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Chromosome segregation requires the centromere, the site on chromosomes where kinetochores assemble in mitosis to attach chromosomes to the mitotic spindle. Centromere identity is defined epigenetically by the presence of nucleosomes containing the histone H3 variant CENP-A. New CENP-A nucleosome assembly occurs at the centromere every cell cycle during G1, but how CENP-A nucleosome assembly is spatially and temporally restricted remains poorly understood. Centromere recruitment of factors required for CENP-A assembly is mediated in part by the three-protein Mis18 complex (Mis18α, Mis18β, M18BP1). Here, we show that Xenopus M18BP1 localizes to centromeres during metaphase-prior to CENP-A assembly-by binding to CENP-C using a highly conserved SANTA domain. We find that Cdk phosphorylation of M18BP1 is necessary for M18BP1 to bind CENP-C and localize to centromeres in metaphase. Surprisingly, mutations which disrupt the metaphase M18BP1/CENP-C interaction cause defective nuclear localization of M18BP1 in interphase, resulting in defective CENP-A nucleosome assembly. We propose that M18BP1 may identify centromeric sites in metaphase for subsequent CENP-A nucleosome assembly in interphase.
Barnhart,
HJURP is a CENP-A chromatin assembly factor sufficient to form a functional de novo kinetochore.
2011, Pubmed
Barnhart,
HJURP is a CENP-A chromatin assembly factor sufficient to form a functional de novo kinetochore.
2011,
Pubmed Bernad,
Xenopus HJURP and condensin II are required for CENP-A assembly.
2011,
Pubmed
,
Xenbase Blow,
A role for the nuclear envelope in controlling DNA replication within the cell cycle.
1988,
Pubmed
,
Xenbase Budde,
Regulation of Op18 during spindle assembly in Xenopus egg extracts.
2001,
Pubmed
,
Xenbase Carroll,
Dual recognition of CENP-A nucleosomes is required for centromere assembly.
2010,
Pubmed Chen,
Characterization of spindle assembly checkpoint in Xenopus egg extracts.
1997,
Pubmed
,
Xenbase Dambacher,
CENP-C facilitates the recruitment of M18BP1 to centromeric chromatin.
2012,
Pubmed Desai,
The use of Xenopus egg extracts to study mitotic spindle assembly and function in vitro.
1999,
Pubmed
,
Xenbase Dunleavy,
HJURP is a cell-cycle-dependent maintenance and deposition factor of CENP-A at centromeres.
2009,
Pubmed Fachinetti,
A two-step mechanism for epigenetic specification of centromere identity and function.
2013,
Pubmed Fachinetti,
CENP-A Modifications on Ser68 and Lys124 Are Dispensable for Establishment, Maintenance, and Long-Term Function of Human Centromeres.
2017,
Pubmed Fachinetti,
DNA Sequence-Specific Binding of CENP-B Enhances the Fidelity of Human Centromere Function.
2015,
Pubmed Foltz,
Centromere-specific assembly of CENP-a nucleosomes is mediated by HJURP.
2009,
Pubmed French,
Xenopus laevis M18BP1 Directly Binds Existing CENP-A Nucleosomes to Promote Centromeric Chromatin Assembly.
2017,
Pubmed
,
Xenbase Fujita,
Priming of centromere for CENP-A recruitment by human hMis18alpha, hMis18beta, and M18BP1.
2007,
Pubmed Gadea,
Aurora kinase inhibitor ZM447439 blocks chromosome-induced spindle assembly, the completion of chromosome condensation, and the establishment of the spindle integrity checkpoint in Xenopus egg extracts.
2005,
Pubmed
,
Xenbase Guse,
A cell-free system for functional centromere and kinetochore assembly.
2012,
Pubmed
,
Xenbase Guse,
In vitro centromere and kinetochore assembly on defined chromatin templates.
2011,
Pubmed
,
Xenbase Hayashi,
Mis16 and Mis18 are required for CENP-A loading and histone deacetylation at centromeres.
2004,
Pubmed Holland,
Inducible, reversible system for the rapid and complete degradation of proteins in mammalian cells.
2012,
Pubmed Hori,
Association of M18BP1/KNL2 with CENP-A Nucleosome Is Essential for Centromere Formation in Non-mammalian Vertebrates.
2017,
Pubmed Hu,
Structure of a CENP-A-histone H4 heterodimer in complex with chaperone HJURP.
2011,
Pubmed Jansen,
Propagation of centromeric chromatin requires exit from mitosis.
2007,
Pubmed Karpen,
The case for epigenetic effects on centromere identity and function.
1997,
Pubmed Kato,
A conserved mechanism for centromeric nucleosome recognition by centromere protein CENP-C.
2013,
Pubmed Katoh,
MAFFT: a novel method for rapid multiple sequence alignment based on fast Fourier transform.
2002,
Pubmed Kim,
Roles of Mis18α in epigenetic regulation of centromeric chromatin and CENP-A loading.
2012,
Pubmed Kral,
Possible identification of CENP-C in fish and the presence of the CENP-C motif in M18BP1 of vertebrates.
