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???displayArticle.abstract??? Spindle assembly requires the coordinated action of multiple cellular structures to nucleate and organize microtubules in a precise spatiotemporal manner. Among them, the contributions of centrosomes, chromosomes, and microtubules have been well studied, yet the involvement of membrane-bound organelles remains largely elusive. Here, we provide mechanistic evidence for a membrane-based, Golgi-derived microtubule assembly pathway in mitosis. Upon mitotic entry, the Golgi matrix protein GM130 interacts with importin α via a classical nuclear localization signal that recruits importin α to the Golgi membranes. Sequestration of importin α by GM130 liberates the spindle assembly factor TPX2, which activates Aurora-A kinase and stimulates local microtubule nucleation. Upon filament assembly, nascent microtubules are further captured by GM130, thus linking Golgi membranes to the spindle. Our results reveal an active role for the Golgi in regulating spindle formation to ensure faithful organelle inheritance.
Baschieri,
Spatial control of Cdc42 signalling by a GM130-RasGRF complex regulates polarity and tumorigenesis.
2014, Pubmed
Baschieri,
Spatial control of Cdc42 signalling by a GM130-RasGRF complex regulates polarity and tumorigenesis.
2014,
Pubmed Basto,
Flies without centrioles.
2006,
Pubmed Bayliss,
Structural basis of Aurora-A activation by TPX2 at the mitotic spindle.
2003,
Pubmed
,
Xenbase Bazzi,
Acentriolar mitosis activates a p53-dependent apoptosis pathway in the mouse embryo.
2014,
Pubmed Carazo-Salas,
Generation of GTP-bound Ran by RCC1 is required for chromatin-induced mitotic spindle formation.
1999,
Pubmed
,
Xenbase Chabin-Brion,
The Golgi complex is a microtubule-organizing organelle.
2001,
Pubmed Chang,
Interaction between Poly(ADP-ribose) and NuMA contributes to mitotic spindle pole assembly.
2009,
Pubmed Cutress,
Structural basis for the nuclear import of the human androgen receptor.
2008,
Pubmed Diao,
The coiled-coil membrane protein golgin-84 is a novel rab effector required for Golgi ribbon formation.
2003,
Pubmed Dumont,
A centriole- and RanGTP-independent spindle assembly pathway in meiosis I of vertebrate oocytes.
2007,
Pubmed
,
Xenbase Efimov,
Asymmetric CLASP-dependent nucleation of noncentrosomal microtubules at the trans-Golgi network.
2007,
Pubmed Giesecke,
Novel binding of the mitotic regulator TPX2 (target protein for Xenopus kinesin-like protein 2) to importin-alpha.
2010,
Pubmed
,
Xenbase Goshima,
Augmin: a protein complex required for centrosome-independent microtubule generation within the spindle.
2008,
Pubmed Gruss,
Ran induces spindle assembly by reversing the inhibitory effect of importin alpha on TPX2 activity.
2001,
Pubmed
,
Xenbase Hachet,
Importin alpha associates with membranes and participates in nuclear envelope assembly in vitro.
2004,
Pubmed
,
Xenbase Hehnly,
Rab11 endosomes contribute to mitotic spindle organization and orientation.
2014,
Pubmed Heidebrecht,
p100: a novel proliferation-associated nuclear protein specifically restricted to cell cycle phases S, G2, and M.
1997,
Pubmed Ishida,
GM130 is a parallel tetramer with a flexible rod-like structure and N-terminally open (Y-shaped) and closed (I-shaped) conformations.
2015,
Pubmed Jesch,
The Golgi and endoplasmic reticulum remain independent during mitosis in HeLa cells.
1998,
Pubmed Jokitalo,
Golgi clusters and vesicles mediate mitotic inheritance independently of the endoplasmic reticulum.
2001,
Pubmed Kalab,
The ran GTPase regulates mitotic spindle assembly.
1999,
Pubmed
,
Xenbase Kalab,
The RanGTP gradient - a GPS for the mitotic spindle.
2008,
Pubmed Kaláb,
Analysis of a RanGTP-regulated gradient in mitotic somatic cells.
