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Genes Dev
2003 May 01;179:1141-52. doi: 10.1101/gad.1070003.
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A novel ring-like complex of Xenopus proteins essential for the initiation of DNA replication.
Kubota Y, Takase Y, Komori Y, Hashimoto Y, Arata T, Kamimura Y, Araki H, Takisawa H.
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We have identified Xenopus homologs of the budding yeast Sld5 and its three interacting proteins. These form a novel complex essential for the initiation of DNA replication in Xenopus egg extracts. The complex binds to chromatin in a manner dependent on replication licensing and S-phase CDK. The chromatin binding of the complex and that of Cdc45 are mutually dependent and both bindings require Xenopus Cut5, the yeast homolog of which interacts with Sld5. On replicating chromatin the complex interacts with Cdc45 and MCM, putative components of replication machinery. Electron microscopy further reveals that the complex has a ring-like structure. These results suggest that the complex plays an essential role in the elongation stage of DNA replication as well as the initiation stage.
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Aparicio,
Components and dynamics of DNA replication complexes in S. cerevisiae: redistribution of MCM proteins and Cdc45p during S phase.
1997, Pubmed
Aparicio,
Components and dynamics of DNA replication complexes in S. cerevisiae: redistribution of MCM proteins and Cdc45p during S phase.
1997,
Pubmed Araki,
Dpb11, which interacts with DNA polymerase II(epsilon) in Saccharomyces cerevisiae, has a dual role in S-phase progression and at a cell cycle checkpoint.
1995,
Pubmed Arata,
Electron microscopic observation of monomeric actin attached to a myosin head.
1998,
Pubmed Bell,
DNA replication in eukaryotic cells.
2002,
Pubmed Blow,
Replication licensing--defining the proliferative state?
2002,
Pubmed Blow,
Initiation of DNA replication in nuclei and purified DNA by a cell-free extract of Xenopus eggs.
1986,
Pubmed
,
Xenbase Gillespie,
Reconstitution of licensed replication origins on Xenopus sperm nuclei using purified proteins.
2001,
Pubmed
,
Xenbase Gulbis,
Structure of the C-terminal region of p21(WAF1/CIP1) complexed with human PCNA.
1996,
Pubmed Hashimoto,
Xenopus Cut5 is essential for a CDK-dependent process in the initiation of DNA replication.
2003,
Pubmed
,
Xenbase Homesley,
Mcm10 and the MCM2-7 complex interact to initiate DNA synthesis and to release replication factors from origins.
2000,
Pubmed Hutchison,
The control of DNA replication in a cell-free extract that recapitulates a basic cell cycle in vitro.
1988,
Pubmed
,
Xenbase Kamimura,
Sld2, which interacts with Dpb11 in Saccharomyces cerevisiae, is required for chromosomal DNA replication.
1998,
Pubmed Kamimura,
Sld3, which interacts with Cdc45 (Sld4), functions for chromosomal DNA replication in Saccharomyces cerevisiae.
2001,
Pubmed Kesti,
DNA polymerase epsilon catalytic domains are dispensable for DNA replication, DNA repair, and cell viability.
1999,
Pubmed Krishna,
Crystal structure of the eukaryotic DNA polymerase processivity factor PCNA.
1994,
Pubmed Kubota,
Determination of initiation of DNA replication before and after nuclear formation in Xenopus egg cell free extracts.
1993,
Pubmed
,
Xenbase Labib,
Uninterrupted MCM2-7 function required for DNA replication fork progression.
2000,
Pubmed Mäkiniemi,
BRCT domain-containing protein TopBP1 functions in DNA replication and damage response.
2001,
Pubmed Masai,
Cdc7 kinase complex: a key regulator in the initiation of DNA replication.
2002,
Pubmed Masuda,
CDK- and Cdc45-dependent priming of the MCM complex on chromatin during S-phase in Xenopus egg extracts: possible activation of MCM helicase by association with Cdc45.
2003,
Pubmed
,
Xenbase Masumoto,
S-Cdk-dependent phosphorylation of Sld2 essential for chromosomal DNA replication in budding yeast.
2002,
Pubmed Masumoto,
Dpb11 controls the association between DNA polymerases alpha and epsilon and the autonomously replicating sequence region of budding yeast.
2000,
Pubmed Mimura,
Xenopus Cdc45-dependent loading of DNA polymerase alpha onto chromatin under the control of S-phase Cdk.
1998,
Pubmed
,
Xenbase Mimura,
Central role for cdc45 in establishing an initiation complex of DNA replication in Xenopus egg extracts.
2000,
Pubmed
,
Xenbase Morrison,
A third essential DNA polymerase in S. cerevisiae.
1990,
Pubmed Nakajima,
SpSld3 is required for loading and maintenance of SpCdc45 on chromatin in DNA replication in fission yeast.
2002,
Pubmed Nishitani,
Control of DNA replication licensing in a cell cycle.
2002,
Pubmed
,
Xenbase Noguchi,
CDK phosphorylation of Drc1 regulates DNA replication in fission yeast.
2002,
Pubmed Ohya,
The DNA polymerase domain of pol(epsilon) is required for rapid, efficient, and highly accurate chromosomal DNA replication, telomere length maintenance, and normal cell senescence in Saccharomyces cerevisiae.
2002,
Pubmed Rowles,
Changes in association of the Xenopus origin recognition complex with chromatin on licensing of replication origins.
1999,
Pubmed
,
Xenbase Saka,
Fission yeast cut5+, required for S phase onset and M phase restraint, is identical to the radiation-damage repair gene rad4+.
1993,
Pubmed Tada,
Repression of origin assembly in metaphase depends on inhibition of RLF-B/Cdt1 by geminin.
2001,
Pubmed
,
Xenbase Takayama,
GINS, a novel multiprotein complex required for chromosomal DNA replication in budding yeast.
2003,
Pubmed Takisawa,
Eukaryotic DNA replication: from pre-replication complex to initiation complex.
2000,
Pubmed Tercero,
DNA synthesis at individual replication forks requires the essential initiation factor Cdc45p.
2000,
Pubmed Van Hatten,
The Xenopus Xmus101 protein is required for the recruitment of Cdc45 to origins of DNA replication.
2002,
Pubmed
,
Xenbase Waga,
Anatomy of a DNA replication fork revealed by reconstitution of SV40 DNA replication in vitro.
1994,
Pubmed Waga,
DNA polymerase epsilon is required for coordinated and efficient chromosomal DNA replication in Xenopus egg extracts.
2001,
Pubmed
,
Xenbase Walter,
Initiation of eukaryotic DNA replication: origin unwinding and sequential chromatin association of Cdc45, RPA, and DNA polymerase alpha.
2000,
Pubmed
,
Xenbase Wang,
DRC1, DNA replication and checkpoint protein 1, functions with DPB11 to control DNA replication and the S-phase checkpoint in Saccharomyces cerevisiae.
1999,
Pubmed Wohlschlegel,
Inhibition of eukaryotic DNA replication by geminin binding to Cdt1.
2000,
Pubmed
,
Xenbase Wohlschlegel,
Xenopus Mcm10 binds to origins of DNA replication after Mcm2-7 and stimulates origin binding of Cdc45.
2002,
Pubmed
,
Xenbase Yamamoto,
The Drosophila mus101 gene, which links DNA repair, replication and condensation of heterochromatin in mitosis, encodes a protein with seven BRCA1 C-terminus domains.
2000,
Pubmed Yamane,
A DNA damage-regulated BRCT-containing protein, TopBP1, is required for cell survival.
2002,
Pubmed