2015,
Pubmed Lagana,
A small GTPase molecular switch regulates epigenetic centromere maintenance by stabilizing newly incorporated CENP-A.
2010,
Pubmed Lermontova,
Arabidopsis kinetochore null2 is an upstream component for centromeric histone H3 variant cenH3 deposition at centromeres.
2013,
Pubmed Lowary,
New DNA sequence rules for high affinity binding to histone octamer and sequence-directed nucleosome positioning.
1998,
Pubmed Maddox,
Functional genomics identifies a Myb domain-containing protein family required for assembly of CENP-A chromatin.
2007,
Pubmed McKinley,
The molecular basis for centromere identity and function.
2016,
Pubmed McKinley,
Polo-like kinase 1 licenses CENP-A deposition at centromeres.
2014,
Pubmed Mendiburo,
Drosophila CENH3 is sufficient for centromere formation.
2011,
Pubmed Milks,
Dissection of CENP-C-directed centromere and kinetochore assembly.
2009,
Pubmed
,
Xenbase Moree,
CENP-C recruits M18BP1 to centromeres to promote CENP-A chromatin assembly.
2011,
Pubmed
,
Xenbase Müller,
Phosphorylation and DNA binding of HJURP determine its centromeric recruitment and function in CenH3(CENP-A) loading.
2014,
Pubmed Musacchio,
A Molecular View of Kinetochore Assembly and Function.
2017,
Pubmed Nardi,
Licensing of Centromeric Chromatin Assembly through the Mis18α-Mis18β Heterotetramer.
2016,
Pubmed Nishimura,
An auxin-based degron system for the rapid depletion of proteins in nonplant cells.
2009,
Pubmed Ohzeki,
Breaking the HAC Barrier: histone H3K9 acetyl/methyl balance regulates CENP-A assembly.
2012,
Pubmed Ohzeki,
KAT7/HBO1/MYST2 Regulates CENP-A Chromatin Assembly by Antagonizing Suv39h1-Mediated Centromere Inactivation.
2016,
Pubmed Palmer,
A 17-kD centromere protein (CENP-A) copurifies with nucleosome core particles and with histones.
1987,
Pubmed Pan,
CDK-regulated dimerization of M18BP1 on a Mis18 hexamer is necessary for CENP-A loading.
2017,
Pubmed Perpelescu,
HJURP is involved in the expansion of centromeric chromatin.
2015,
Pubmed Ran,
Genome engineering using the CRISPR-Cas9 system.
2013,
Pubmed Sandmann,
Targeting of Arabidopsis KNL2 to Centromeres Depends on the Conserved CENPC-k Motif in Its C Terminus.
2017,
Pubmed Santaguida,
Dissecting the role of MPS1 in chromosome biorientation and the spindle checkpoint through the small molecule inhibitor reversine.
2010,
Pubmed Session,
Genome evolution in the allotetraploid frog Xenopus laevis.
2016,
Pubmed
,
Xenbase Shang,
Acetylation of histone H4 lysine 5 and 12 is required for CENP-A deposition into centromeres.
2016,
Pubmed Shono,
CENP-C and CENP-I are key connecting factors for kinetochore and CENP-A assembly.
2015,
Pubmed Silva,
Cdk activity couples epigenetic centromere inheritance to cell cycle progression.
2012,
Pubmed Spiller,
Molecular basis for Cdk1-regulated timing of Mis18 complex assembly and CENP-A deposition.
2017,
Pubmed Stankovic,
A Dual Inhibitory Mechanism Sufficient to Maintain Cell-Cycle-Restricted CENP-A Assembly.
2017,
Pubmed Stellfox,
Differential Binding Partners of the Mis18α/β YIPPEE Domains Regulate Mis18 Complex Recruitment to Centromeres.
2016,
Pubmed Stoler,
A mutation in CSE4, an essential gene encoding a novel chromatin-associated protein in yeast, causes chromosome nondisjunction and cell cycle arrest at mitosis.
1995,
Pubmed Tachiwana,
HJURP involvement in de novo CenH3(CENP-A) and CENP-C recruitment.
2015,
Pubmed Tan,
A modular polycistronic expression system for overexpressing protein complexes in Escherichia coli.
2001,
Pubmed Van Hooser,
Specification of kinetochore-forming chromatin by the histone H3 variant CENP-A.
2001,
Pubmed Wang,
Mitotic regulator Mis18β interacts with and specifies the centromeric assembly of molecular chaperone holliday junction recognition protein (HJURP).
2014,
Pubmed Westhorpe,
The centromere: epigenetic control of chromosome segregation during mitosis.
2014,
Pubmed Westhorpe,
A cell-free CENP-A assembly system defines the chromatin requirements for centromere maintenance.
2015,
Pubmed
,
Xenbase Yoda,
Human centromere protein A (CENP-A) can replace histone H3 in nucleosome reconstitution in vitro.
2000,
Pubmed Yu,
Dynamic phosphorylation of CENP-A at Ser68 orchestrates its cell-cycle-dependent deposition at centromeres.
2015,
Pubmed Zhang,
SANTA domain: a novel conserved protein module in Eukaryota with potential involvement in chromatin regulation.
2006,
Pubmed
