Cell 2003 Apr 04;1131:101-13. doi: 10.1016/s0092-8674(03)00232-0.

Tome-1, a trigger of mitotic entry, is degraded during G1 via the APC.

???displayArticle.abstract???
Entry into mitosis requires the activation of cdk1/cyclin B, while mitotic exit is achieved when the same kinase activity decreases, as cyclin B is degraded. Cyclin B proteolysis is mediated by the anaphase promoting complex, or APC, an E3 ligase that is active at anaphase in mitosis through G1. We have identified a G1 substrate of the APC that we have termed Tome-1, for trigger of mitotic entry. Tome-1 is a cytosolic protein required for proper activation of cdk1/cyclin B and mitotic entry. Tome-1 associates with Skp-1 and is required for degradation of the cdk1 inhibitory tyrosine kinase wee1; Tome-1 therefore appears to be acting as part of an SCF-type E3 for wee1. Degradation of Tome-1 during G1 allows for wee 1 accumulation during interphase, thereby providing a critical link between the APC and SCF pathways in regulation of cdk1/cyclin B activity and thus mitotic entry and exit.

???displayArticle.pubmedLink??? 12679038
???displayArticle.link??? Cell
???displayArticle.grants??? [+]

Species referenced: Xenopus
Genes referenced: cdca3 cdk1 wee1