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EMBO J
2017 Nov 02;3621:3212-3231. doi: 10.15252/embj.201796585.
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Initiation of DNA replication requires actin dynamics and formin activity.
Parisis N, Krasinska L, Harker B, Urbach S, Rossignol M, Camasses A, Dewar J, Morin N, Fisher D.
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Nuclear actin regulates transcriptional programmes in a manner dependent on its levels and polymerisation state. This dynamics is determined by the balance of nucleocytoplasmic shuttling, formin- and redox-dependent filament polymerisation. Here, using Xenopus egg extracts and human somatic cells, we show that actin dynamics and formins are essential for DNA replication. In proliferating cells, formin inhibition abolishes nuclear transport and initiation of DNA replication, as well as general transcription. In replicating nuclei from transcriptionally silent Xenopus egg extracts, we identified numerous actin regulators, and disruption of actin dynamics abrogates nuclear transport, preventing NLS (nuclear localisation signal)-cargo release from RanGTP-importin complexes. Nuclear formin activity is further required to promote loading of cyclin-dependent kinase (CDK) and proliferating cell nuclear antigen (PCNA) onto chromatin, as well as initiation and elongation of DNA replication. Therefore, actin dynamics and formins control DNA replication by multiple direct and indirect mechanisms.
Allen,
Visualization of the nucleus and nuclear envelope in situ by SEM in tissue culture cells.
2007, Pubmed,
Xenbase
Allen,
Visualization of the nucleus and nuclear envelope in situ by SEM in tissue culture cells.
2007,
Pubmed
,
Xenbase Arias,
Initiation of DNA replication in Xenopus egg extracts.
2004,
Pubmed
,
Xenbase Assoian,
Cell anchorage and the cytoskeleton as partners in growth factor dependent cell cycle progression.
1997,
Pubmed Baarlink,
Nuclear actin network assembly by formins regulates the SRF coactivator MAL.
2013,
Pubmed Bai,
Importin β-dependent nuclear import of TopBP1 in ATR-Chk1 checkpoint in Xenopus egg extracts.
2014,
Pubmed
,
Xenbase Blow,
Initiation of DNA replication in nuclei and purified DNA by a cell-free extract of Xenopus eggs.
1986,
Pubmed
,
Xenbase Bompard,
Subgroup II PAK-mediated phosphorylation regulates Ran activity during mitosis.
2010,
Pubmed
,
Xenbase Bompard,
Involvement of Rac in actin cytoskeleton rearrangements induced by MIM-B.
2005,
Pubmed Burgess,
Quantitative live imaging of endogenous DNA replication in mammalian cells.
2012,
Pubmed Clark,
An actin filament matrix in hand-isolated nuclei of X. laevis oocytes.
1979,
Pubmed
,
Xenbase Conaway,
The INO80 chromatin remodeling complex in transcription, replication and repair.
2009,
Pubmed Cox,
DNA replication in cell-free extracts from Xenopus eggs is prevented by disrupting nuclear envelope function.
1992,
Pubmed
,
Xenbase de Lanerolle,
Nuclear actin and myosins at a glance.
2012,
Pubmed Densham,
MST kinases monitor actin cytoskeletal integrity and signal via c-Jun N-terminal kinase stress-activated kinase to regulate p21Waf1/Cip1 stability.
2009,
Pubmed Dopie,
Genome-wide RNAi screen for nuclear actin reveals a network of cofilin regulators.
2015,
Pubmed Dopie,
Active maintenance of nuclear actin by importin 9 supports transcription.
2012,
Pubmed Echalier,
An integrated chemical biology approach provides insight into Cdk2 functional redundancy and inhibitor sensitivity.
2012,
Pubmed
,
Xenbase Feric,
A nuclear F-actin scaffold stabilizes ribonucleoprotein droplets against gravity in large cells.
2013,
Pubmed
,
Xenbase Finlay,
Inhibition of in vitro nuclear transport by a lectin that binds to nuclear pores.
