Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
J Membr Biol
2005 Oct 01;2073:169-81. doi: 10.1007/s00232-005-0812-1.
Show Gene links
Show Anatomy links
Interactions between charged residues in the transmembrane segments of the voltage-sensing domain in the hERG channel.
Zhang M, Liu J, Jiang M, Wu DM, Sonawane K, Guy HR, Tseng GN.
???displayArticle.abstract???
Studies on voltage-gated K channels such as Shaker have shown that positive charges in the voltage-sensor (S4) can form salt bridges with negative charges in the surrounding transmembrane segments in a state-dependent manner, and different charge pairings can stabilize the channels in closed or open states. The goal of this study is to identify such charge interactions in the hERG channel. This knowledge can provide constraints on the spatial relationship among transmembrane segments in the channel's voltage-sensing domain, which are necessary for modeling its structure. We first study the effects of reversing S4's positive charges on channel activation. Reversing positive charges at the outer (K525D) and inner (K538D) ends of S4 markedly accelerates hERG activation, whereas reversing the 4 positive charges in between either has no effect or slows activation. We then use the 'mutant cycle analysis' to test whether D456 (outer end of S2) and D411 (inner end of S1) can pair with K525 and K538, respectively. Other positive charges predicted to be able, or unable, to interact with D456 or D411 are also included in the analysis. The results are consistent with predictions based on the distribution of these charged residues, and confirm that there is functional coupling between D456 and K525 and between D411 and K538.
Ahern,
Specificity of charge-carrying residues in the voltage sensor of potassium channels.
2004, Pubmed
Ahern,
Specificity of charge-carrying residues in the voltage sensor of potassium channels.
2004,
Pubmed Bauer,
HERG K(+) currents in human prolactin-secreting adenoma cells.
2003,
Pubmed Broomand,
Molecular movement of the voltage sensor in a K channel.
2003,
Pubmed
,
Xenbase Cohen,
Answers and questions from the KvAP structures.
2003,
Pubmed Doyle,
The structure of the potassium channel: molecular basis of K+ conduction and selectivity.
1998,
Pubmed Faravelli,
A HERG-like K+ channel in rat F-11 DRG cell line: pharmacological identification and biophysical characterization.
1996,
Pubmed Gandhi,
The orientation and molecular movement of a k(+) channel voltage-sensing domain.
2003,
Pubmed
,
Xenbase Gonzalez,
Periodic perturbations in Shaker K+ channel gating kinetics by deletions in the S3-S4 linker.
2001,
Pubmed
,
Xenbase Hidalgo,
Revealing the architecture of a K+ channel pore through mutant cycles with a peptide inhibitor.
1995,
Pubmed
,
Xenbase Horn,
How S4 segments move charge. Let me count the ways.
2004,
Pubmed Jiang,
The principle of gating charge movement in a voltage-dependent K+ channel.
2003,
Pubmed Jiang,
X-ray structure of a voltage-dependent K+ channel.
2003,
Pubmed Jiang,
The open pore conformation of potassium channels.
2002,
Pubmed Kuo,
Crystal structure of the potassium channel KirBac1.1 in the closed state.
2003,
Pubmed Lainé,
Atomic proximity between S4 segment and pore domain in Shaker potassium channels.
2003,
Pubmed
,
Xenbase Lee,
Interaction between extracellular Hanatoxin and the resting conformation of the voltage-sensor paddle in Kv channels.
2003,
Pubmed
,
Xenbase Li-Smerin,
alpha-helical structural elements within the voltage-sensing domains of a K(+) channel.
2000,
Pubmed
,
Xenbase Liu,
Negative charges in the transmembrane domains of the HERG K channel are involved in the activation- and deactivation-gating processes.
2003,
Pubmed
,
Xenbase Long,
Crystal structure of a mammalian voltage-dependent Shaker family K+ channel.
2005,
Pubmed Long,
Voltage sensor of Kv1.2: structural basis of electromechanical coupling.
2005,
Pubmed Lu,
Effects of premature stimulation on HERG K(+) channels.
2001,
Pubmed Mitcheson,
Trapping of a methanesulfonanilide by closure of the HERG potassium channel activation gate.
2000,
Pubmed
,
Xenbase Papazian,
Structural organization of the voltage sensor in voltage-dependent potassium channels.
2002,
Pubmed Piper,
Gating currents associated with intramembrane charge displacement in HERG potassium channels.
2003,
Pubmed
,
Xenbase Roden,
Drug-induced prolongation of the QT interval.
2004,
Pubmed Rosati,
Glucose- and arginine-induced insulin secretion by human pancreatic beta-cells: the role of HERG K(+) channels in firing and release.
2000,
Pubmed Sanguinetti,
A mechanistic link between an inherited and an acquired cardiac arrhythmia: HERG encodes the IKr potassium channel.
1995,
Pubmed
,
Xenbase Schreibmayer,
Voltage clamping of Xenopus laevis oocytes utilizing agarose-cushion electrodes.
1994,
Pubmed
,
Xenbase Shoeb,
Cloning and functional characterization of the smooth muscle ether-a-go-go-related gene K+ channel. Potential role of a conserved amino acid substitution in the S4 region.
2003,
Pubmed
,
Xenbase Silverman,
Structural basis of two-stage voltage-dependent activation in K+ channels.
2003,
Pubmed
,
Xenbase Smith,
Fast and slow voltage sensor movements in HERG potassium channels.
2002,
Pubmed
,
Xenbase Starace,
A proton pore in a potassium channel voltage sensor reveals a focused electric field.
2004,
Pubmed Tiwari-Woodruff,
Voltage-dependent structural interactions in the Shaker K(+) channel.
2000,
Pubmed
,
Xenbase Tseng-Crank,
Molecular cloning and functional expression of a potassium channel cDNA isolated from a rat cardiac library.
1990,
Pubmed
,
Xenbase Wang,
A quantitative analysis of the activation and inactivation kinetics of HERG expressed in Xenopus oocytes.
1997,
Pubmed
,
Xenbase Wang,
The seizure locus encodes the Drosophila homolog of the HERG potassium channel.
1997,
Pubmed Yifrach,
Energetics of pore opening in a voltage-gated K(+) channel.
2002,
Pubmed
,
Xenbase Zhang,
Gating charges in the activation and inactivation processes of the HERG channel.
2004,
Pubmed
,
Xenbase Zhou,
HERG-like K+ channels in microglia.
1998,
Pubmed
