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Feric M, Vaidya N, Harmon TS, Mitrea DM, Zhu L, Richardson TM, Kriwacki RW, Pappu RV, Brangwynne CP.
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The nucleolus and other ribonucleoprotein (RNP) bodies are membrane-less organelles that appear to assemble through phase separation of their molecular components. However, many such RNP bodies contain internal subcompartments, and the mechanism of their formation remains unclear. Here, we combine in vivo and in vitro studies, together with computational modeling, to show that subcompartments within the nucleolus represent distinct, coexisting liquid phases. Consistent with their in vivo immiscibility, purified nucleolar proteins phase separate into droplets containing distinct non-coalescing phases that are remarkably similar to nucleoli in vivo. This layered droplet organization is caused by differences in the biophysical properties of the phases-particularly droplet surface tension-which arises from sequence-encoded features of their macromolecular components. These results suggest that phase separation can give rise to multilayered liquids that may facilitate sequential RNA processing reactions in a variety of RNP bodies. PAPERCLIP.
Aarts,
Direct visual observation of thermal capillary waves.
2004, Pubmed
Aarts,
Direct visual observation of thermal capillary waves.
2004,
Pubmed Balagopal,
Polysomes, P bodies and stress granules: states and fates of eukaryotic mRNAs.
2009,
Pubmed Berry,
RNA transcription modulates phase transition-driven nuclear body assembly.
2015,
Pubmed Boisvert,
The multifunctional nucleolus.
2007,
Pubmed Brangwynne,
Germline P granules are liquid droplets that localize by controlled dissolution/condensation.
2009,
Pubmed Brangwynne,
Active liquid-like behavior of nucleoli determines their size and shape in Xenopus laevis oocytes.
2011,
Pubmed
,
Xenbase Buchan,
Eukaryotic stress granules: the ins and outs of translation.
2009,
Pubmed Derenzini,
Nucleolar size indicates the rapidity of cell proliferation in cancer tissues.
2000,
Pubmed Elbaum-Garfinkle,
The disordered P granule protein LAF-1 drives phase separation into droplets with tunable viscosity and dynamics.
2015,
Pubmed Feric,
A nuclear F-actin scaffold stabilizes ribonucleoprotein droplets against gravity in large cells.
2013,
Pubmed
,
Xenbase Feric,
Soft viscoelastic properties of nuclear actin age oocytes due to gravitational creep.
2015,
Pubmed
,
Xenbase Foty,
Surface tensions of embryonic tissues predict their mutual envelopment behavior.
1996,
Pubmed Frank,
ncl-1 is required for the regulation of cell size and ribosomal RNA synthesis in Caenorhabditis elegans.
1998,
Pubmed Gall,
Cajal bodies: the first 100 years.
2000,
Pubmed Gigova,
A cluster of methylations in the domain IV of 25S rRNA is required for ribosome stability.
2014,
Pubmed Hennig,
Prion-like domains in RNA binding proteins are essential for building subnuclear paraspeckles.
2015,
Pubmed Henras,
The post-transcriptional steps of eukaryotic ribosome biogenesis.
2008,
Pubmed Hubstenberger,
Translation repressors, an RNA helicase, and developmental cues control RNP phase transitions during early development.
2013,
Pubmed Jain,
ATPase-Modulated Stress Granules Contain a Diverse Proteome and Substructure.
2016,
Pubmed Kedersha,
Stress granules and processing bodies are dynamically linked sites of mRNP remodeling.
2005,
Pubmed Kroschwald,
Promiscuous interactions and protein disaggregases determine the material state of stress-inducible RNP granules.
2015,
Pubmed Krüger,
Intranucleolar sites of ribosome biogenesis defined by the localization of early binding ribosomal proteins.
2007,
Pubmed Li,
Phase transitions in the assembly of multivalent signalling proteins.
2012,
Pubmed Lin,
Formation and Maturation of Phase-Separated Liquid Droplets by RNA-Binding Proteins.
2015,
Pubmed Mitrea,
Structural polymorphism in the N-terminal oligomerization domain of NPM1.
2014,
Pubmed Mitrea,
Nucleophosmin integrates within the nucleolus via multi-modal interactions with proteins displaying R-rich linear motifs and rRNA.
2016,
Pubmed Molliex,
Phase separation by low complexity domains promotes stress granule assembly and drives pathological fibrillization.
2015,
Pubmed Nott,
Phase transition of a disordered nuage protein generates environmentally responsive membraneless organelles.
2015,
Pubmed Ochs,
Fibrillarin: a new protein of the nucleolus identified by autoimmune sera.
1985,
Pubmed
,
Xenbase Parry,
The bacterial cytoplasm has glass-like properties and is fluidized by metabolic activity.
2014,
Pubmed Patel,
A Liquid-to-Solid Phase Transition of the ALS Protein FUS Accelerated by Disease Mutation.
2015,
Pubmed Pederson,
The nucleolus.
2011,
Pubmed Shav-Tal,
Dynamic sorting of nuclear components into distinct nucleolar caps during transcriptional inhibition.
2005,
Pubmed Spector,
Nuclear domains.
2001,
Pubmed Thiry,
Birth of a nucleolus: the evolution of nucleolar compartments.
2005,
Pubmed Tschochner,
Pre-ribosomes on the road from the nucleolus to the cytoplasm.
2003,
Pubmed Wang,
Regulation of RNA granule dynamics by phosphorylation of serine-rich, intrinsically disordered proteins in C. elegans.
2014,
Pubmed Weber,
Getting RNA and protein in phase.
2012,
Pubmed Weber,
Inverse size scaling of the nucleolus by a concentration-dependent phase transition.
2015,
Pubmed Wippich,
Dual specificity kinase DYRK3 couples stress granule condensation/dissolution to mTORC1 signaling.
2013,
Pubmed Xiang,
The LC Domain of hnRNPA2 Adopts Similar Conformations in Hydrogel Polymers, Liquid-like Droplets, and Nuclei.
2015,
Pubmed Zhang,
RNA Controls PolyQ Protein Phase Transitions.
2015,
Pubmed