1987,
Pubmed
,
Xenbase Gauvin,
Isoform-selective chemical inhibition of mDia-mediated actin assembly.
2009,
Pubmed Gounon,
Involvement of contractile proteins in the changes in consistency of oocyte nucleoplasm of the newt Pleurodeles waltlii.
1981,
Pubmed Gray,
Exploiting chemical libraries, structure, and genomics in the search for kinase inhibitors.
1998,
Pubmed Grosse,
To be or not to be assembled: progressing into nuclear actin filaments.
2013,
Pubmed Hetzer,
Pushing the envelope: structure, function, and dynamics of the nuclear periphery.
2005,
Pubmed Hofmann,
Actin is part of pre-initiation complexes and is necessary for transcription by RNA polymerase II.
2004,
Pubmed Hu,
A role for beta-actin in RNA polymerase III transcription.
2004,
Pubmed Huang,
Coronin 2A mediates actin-dependent de-repression of inflammatory response genes.
2011,
Pubmed Huang,
Systematic and integrative analysis of large gene lists using DAVID bioinformatics resources.
2009,
Pubmed Huet,
Nuclear actin levels as an important transcriptional switch.
2012,
Pubmed Iida,
Heat shock induction of intranuclear actin rods in cultured mammalian cells.
1986,
Pubmed Isogai,
Proteomic analyses uncover a new function and mode of action for mouse homolog of Diaphanous 2 (mDia2).
2015,
Pubmed Isogai,
Quantitative Proteomics Illuminates a Functional Interaction between mDia2 and the Proteasome.
2016,
Pubmed Johnson,
Stimulation of in vivo nuclear transport dynamics of actin and its co-factors IQGAP1 and Rac1 in response to DNA replication stress.
2013,
Pubmed Kalendová,
Nuclear actin filaments recruit cofilin and actin-related protein 3, and their formation is connected with a mitotic block.
2014,
Pubmed Kapoor,
Evidence for monomeric actin function in INO80 chromatin remodeling.
2013,
Pubmed Khoudoli,
Temporal profiling of the chromatin proteome reveals system-wide responses to replication inhibition.
2008,
Pubmed
,
Xenbase Kim,
Lys-110 is essential for targeting PCNA to replication and repair foci, and the K110A mutant activates apoptosis.
2008,
Pubmed Kiseleva,
Actin- and protein-4.1-containing filaments link nuclear pore complexes to subnuclear organelles in Xenopus oocyte nuclei.
2004,
Pubmed
,
Xenbase Krasinska,
Replication initiation complex formation in the absence of nuclear function in Xenopus.
2009,
Pubmed
,
Xenbase Krauss,
Nuclear actin and protein 4.1: essential interactions during nuclear assembly in vitro.
2003,
Pubmed
,
Xenbase Kurat,
Chromatin Controls DNA Replication Origin Selection, Lagging-Strand Synthesis, and Replication Fork Rates.
2017,
Pubmed Labib,
How do Cdc7 and cyclin-dependent kinases trigger the initiation of chromosome replication in eukaryotic cells?
2010,
Pubmed Lee,
MsrB1 and MICALs regulate actin assembly and macrophage function via reversible stereoselective methionine oxidation.
2013,
Pubmed Lee,
Structural basis for nuclear import complex dissociation by RanGTP.
2005,
Pubmed Lowe,
Selectivity mechanism of the nuclear pore complex characterized by single cargo tracking.
2010,
Pubmed Lundquist,
Redox modification of nuclear actin by MICAL-2 regulates SRF signaling.
2014,
Pubmed Martinez Molina,
Monitoring drug target engagement in cells and tissues using the cellular thermal shift assay.
2013,
Pubmed McDonald,
Nucleoplasmic beta-actin exists in a dynamic equilibrium between low-mobility polymeric species and rapidly diffusing populations.
