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The small protein ARPP19 plays a dual role during oocyte meiosis resumption. In Xenopus, ARPP19 phosphorylation at S109 by PKA is necessary for maintaining oocytes arrested in prophase of the first meiotic division. Progesterone downregulates PKA, leading to the dephosphorylation of ARPP19 at S109. This initiates a transduction pathway ending with the activation of the universal inducer of M-phase, the kinase Cdk1. This last step depends on ARPP19 phosphorylation at S67 by the kinase Greatwall. Hence, phosphorylated by PKA at S109, ARPP19 restrains Cdk1 activation while when phosphorylated by Greatwall at S67, ARPP19 becomes an inducer of Cdk1 activation. Here, we investigate the functional interplay between S109 and S67-phosphorylations of ARPP19. We show that both PKA and Gwl phosphorylate ARPP19 independently of each other and that Cdk1 is not directly involved in regulating the biological activity of ARPP19. We also show that the phosphorylation of ARPP19 at S67 that activates Cdk1, is dominant over the inhibitory S109 phosphorylation. Therefore our results highlight the importance of timely synchronizing ARPP19 phosphorylations at S109 and S67 to fully activate Cdk1.
,
Corrigendum.
2018,
Pubmed Andrade,
ARPP-16 Is a Striatal-Enriched Inhibitor of Protein Phosphatase 2A Regulated by Microtubule-Associated Serine/Threonine Kinase 3 (Mast 3 Kinase).
2017,
Pubmed Bontron,
Yeast endosulfines control entry into quiescence and chronological life span by inhibiting protein phosphatase 2A.
2013,
Pubmed
,
Xenbase Bosch,
The PR55 and PR65 subunits of protein phosphatase 2A from Xenopus laevis. molecular cloning and developmental regulation of expression.
1995,
Pubmed
,
Xenbase Burgess,
Loss of human Greatwall results in G2 arrest and multiple mitotic defects due to deregulation of the cyclin B-Cdc2/PP2A balance.
2010,
Pubmed Castilho,
The M phase kinase Greatwall (Gwl) promotes inactivation of PP2A/B55delta, a phosphatase directed against CDK phosphosites.
2009,
Pubmed
,
Xenbase Daar,
Inhibition of mos-induced oocyte maturation by protein kinase A.
1993,
Pubmed
,
Xenbase Daldello,
Control of Cdc6 accumulation by Cdk1 and MAPK is essential for completion of oocyte meiotic divisions in Xenopus.
2015,
Pubmed
,
Xenbase De Smedt,
Thr-161 phosphorylation of monomeric Cdc2. Regulation by protein phosphatase 2C in Xenopus oocytes.
2002,
Pubmed
,
Xenbase Duckworth,
G2 arrest in Xenopus oocytes depends on phosphorylation of cdc25 by protein kinase A.
2002,
Pubmed
,
Xenbase Dulubova,
ARPP-16/ARPP-19: a highly conserved family of cAMP-regulated phosphoproteins.
2001,
Pubmed Dupré,
Phosphorylation of ARPP19 by protein kinase A prevents meiosis resumption in Xenopus oocytes.
2014,
Pubmed
,
Xenbase Dupré,
The phosphorylation of ARPP19 by Greatwall renders the auto-amplification of MPF independently of PKA in Xenopus oocytes.
2013,
Pubmed
,
Xenbase Eyers,
Regulation of the G(2)/M transition in Xenopus oocytes by the cAMP-dependent protein kinase.
2005,
Pubmed
,
Xenbase Frank-Vaillant,
Two distinct mechanisms control the accumulation of cyclin B1 and Mos in Xenopus oocytes in response to progesterone.
1999,
Pubmed
,
Xenbase Gaffré,
A critical balance between Cyclin B synthesis and Myt1 activity controls meiosis entry in Xenopus oocytes.
2011,
Pubmed
,
Xenbase Gharbi-Ayachi,
The substrate of Greatwall kinase, Arpp19, controls mitosis by inhibiting protein phosphatase 2A.
2010,
Pubmed
,
Xenbase Goris,
Okadaic acid, a specific protein phosphatase inhibitor, induces maturation and MPF formation in Xenopus laevis oocytes.
1989,
Pubmed
,
Xenbase Haccard,
Oocyte maturation, Mos and cyclins--a matter of synthesis: two functionally redundant ways to induce meiotic maturation.
2006,
Pubmed Hégarat,
Bistability of mitotic entry and exit switches during open mitosis in mammalian cells.
2016,
Pubmed Huchon,
The pure inhibitor of cAMP-dependent protein kinase initiates Xenopus laevis meiotic maturation. A 4-step scheme for meiotic maturation.
1981,
Pubmed
,
Xenbase Jiang,
ARPP-19 promotes proliferation and metastasis of human glioma.
