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The model system, Xenopus laevis, has been used in innumerable research studies and has contributed to the understanding of multiple cytoskeletal components, including actin, microtubules, and neurofilaments, during axon pathfinding. Xenopus developmental stages have been widely characterized, and the Xenopus genome has been sequenced, allowing gene expression modifications through exogenous molecules. Xenopus cell cultures are ideal for long periods of live imaging because they are easily obtained and maintained, and they do not require special culture conditions. In addition, Xenopus have relatively large growth cones, compared to other vertebrates, thus providing a suitable system for imaging cytoskeletal components. Therefore, X. laevis is an ideal model organism in which to study cytoskeletal dynamics during axon pathfinding.
Andersen,
Axon formation: a molecular model for the generation of neuronal polarity.
2000, Pubmed
Andersen,
Axon formation: a molecular model for the generation of neuronal polarity.
2000,
Pubmed Applegate,
plusTipTracker: Quantitative image analysis software for the measurement of microtubule dynamics.
2011,
Pubmed Bearce,
TIPsy tour guides: how microtubule plus-end tracking proteins (+TIPs) facilitate axon guidance.
2015,
Pubmed Bestman,
In vivo single-cell electroporation for transfer of DNA and macromolecules.
2006,
Pubmed
,
Xenbase Blum,
Morpholinos: Antisense and Sensibility.
2015,
Pubmed
,
Xenbase Bowes,
Xenbase: a Xenopus biology and genomics resource.
2008,
Pubmed
,
Xenbase Burkel,
Versatile fluorescent probes for actin filaments based on the actin-binding domain of utrophin.
2007,
Pubmed
,
Xenbase Cammarata,
Cytoskeletal social networking in the growth cone: How +TIPs mediate microtubule-actin cross-linking to drive axon outgrowth and guidance.
2016,
Pubmed Chapman,
Targeted Germline Modifications in Rats Using CRISPR/Cas9 and Spermatogonial Stem Cells.
2015,
Pubmed Chien,
Axonal guidance from retina to tectum in embryonic Xenopus.
1994,
Pubmed
,
Xenbase Dent,
The growth cone cytoskeleton in axon outgrowth and guidance.
2011,
Pubmed Dickson,
Molecular mechanisms of axon guidance.
2002,
Pubmed Dontchev,
Growth cones integrate signaling from multiple guidance cues.
2003,
Pubmed Eide,
Electroporation-mediated gene transfer in free-swimming embryonic Xenopus laevis.
2000,
Pubmed
,
Xenbase Eisen,
Controlling morpholino experiments: don't stop making antisense.
2008,
Pubmed
,
Xenbase Engel,
Structured illumination superresolution imaging of the cytoskeleton.
2014,
Pubmed Erdogan,
Using Xenopus laevis retinal and spinal neurons to study mechanisms of axon guidance in vivo and in vitro.
2016,
Pubmed
,
Xenbase Fantetti,
Dissection and culture of chick statoacoustic ganglion and spinal cord explants in collagen gels for neurite outgrowth assays.
2011,
Pubmed Gomez,
Actin dynamics in growth cone motility and navigation.
2014,
Pubmed Gómez,
Working with Xenopus spinal neurons in live cell culture.
2003,
Pubmed
,
Xenbase Guo,
Efficient RNA/Cas9-mediated genome editing in Xenopus tropicalis.
2014,
Pubmed
,
Xenbase Hellsten,
The genome of the Western clawed frog Xenopus tropicalis.
2010,
Pubmed
,
Xenbase Holy,
Dynamic instability of microtubules as an efficient way to search in space.
1994,
Pubmed Honnappa,
An EB1-binding motif acts as a microtubule tip localization signal.
2009,
Pubmed Kaang,
Parameters influencing ectopic gene expression in Aplysia neurons.
1996,
Pubmed Kaech,
Culturing hippocampal neurons.
2006,
Pubmed Keller,
Early embryonic development of Xenopus laevis.
1991,
Pubmed
,
Xenbase Klein,
Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
2002,
Pubmed
,
Xenbase Lee,
Neuronal cell cultures from aplysia for high-resolution imaging of growth cones.
2008,
Pubmed Lin,
Myosin drives retrograde F-actin flow in neuronal growth cones.
1996,
Pubmed Lin,
Neurofilaments help maintain normal morphologies and support elongation of neurites in Xenopus laevis cultured embryonic spinal cord neurons.
1995,
Pubmed
,
Xenbase Lin,
Growth cone advance is inversely proportional to retrograde F-actin flow.
1995,
Pubmed Lowery,
The trip of the tip: understanding the growth cone machinery.
2009,
Pubmed
,
Xenbase Lowery,
Growth cone-specific functions of XMAP215 in restricting microtubule dynamics and promoting axonal outgrowth.
2013,
Pubmed
,
Xenbase Lowery,
Neural Explant Cultures from Xenopus laevis.