2006,
Pubmed Méchali,
DNA synthesis in a cell-free system from Xenopus eggs: priming and elongation on single-stranded DNA in vitro.
1982,
Pubmed
,
Xenbase Miyamoto,
Nuclear actin polymerization is required for transcriptional reprogramming of Oct4 by oocytes.
2011,
Pubmed
,
Xenbase Miyamoto,
Nuclear Wave1 is required for reprogramming transcription in oocytes and for normal development.
2013,
Pubmed
,
Xenbase Morel,
Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) induces rheumatoid arthritis synovial fibroblast proliferation through mitogen-activated protein kinases and phosphatidylinositol 3-kinase/Akt.
2005,
Pubmed Németh,
Disruption of the actin cytoskeleton results in nuclear factor-kappaB activation and inflammatory mediator production in cultured human intestinal epithelial cells.
2004,
Pubmed Obrdlik,
The F-actin severing protein cofilin-1 is required for RNA polymerase II transcription elongation.
2011,
Pubmed Parisis,
Initiation of DNA replication requires actin dynamics and formin activity.
2017,
Pubmed
,
Xenbase Philimonenko,
Nuclear actin and myosin I are required for RNA polymerase I transcription.
2004,
Pubmed Plessner,
Nuclear F-actin formation and reorganization upon cell spreading.
2015,
Pubmed Poulet,
NucleusJ: an ImageJ plugin for quantifying 3D images of interphase nuclei.
2015,
Pubmed Rando,
Phosphatidylinositol-dependent actin filament binding by the SWI/SNF-like BAF chromatin remodeling complex.
2002,
Pubmed Riedl,
Lifeact: a versatile marker to visualize F-actin.
2008,
Pubmed Rizvi,
Identification and characterization of a small molecule inhibitor of formin-mediated actin assembly.
2009,
Pubmed Sanger,
Reversible translocation of cytoplasmic actin into the nucleus caused by dimethyl sulfoxide.
1980,
Pubmed Schindelin,
Fiji: an open-source platform for biological-image analysis.
2012,
Pubmed Schliwa,
Action of cytochalasin D on cytoskeletal networks.
1982,
Pubmed Serebryannyy,
Persistent nuclear actin filaments inhibit transcription by RNA polymerase II.
2016,
Pubmed Sero,
Cell shape and the microenvironment regulate nuclear translocation of NF-κB in breast epithelial and tumor cells.
2015,
Pubmed Shevchenko,
In-gel digestion for mass spectrometric characterization of proteins and proteomes.
2006,
Pubmed Small,
Visualising the actin cytoskeleton.
1999,
Pubmed Soderholm,
Importazole, a small molecule inhibitor of the transport receptor importin-β.
2011,
Pubmed
,
Xenbase Spencer,
Depletion of nuclear actin is a key mediator of quiescence in epithelial cells.
2011,
Pubmed Stüven,
Exportin 6: a novel nuclear export receptor that is specific for profilin.actin complexes.
2003,
Pubmed Talcott,
The nuclear import of RCC1 requires a specific nuclear localization sequence receptor, karyopherin alpha3/Qip.
2000,
Pubmed
,
Xenbase Tutter,
Chromosomal DNA replication in a soluble cell-free system derived from Xenopus eggs.
2006,
Pubmed
,
Xenbase Vartiainen,
Nuclear actin regulates dynamic subcellular localization and activity of the SRF cofactor MAL.
2007,
Pubmed Walter,
Regulated chromosomal DNA replication in the absence of a nucleus.
1998,
Pubmed
,
Xenbase Wu,
Regulation of RNA-polymerase-II-dependent transcription by N-WASP and its nuclear-binding partners.
2006,
Pubmed Yamada,
Identification and functional characterization of FMN2, a regulator of the cyclin-dependent kinase inhibitor p21.
2013,
Pubmed Yoo,
A novel role of the actin-nucleating Arp2/3 complex in the regulation of RNA polymerase II-dependent transcription.
2007,
Pubmed