2016,
Pubmed Juanes,
Budding yeast greatwall and endosulfines control activity and spatial regulation of PP2A(Cdc55) for timely mitotic progression.
2013,
Pubmed
,
Xenbase Kim,
Bypassing the Greatwall-Endosulfine pathway: plasticity of a pivotal cell-cycle regulatory module in Drosophila melanogaster and Caenorhabditis elegans.
2012,
Pubmed Kishimoto,
Entry into mitosis: a solution to the decades-long enigma of MPF.
2015,
Pubmed Labandera,
The mitotic PP2A regulator ENSA/ARPP-19 is remarkably conserved across plants and most eukaryotes.
2015,
Pubmed Lindqvist,
The decision to enter mitosis: feedback and redundancy in the mitotic entry network.
2009,
Pubmed Lorca,
Constant regulation of both the MPF amplification loop and the Greatwall-PP2A pathway is required for metaphase II arrest and correct entry into the first embryonic cell cycle.
2010,
Pubmed
,
Xenbase Maller,
Progesterone-stimulated meiotic cell division in Xenopus oocytes. Induction by regulatory subunit and inhibition by catalytic subunit of adenosine 3':5'-monophosphate-dependent protein kinase.
1977,
Pubmed
,
Xenbase Maller,
Early effect of progesterone on levels of cyclic adenosine 3':5'-monophosphate in Xenopus oocytes.
1979,
Pubmed
,
Xenbase Matten,
Protein kinase A acts at multiple points to inhibit Xenopus oocyte maturation.
1994,
Pubmed
,
Xenbase Mochida,
Regulation of α-endosulfine, an inhibitor of protein phosphatase 2A, by multisite phosphorylation.
2014,
Pubmed
,
Xenbase Mochida,
Regulated activity of PP2A-B55 delta is crucial for controlling entry into and exit from mitosis in Xenopus egg extracts.
2009,
Pubmed
,
Xenbase Mochida,
Greatwall phosphorylates an inhibitor of protein phosphatase 2A that is essential for mitosis.
2010,
Pubmed
,
Xenbase Mulner,
Cyclic AMP synthesis in Xenopus laevis oocytes: inhibition by progesterone.
1979,
Pubmed
,
Xenbase Nader,
Release from Xenopus oocyte prophase I meiotic arrest is independent of a decrease in cAMP levels or PKA activity.
2016,
Pubmed
,
Xenbase Okumura,
Cyclin B-Cdk1 inhibits protein phosphatase PP2A-B55 via a Greatwall kinase-independent mechanism.
2014,
Pubmed Ozon,
Roles of cyclic AMP and calcium in maturation of Xenopus laevis oocytes.
1979,
Pubmed
,
Xenbase Rangone,
Suppression of scant identifies Endos as a substrate of greatwall kinase and a negative regulator of protein phosphatase 2A in mitosis.
2011,
Pubmed Rime,
Activation of p34cdc2 kinase by cyclin is negatively regulated by cyclic amp-dependent protein kinase in Xenopus oocytes.
1992,
Pubmed
,
Xenbase Schmitz,
Live-cell imaging RNAi screen identifies PP2A-B55alpha and importin-beta1 as key mitotic exit regulators in human cells.
2010,
Pubmed Smith,
The induction of oocyte maturation: transmembrane signaling events and regulation of the cell cycle.
1989,
Pubmed Studier,
Protein production by auto-induction in high density shaking cultures.
2005,
Pubmed Talarek,
Initiation of the TORC1-regulated G0 program requires Igo1/2, which license specific mRNAs to evade degradation via the 5'-3' mRNA decay pathway.
2010,
Pubmed Talarek,
Quantification of mRNA stability of stress-responsive yeast genes following conditional excision of open reading frames.
2013,
Pubmed Vigneron,
The master Greatwall kinase, a critical regulator of mitosis and meiosis.
2016,
Pubmed
,
Xenbase Vigneron,
Greatwall maintains mitosis through regulation of PP2A.
2009,
Pubmed
,
Xenbase Wang,
Protein kinase A(PKA)-restrictive and PKA-permissive phases of oocyte maturation.
2006,
Pubmed
,
Xenbase Wang,
Progesterone inhibits protein kinase A (PKA) in Xenopus oocytes: demonstration of endogenous PKA activities using an expressed substrate.
2004,
Pubmed
,
Xenbase White,
MicroRNA-320 induces neurite outgrowth by targeting ARPP-19.
2012,
Pubmed Williams,
Greatwall-phosphorylated Endosulfine is both an inhibitor and a substrate of PP2A-B55 heterotrimers.
2014,
Pubmed Yu,
Greatwall kinase participates in the Cdc2 autoregulatory loop in Xenopus egg extracts.
2006,
Pubmed
,
Xenbase Yu,
Greatwall kinase: a nuclear protein required for proper chromosome condensation and mitotic progression in Drosophila.
2004,
Pubmed
