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Nat Commun
2018 Aug 28;91:3491. doi: 10.1038/s41467-018-05924-9.
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TBC1d24-ephrinB2 interaction regulates contact inhibition of locomotion in neural crest cell migration.
Yoon J, Hwang YS, Lee M, Sun J, Cho HJ, Knapik L, Daar IO.
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Although Eph-ephrin signalling has been implicated in the migration of cranial neural crest (CNC) cells, it is still unclear how ephrinB transduces signals regulating this event. We provide evidence that TBC1d24, a putative Rab35-GTPase activating protein (Rab35 GAP), complexes with ephrinB2 via the scaffold Dishevelled (Dsh) and mediates a signal affecting contact inhibition of locomotion (CIL) in CNC cells. Moreover, we found that, in migrating CNC, the interaction between ephrinB2 and TBC1d24 negatively regulates E-cadherin recycling in these cells via Rab35. Upon engagement of the cognate Eph receptor, ephrinB2 is tyrosine phosphorylated, which disrupts the ephrinB2/Dsh/TBC1d24 complex. The dissolution of this complex leads to increasing E-cadherin levels at the plasma membrane, resulting in loss of CIL and disrupted CNC migration. Our results indicate that TBC1d24 is a critical player in ephrinB2 control of CNC cell migration via CIL.
Abbruzzese,
ADAM13 cleavage of cadherin-11 promotes CNC migration independently of the homophilic binding site.
2016, Pubmed,
Xenbase
Abbruzzese,
ADAM13 cleavage of cadherin-11 promotes CNC migration independently of the homophilic binding site.
2016,
Pubmed
,
Xenbase Adams,
The cytoplasmic domain of the ligand ephrinB2 is required for vascular morphogenesis but not cranial neural crest migration.
2001,
Pubmed Allaire,
Interplay between Rab35 and Arf6 controls cargo recycling to coordinate cell adhesion and migration.
2013,
Pubmed Arboleda-Estudillo,
Movement directionality in collective migration of germ layer progenitors.
2010,
Pubmed Astin,
Competition amongst Eph receptors regulates contact inhibition of locomotion and invasiveness in prostate cancer cells.
2010,
Pubmed Bahm,
PDGF controls contact inhibition of locomotion by regulating N-cadherin during neural crest migration.
2017,
Pubmed
,
Xenbase Barriga,
Tissue stiffening coordinates morphogenesis by triggering collective cell migration in vivo.
2018,
Pubmed
,
Xenbase Becker,
Cadherin-11 mediates contact inhibition of locomotion during Xenopus neural crest cell migration.
2013,
Pubmed
,
Xenbase Borchers,
Xenopus cadherin-11 restrains cranial neural crest migration and influences neural crest specification.
2001,
Pubmed
,
Xenbase Brückner,
Tyrosine phosphorylation of transmembrane ligands for Eph receptors.
1997,
Pubmed Bush,
Eph/ephrin signaling: genetic, phosphoproteomic, and transcriptomic approaches.
2012,
Pubmed Campeau,
The genetic basis of DOORS syndrome: an exome-sequencing study.
2014,
Pubmed Carmona-Fontaine,
Complement fragment C3a controls mutual cell attraction during collective cell migration.
2011,
Pubmed
,
Xenbase Charrasse,
Rab35 regulates cadherin-mediated adherens junction formation and myoblast fusion.
2013,
Pubmed Cho,
EphrinB1 promotes cancer cell migration and invasion through the interaction with RhoGDI1.
2018,
Pubmed Cho,
EphrinB1 interacts with CNK1 and promotes cell migration through c-Jun N-terminal kinase (JNK) activation.
2014,
Pubmed Chong,
Fibroblast growth factor receptor-mediated rescue of x-ephrin B1-induced cell dissociation in Xenopus embryos.
2000,
Pubmed
,
Xenbase Coulthard,
Eph/Ephrin signaling in injury and inflammation.