2006,
Pubmed
,
Xenbase Khodjakov,
Centrosome-independent mitotic spindle formation in vertebrates.
2000,
Pubmed Kodani,
The Golgi protein GM130 regulates centrosome morphology and function.
2008,
Pubmed Levine,
Binding of the vesicle docking protein p115 to Golgi membranes is inhibited under mitotic conditions.
1996,
Pubmed Lowe,
Cdc2 kinase directly phosphorylates the cis-Golgi matrix protein GM130 and is required for Golgi fragmentation in mitosis.
1998,
Pubmed Lowe,
The mitotic phosphorylation cycle of the cis-Golgi matrix protein GM130.
2000,
Pubmed Meunier,
Microtubule assembly during mitosis - from distinct origins to distinct functions?
2012,
Pubmed Nakamura,
The vesicle docking protein p115 binds GM130, a cis-Golgi matrix protein, in a mitotically regulated manner.
1997,
Pubmed Niclas,
Cell cycle regulation of dynein association with membranes modulates microtubule-based organelle transport.
1996,
Pubmed
,
Xenbase Oddoux,
Microtubules that form the stationary lattice of muscle fibers are dynamic and nucleated at Golgi elements.
2013,
Pubmed Ohba,
Self-organization of microtubule asters induced in Xenopus egg extracts by GTP-bound Ran.
1999,
Pubmed
,
Xenbase Ori-McKenney,
Golgi outposts shape dendrite morphology by functioning as sites of acentrosomal microtubule nucleation in neurons.
2012,
Pubmed Petry,
Branching microtubule nucleation in Xenopus egg extracts mediated by augmin and TPX2.
2013,
Pubmed
,
Xenbase Pollard,
A novel receptor-mediated nuclear protein import pathway.
1996,
Pubmed Preisinger,
YSK1 is activated by the Golgi matrix protein GM130 and plays a role in cell migration through its substrate 14-3-3zeta.
2004,
Pubmed Rivero,
Microtubule nucleation at the cis-side of the Golgi apparatus requires AKAP450 and GM130.
2009,
Pubmed Sardon,
Dissecting the role of Aurora A during spindle assembly.
2008,
Pubmed
,
Xenbase Sauer,
Proteome analysis of the human mitotic spindle.
2005,
Pubmed Schatz,
Importin alpha-regulated nucleation of microtubules by TPX2.
2003,
Pubmed
,
Xenbase Seemann,
The role of the tethering proteins p115 and GM130 in transport through the Golgi apparatus in vivo.
2000,
Pubmed Seemann,
Partitioning of the matrix fraction of the Golgi apparatus during mitosis in animal cells.
2002,
Pubmed Shima,
An ordered inheritance strategy for the Golgi apparatus: visualization of mitotic disassembly reveals a role for the mitotic spindle.
1998,
Pubmed Shima,
Partitioning of the Golgi apparatus during mitosis in living HeLa cells.
1997,
Pubmed Tang,
Reconstitution of the cell cycle-regulated Golgi disassembly and reassembly in a cell-free system.
2010,
Pubmed Taylor,
Characterization of the Golgi complex cleared of proteins in transit and examination of calcium uptake activities.
1997,
Pubmed Tsai,
A Ran signalling pathway mediated by the mitotic kinase Aurora A in spindle assembly.
2003,
Pubmed
,
Xenbase Wei,
The mitotic spindle mediates inheritance of the Golgi ribbon structure.
2009,
Pubmed Wei,
Mitotic division of the mammalian Golgi apparatus.
2009,
Pubmed Wiese,
Role of importin-beta in coupling Ran to downstream targets in microtubule assembly.
2001,
Pubmed
,
Xenbase Wilbur,
Mitotic spindle scaling during Xenopus development by kif2a and importin α.
2013,
Pubmed
,
Xenbase Wilde,
Stimulation of microtubule aster formation and spindle assembly by the small GTPase Ran.
1999,
Pubmed
,
Xenbase Zhang,
GM130, a cis-Golgi protein, regulates meiotic spindle assembly and asymmetric division in mouse oocyte.
2011,
Pubmed Zheng,
A membranous spindle matrix orchestrates cell division.
2010,
Pubmed
