Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
???displayArticle.abstract???
In situ hybridization performed using whole fixed embryos provides accurate and detailed visualization of gene expression patterns. These patterns are useful for investigating spatial patterns of gene expression in normally developing embryos but can also be useful in investigating the effects of genetic or environmental changes on expression of genetic markers characteristic of particular tissues, organs, or genetic pathways. Our lab's protocol for whole-mount in situ hybridization is presented.
Fig. 1
Visualization of paired-type homeobox gene expression in the developing headskeleton of tailbud stage Xenopus laevis embryos. Whole-mount in situ hybridization was performed on embryos fixed at stages 28–30 (panels a–d) or 34–36 (panels e–h) using antisense riboprobes for alx1 (a, e), alx4 (b, f), prrx1 (c, g), or prrx2 (d, h). Specific stages are indicated in the lower right corner of each panel. Gene-specific antisense riboprobes were synthesized by in vitro transcription (Ambion MEGAscript kit) using specific cDNAs generated by 3′-RACE (Clontech SMARTer RACE Kit) using total RNA isolated from mixed neurula stage embryos using degenerate primers encoding VQVWFQN (5′-GTN CAR GTN TGG TTY CAR AAY-3′). Anatomical features are indicated in panel G: C—cement gland; E—eye; F—frontonasal process; Op—olfactory placode/pit; Ov—otic vesicle; P—pharyngeal arch
alx4 (ALX homeobox 4) gene expression in Xenopus laevis embryo, head region only, assayed via in situ hybridization, NF stage 28, lateral view, anteriorleft, dorsal up.
