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Cold Spring Harb Protoc
2018 May 01;20185:. doi: 10.1101/pdb.prot097030.
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Fluorescence In Situ Hybridization of Cryosectioned Xenopus Oocytes.
Neil CR, Mowry K.
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Xenopus laevis oocytes are widely used to study mechanisms of RNA function and biogenesis. While the large size of Xenopus oocytes is amenable to both biochemical and imaging approaches, the relative opacity of the yolk-rich cytoplasm has limited high-resolution imaging of endogenous RNAs. Here, we present a protocol that combines multi-probe fluorescence in situ hybridization with cryosectioning to provide a highly sensitive means of imaging endogenous oocyte RNAs.
Agricola,
Two-color fluorescence in situ hybridization using chromogenic substrates in Xenopus.
2016, Pubmed,
Xenbase
Agricola,
Two-color fluorescence in situ hybridization using chromogenic substrates in Xenopus.
2016,
Pubmed
,
Xenbase Bilinski,
Electron microscopy, immunostaining, cytoskeleton visualization, in situ hybridization, and three-dimensional reconstruction of Xenopus oocytes.
2010,
Pubmed
,
Xenbase Colegrove-Otero,
The Xenopus ELAV protein ElrB represses Vg1 mRNA translation during oogenesis.
2005,
Pubmed
,
Xenbase Dumont,
Oogenesis in Xenopus laevis (Daudin). I. Stages of oocyte development in laboratory maintained animals.
1972,
Pubmed
,
Xenbase Gagnon,
Directional transport is mediated by a Dynein-dependent step in an RNA localization pathway.
2013,
Pubmed
,
Xenbase Gall,
Assembly of the nuclear transcription and processing machinery: Cajal bodies (coiled bodies) and transcriptosomes.
1999,
Pubmed
,
Xenbase Hake,
CPEB is a specificity factor that mediates cytoplasmic polyadenylation during Xenopus oocyte maturation.
1994,
Pubmed
,
Xenbase Hudson,
Xpat, a gene expressed specifically in germ plasm and primordial germ cells of Xenopus laevis.
1998,
Pubmed
,
Xenbase Kloc,
Potential structural role of non-coding and coding RNAs in the organization of the cytoskeleton at the vegetal cortex of Xenopus oocytes.
2005,
Pubmed
,
Xenbase McGrew,
Poly(A) elongation during Xenopus oocyte maturation is required for translational recruitment and is mediated by a short sequence element.
1989,
Pubmed
,
Xenbase Melton,
Translocation of a localized maternal mRNA to the vegetal pole of Xenopus oocytes.
,
Pubmed
,
Xenbase Messitt,
Multiple kinesin motors coordinate cytoplasmic RNA transport on a subpopulation of microtubules in Xenopus oocytes.
2008,
Pubmed
,
Xenbase Mowry,
Vegetal messenger RNA localization directed by a 340-nt RNA sequence element in Xenopus oocytes.
1992,
Pubmed
,
Xenbase Newman,
Isolation of Xenopus Oocytes.
2018,
Pubmed
,
Xenbase Raj,
Imaging individual mRNA molecules using multiple singly labeled probes.
2008,
Pubmed Weeks,
A maternal mRNA localized to the vegetal hemisphere in Xenopus eggs codes for a growth factor related to TGF-beta.
1987,
Pubmed
,
Xenbase Yoon,
Xenopus Staufen is a component of a ribonucleoprotein complex containing Vg1 RNA and kinesin.
2004,
Pubmed
,
Xenbase
