Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Wilehm Roux Arch Dev Biol
1978 Sep 01;1853:235-248. doi: 10.1007/BF00848354.
Show Gene links
Show Anatomy links
Retention of the differentiated state by larvalXenopus liver cells in primary culture.
Wahli W, Abraham I, Weber R.
???displayArticle.abstract???
Electron microscopic analysis of primary cultures derived from larvalXenopus liver has shown that these cells, although they form only two-dimensional aggregates, retain and presumably also develop structural characteristics typical of liver parenchyma cells, such as bile canaliculi with microvilli and epithelial junctional complexes. As judged from structural criteria, primary cultures contain 80-90% hepatocytes. In contrast to the intact tissue, primary cultures showed excessive development of microfilaments, however.Incorporation of labeled amino acids has revealed further that the capacity for protein synthesis is maintained in culture and that synthesis of liverspecific protein albumin is maintained in vitro, even in liver cultures derived from thyrostatic tadpoles. This latter result suggests that initiation of albumin synthesis in the larval liver is probably not dependent upon thyroid hormones but rather reflects the protodifferentiated state of this tissue.
Berridge,
Translation of Xenopus liver messenger RNA in Xenopus oocytes: vitellogenin synthesis and conversion to yolk platelet proteins.
1976, Pubmed,
Xenbase
Berridge,
Translation of Xenopus liver messenger RNA in Xenopus oocytes: vitellogenin synthesis and conversion to yolk platelet proteins.
1976,
Pubmed
,
Xenbase Berridge,
Characterization of polysomes from Xenopus liver synthesizing vitellogenin and translation of vitellogenin and albumin messenger RNA's in vitro.
1976,
Pubmed
,
Xenbase Bisbee,
Albumin phylogeny for clawed frogs (Xenopus).
1977,
Pubmed
,
Xenbase Bragina,
Formation of bundles of microfilaments during spreading of fibroblasts on the substrate.
1976,
Pubmed Chen,
Patterns and metamorphic changes of serum proteins in amphibia.
1970,
Pubmed DAVIS,
DISC ELECTROPHORESIS. II. METHOD AND APPLICATION TO HUMAN SERUM PROTEINS.
1964,
Pubmed Dipasquale,
Locomotion of epithelial cells. Factors involved in extension of the leading edge.
1975,
Pubmed Fairbanks,
Electrophoretic analysis of the major polypeptides of the human erythrocyte membrane.
1971,
Pubmed Goldman,
The distribution of actin in non-muscle cells. The use of actin antibody in the localization of actin within the microfilament bundles of mouse 3T3 cells.
1975,
Pubmed Grieninger,
Snythesis and differentiation of plasma proteins in cultured embryonic chicken liver cells: a system for study of regulation of protein synthesis.
1975,
Pubmed Jeejeebhoy,
Albumin, fibrinogen and transferrin synthesis in isolated rat hepatocyte suspensions. A model for the study of plasma protein synthesis.
1975,
Pubmed Kistler,
A combined biochemical and morphometric study on tissue changes in Xenopus larvae during induced metamorphosis.
1975,
Pubmed
,
Xenbase Laemmli,
Maturation of the head of bacteriophage T4. I. DNA packaging events.
1973,
Pubmed Laskey,
Quantitative film detection of 3H and 14C in polyacrylamide gels by fluorography.
1975,
Pubmed Lemonnier,
[Albumin synthesis during culture of human hepatic parenchyma].
1974,
Pubmed Perissel,
[Ultrastructural study of hepatocytes of newborn rats in established monolayer cultures].
1973,
Pubmed REYNOLDS,
The use of lead citrate at high pH as an electron-opaque stain in electron microscopy.
1963,
Pubmed RUTTER,
CONTROL OF SPECIFIC SYNTHESIS IN THE DEVELOPING PANCREAS.
1964,
Pubmed Travis,
Isolation of albumin from whole human plasma and fractionation of albumin-depleted plasma.
1976,
Pubmed Wahli,
Factors promoting the establishment of primary cultures of liver cells fromXenopus larvae.
1977,
Pubmed
,
Xenbase Wanson,
Adult rat hepatocytes in primary monolayer culture. Ultrastructural characteristics of intercellular contacts and cell membrane differentiations.
1977,
Pubmed Weeke,
A manual of quantitative immunoelectrophoresis. Methods and applications. 1. General remarks on principles, equipment, reagents and procedures.
1973,
Pubmed Weignand,
Intact endoplasmic reticulum and albumin synthesis in rat liver cell suspensions.
1971,
Pubmed