2012,
Pubmed Cousin,
Cadherins function during the collective cell migration of Xenopus Cranial Neural Crest cells: revisiting the role of E-cadherin.
2017,
Pubmed
,
Xenbase Cousin,
Cranial Neural Crest Explants.
2018,
Pubmed
,
Xenbase Daar,
Non-SH2/PDZ reverse signaling by ephrins.
2012,
Pubmed Davy,
Ephrin-B2 forward signaling regulates somite patterning and neural crest cell development.
2007,
Pubmed Davy,
Ephrin-B1 forward and reverse signaling are required during mouse development.
2004,
Pubmed De Calisto,
Essential role of non-canonical Wnt signalling in neural crest migration.
2005,
Pubmed
,
Xenbase Desclozeaux,
Active Rab11 and functional recycling endosome are required for E-cadherin trafficking and lumen formation during epithelial morphogenesis.
2008,
Pubmed Falace,
TBC1D24 regulates neuronal migration and maturation through modulation of the ARF6-dependent pathway.
2014,
Pubmed Falace,
TBC1D24, an ARF6-interacting protein, is mutated in familial infantile myoclonic epilepsy.
2010,
Pubmed Gammill,
Neuropilin 2/semaphorin 3F signaling is essential for cranial neural crest migration and trigeminal ganglion condensation.
2007,
Pubmed Guven,
TBC1D24 truncating mutation resulting in severe neurodegeneration.
2013,
Pubmed Harland,
In situ hybridization: an improved whole-mount method for Xenopus embryos.
1991,
Pubmed
,
Xenbase Holland,
Juxtamembrane tyrosine residues couple the Eph family receptor EphB2/Nuk to specific SH2 domain proteins in neuronal cells.
1997,
Pubmed Huang,
E-cadherin is required for cranial neural crest migration in Xenopus laevis.
2016,
Pubmed
,
Xenbase Hwang,
A frog's view of EphrinB signaling.
2017,
Pubmed
,
Xenbase Ji,
EphrinB2 affects apical constriction in Xenopus embryos and is regulated by ADAM10 and flotillin-1.
2014,
Pubmed
,
Xenbase Kashef,
Cadherin-11 regulates protrusive activity in Xenopus cranial neural crest cells upstream of Trio and the small GTPases.
2009,
Pubmed
,
Xenbase Krull,
Interactions of Eph-related receptors and ligands confer rostrocaudal pattern to trunk neural crest migration.
1997,
Pubmed Kuriyama,
Molecular analysis of neural crest migration.
2008,
Pubmed
,
Xenbase Langhe,
Cadherin-11 localizes to focal adhesions and promotes cell-substrate adhesion.
2016,
Pubmed
,
Xenbase Lee,
Fibroblast growth factor receptor-induced phosphorylation of ephrinB1 modulates its interaction with Dishevelled.
2009,
Pubmed
,
Xenbase Lee,
Dishevelled mediates ephrinB1 signalling in the eye field through the planar cell polarity pathway.
2006,
Pubmed
,
Xenbase Li,
E-cadherin plays an essential role in collective directional migration of large epithelial sheets.
2012,
Pubmed Lu,
Ephrin-B reverse signaling is mediated by a novel PDZ-RGS protein and selectively inhibits G protein-coupled chemoattraction.
2001,
Pubmed
,
Xenbase Mao,
Preliminary identification of potential PDZ-domain proteins downstream of ephrin B2 during osteoclast differentiation of RAW264.7 cells.
2011,
Pubmed Matthews,
Directional migration of neural crest cells in vivo is regulated by Syndecan-4/Rac1 and non-canonical Wnt signaling/RhoA.
2008,
Pubmed
,
Xenbase McCusker,
Extracellular cleavage of cadherin-11 by ADAM metalloproteases is essential for Xenopus cranial neural crest cell migration.
2009,
Pubmed
,
Xenbase McLennan,
Vascular endothelial growth factor (VEGF) regulates cranial neural crest migration in vivo.
