.
2007,
Pubmed Linask,
N-cadherin-catenin interaction: necessary component of cardiac cell compartmentalization during early vertebrate heart development.
1997,
Pubmed Linask,
N-cadherin localization in early heart development and polar expression of Na+,K(+)-ATPase, and integrin during pericardial coelom formation and epithelialization of the differentiating myocardium.
1992,
Pubmed Mikels,
Purified Wnt5a protein activates or inhibits beta-catenin-TCF signaling depending on receptor context.
2006,
Pubmed Mjaatvedt,
The outflow tract of the heart is recruited from a novel heart-forming field.
2001,
Pubmed Ninomiya,
Antero-posterior tissue polarity links mesoderm convergent extension to axial patterning.
2004,
Pubmed
,
Xenbase Radice,
Developmental defects in mouse embryos lacking N-cadherin.
1997,
Pubmed Ramsbottom,
Vangl2-regulated polarisation of second heart field-derived cells is required for outflow tract lengthening during cardiac development.
2014,
Pubmed Schleiffarth,
Wnt5a is required for cardiac outflow tract septation in mice.
2007,
Pubmed Sinha,
Disheveled mediated planar cell polarity signaling is required in the second heart field lineage for outflow tract morphogenesis.
2012,
Pubmed Sinha,
Loss of Wnt5a disrupts second heart field cell deployment and may contribute to OFT malformations in DiGeorge syndrome.
2015,
Pubmed Sinha,
Mapping the dynamic expression of Wnt11 and the lineage contribution of Wnt11-expressing cells during early mouse development.
2015,
Pubmed
,
Xenbase Soh,
N-cadherin prevents the premature differentiation of anterior heart field progenitors in the pharyngeal mesodermal microenvironment.
2014,
Pubmed Tada,
Convergent extension: using collective cell migration and cell intercalation to shape embryos.
2012,
Pubmed
,
Xenbase Topol,
Wnt-5a inhibits the canonical Wnt pathway by promoting GSK-3-independent beta-catenin degradation.
2003,
Pubmed Torres,
Activities of the Wnt-1 class of secreted signaling factors are antagonized by the Wnt-5A class and by a dominant negative cadherin in early Xenopus development.
1996,
Pubmed
,
Xenbase Ulrich,
Wnt11 functions in gastrulation by controlling cell cohesion through Rab5c and E-cadherin.
2005,
Pubmed van den Berg,
A caudal proliferating growth center contributes to both poles of the forming heart tube.
2009,
Pubmed Vincent,
How to make a heart: the origin and regulation of cardiac progenitor cells.
2010,
Pubmed Waldo,
Conotruncal myocardium arises from a secondary heart field.
2001,
Pubmed
,
Xenbase Wang,
Wnt signaling in mammalian development: lessons from mouse genetics.
2012,
Pubmed Wang,
Disruption of PCP signaling causes limb morphogenesis and skeletal defects and may underlie Robinow syndrome and brachydactyly type B.
2011,
Pubmed Xin,
Mending broken hearts: cardiac development as a basis for adult heart regeneration and repair.
2013,
Pubmed Yamaguchi,
A Wnt5a pathway underlies outgrowth of multiple structures in the vertebrate embryo.
1999,
Pubmed Yang,
Isl1Cre reveals a common Bmp pathway in heart and limb development.
2006,
Pubmed Zallen,
Planar polarity and tissue morphogenesis.
2007,
Pubmed Zhou,
Modulation of morphogenesis by noncanonical Wnt signaling requires ATF/CREB family-mediated transcriptional activation of TGFbeta2.
2007,
Pubmed