2012,
Pubmed
,
Xenbase Lucaj,
Xenopus TACC1 is a microtubule plus-end tracking protein that can regulate microtubule dynamics during embryonic development.
2015,
Pubmed
,
Xenbase Mallavarapu,
Regulated actin cytoskeleton assembly at filopodium tips controls their extension and retraction.
1999,
Pubmed Marx,
Xenopus cytoplasmic linker-associated protein 1 (XCLASP1) promotes axon elongation and advance of pioneer microtubules.
2013,
Pubmed
,
Xenbase Mendoza,
Quantitative fluorescent speckle microscopy (QFSM) to measure actin dynamics.
2012,
Pubmed Momose,
Efficient targeting of gene expression in chick embryos by microelectroporation.
1999,
Pubmed Moody,
Fates of the blastomeres of the 16-cell stage Xenopus embryo.
1987,
Pubmed
,
Xenbase Moody,
Fates of the blastomeres of the 32-cell-stage Xenopus embryo.
1987,
Pubmed
,
Xenbase Moon,
Balanced Vav2 GEF activity regulates neurite outgrowth and branching in vitro and in vivo.
2010,
Pubmed
,
Xenbase Myers,
Focal adhesion kinase modulates Cdc42 activity downstream of positive and negative axon guidance cues.
2012,
Pubmed
,
Xenbase Nakajima,
Highly efficient gene knockout by injection of TALEN mRNAs into oocytes and host transfer in Xenopus laevis.
2015,
Pubmed
,
Xenbase Nichol,
Guidance of Axons by Local Coupling of Retrograde Flow to Point Contact Adhesions.
2016,
Pubmed
,
Xenbase Nwagbara,
TACC3 is a microtubule plus end-tracking protein that promotes axon elongation and also regulates microtubule plus end dynamics in multiple embryonic cell types.
2014,
Pubmed
,
Xenbase Okabe,
Actin dynamics in growth cones.
1991,
Pubmed Paglini,
Suppression of radixin and moesin alters growth cone morphology, motility, and process formation in primary cultured neurons.
1998,
Pubmed Petchprayoon,
Fluorescent kabiramides: new probes to quantify actin in vitro and in vivo.
2005,
Pubmed Ren,
Increase in Growth Cone Size Correlates with Decrease in Neurite Growth Rate.
2016,
Pubmed Rutherford,
Xenopus TACC2 is a microtubule plus end-tracking protein that can promote microtubule polymerization during embryonic development.
2016,
Pubmed
,
Xenbase Sahly,
Effective expression of the green fluorescent fusion proteins in cultured Aplysia neurons.
2003,
Pubmed Sakane,
Targeted mutagenesis of multiple and paralogous genes in Xenopus laevis using two pairs of transcription activator-like effector nucleases.
2014,
Pubmed
,
Xenbase Santiago-Medina,
Imaging adhesion and signaling dynamics in Xenopus laevis growth cones.
2012,
Pubmed
,
Xenbase Schnell,
Immunolabeling artifacts and the need for live-cell imaging.
2012,
Pubmed Session,
Genome evolution in the allotetraploid frog Xenopus laevis.
2016,
Pubmed
,
Xenbase Smith,
Neurofilament content is correlated with branch length in developing collateral branches of Xenopus spinal cord neurons.
2006,
Pubmed
,
Xenbase Stepanova,
Visualization of microtubule growth in cultured neurons via the use of EB3-GFP (end-binding protein 3-green fluorescent protein).
2003,
Pubmed Stout,
Using plusTipTracker software to measure microtubule dynamics in Xenopus laevis growth cones.
2014,
Pubmed
,
Xenbase Suter,
The emerging role of forces in axonal elongation.
2011,
Pubmed
,
Xenbase Suzuki,
High efficiency TALENs enable F0 functional analysis by targeted gene disruption in Xenopus laevis embryos.
2013,
Pubmed
,
Xenbase Tabata,
Efficient in utero gene transfer system to the developing mouse brain using electroporation: visualization of neuronal migration in the developing cortex.
2001,
Pubmed Tanaka,
The role of microtubule dynamics in growth cone motility and axonal growth.
1995,
Pubmed
,
Xenbase Tosney,
Growth cone morphology and trajectory in the lumbosacral region of the chick embryo.
1985,
Pubmed Undamatla,
Differential expression and localization of neuronal intermediate filament proteins within newly developing neurites in dissociated cultures of Xenopus laevis embryonic spinal cord.
2001,
Pubmed
,
Xenbase Woo,
Rac1 and RhoA promote neurite outgrowth through formation and stabilization of growth cone point contacts.
2006,
Pubmed
,
Xenbase Xu,
Dual-objective STORM reveals three-dimensional filament organization in the actin cytoskeleton.
2012,
Pubmed Zhou,
How actin filaments and microtubules steer growth cones to their targets.
2004,
Pubmed