2010,
Pubmed Mellott,
Divergent roles for Eph and ephrin in avian cranial neural crest.
2008,
Pubmed Milet,
Dissection of Xenopus laevis neural crest for in vitro explant culture or in vivo transplantation.
2014,
Pubmed
,
Xenbase Moody,
Cell lineage analysis in Xenopus embryos.
2000,
Pubmed
,
Xenbase Nakayama,
Simple and efficient CRISPR/Cas9-mediated targeted mutagenesis in Xenopus tropicalis.
2013,
Pubmed
,
Xenbase Nakayama,
Cas9-based genome editing in Xenopus tropicalis.
2014,
Pubmed
,
Xenbase Nandadasa,
N- and E-cadherins in Xenopus are specifically required in the neural and non-neural ectoderm, respectively, for F-actin assembly and morphogenetic movements.
2009,
Pubmed
,
Xenbase Noh,
In vivo expression of ephrinA5-Fc in mice results in cephalic neural crest agenesis and craniofacial abnormalities.
2014,
Pubmed Olesnicky Killian,
A role for chemokine signaling in neural crest cell migration and craniofacial development.
2009,
Pubmed Palacios,
Lysosomal targeting of E-cadherin: a unique mechanism for the down-regulation of cell-cell adhesion during epithelial to mesenchymal transitions.
2005,
Pubmed Santiago,
Ephrin-B ligands play a dual role in the control of neural crest cell migration.
2002,
Pubmed Sato,
FGF8 signaling is chemotactic for cardiac neural crest cells.
2011,
Pubmed Scarpa,
Cadherin Switch during EMT in Neural Crest Cells Leads to Contact Inhibition of Locomotion via Repolarization of Forces.
2015,
Pubmed
,
Xenbase Sheldahl,
Dishevelled activates Ca2+ flux, PKC, and CamKII in vertebrate embryos.
2003,
Pubmed
,
Xenbase Shnitsar,
PTK7 recruits dsh to regulate neural crest migration.
2008,
Pubmed
,
Xenbase Smith,
The EphA4 and EphB1 receptor tyrosine kinases and ephrin-B2 ligand regulate targeted migration of branchial neural crest cells.
1997,
Pubmed
,
Xenbase Stramer,
Mechanisms and in vivo functions of contact inhibition of locomotion.
2017,
Pubmed Tanaka,
Association of Dishevelled with Eph tyrosine kinase receptor and ephrin mediates cell repulsion.
2003,
Pubmed
,
Xenbase Tanaka,
Phosphorylation of ephrin-B1 via the interaction with claudin following cell-cell contact formation.
2005,
Pubmed Theveneau,
Collective chemotaxis requires contact-dependent cell polarity.
2010,
Pubmed
,
Xenbase Theveneau,
Chase-and-run between adjacent cell populations promotes directional collective migration.
2013,
Pubmed Theveneau,
Neural crest migration: interplay between chemorepellents, chemoattractants, contact inhibition, epithelial-mesenchymal transition, and collective cell migration.
2012,
Pubmed Theveneau,
Neural crest delamination and migration: from epithelium-to-mesenchyme transition to collective cell migration.
2012,
Pubmed
,
Xenbase Uytterhoeven,
Loss of skywalker reveals synaptic endosomes as sorting stations for synaptic vesicle proteins.
2011,
Pubmed Wei,
ADAM13 induces cranial neural crest by cleaving class B Ephrins and regulating Wnt signaling.
2010,
Pubmed
,
Xenbase Young,
Guidance cues involved in the development of the peripheral autonomic nervous system.
2004,
Pubmed Yu,
Semaphorin signaling guides cranial neural crest cell migration in zebrafish.
2005,
Pubmed Zerial,
Rab proteins as membrane organizers.
2001,
Pubmed Zimmer,
EphB-ephrinB bi-directional endocytosis terminates adhesion allowing contact mediated repulsion.
2003,
Pubmed
