Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Mol Biol Cell
2003 Mar 01;143:1125-37. doi: 10.1091/mbc.e02-08-0511.
Show Gene links
Show Anatomy links
Pre-M phase-promoting factor associates with annulate lamellae in Xenopus oocytes and egg extracts.
Beckhelling C, Chang P, Chevalier S, Ford C, Houliston E.
???displayArticle.abstract???
We have used complementary biochemical and in vivo approaches to study the compartmentalization of M phase-promoting factor (MPF) in prophase Xenopus eggs and oocytes. We first examined the distribution of MPF (Cdc2/CyclinB2) and membranous organelles in high-speed extracts of Xenopus eggs made during mitotic prophase. These extracts were found to lack mitochondria, Golgi membranes, and most endoplasmic reticulum (ER) but to contain the bulk of the pre-MPF pool. This pre-MPF could be pelleted by further centrifugation along with components necessary to activate it. On activation, Cdc2/CyclinB2 moved into the soluble fraction. Electron microscopy and Western blot analysis showed that the pre-MPF pellet contained a specific ER subdomain comprising "annulate lamellae" (AL): stacked ER membranes highly enriched in nuclear pores. Colocalization of pre-MPF with AL was demonstrated by anti-CyclinB2 immunofluorescence in prophase oocytes, in which AL are positioned close to the vegetal surface. Green fluorescent protein-CyclinB2 expressed in oocytes also localized at AL. These data suggest that inactive MPF associates with nuclear envelope components just before activation. This association may explain why nuclei and centrosomes stimulate MPF activation and provide a mechanism for targeting of MPF to some of its key substrates.
Alfa,
Distinct nuclear and spindle pole body population of cyclin-cdc2 in fission yeast.
1990, Pubmed
Alfa,
Distinct nuclear and spindle pole body population of cyclin-cdc2 in fission yeast.
1990,
Pubmed Argon,
GRP94, an ER chaperone with protein and peptide binding properties.
1999,
Pubmed Ashcroft,
RNA-Mediated interference of a cdc25 homolog in Caenorhabditis elegans results in defects in the embryonic cortical membrane, meiosis, and mitosis.
1999,
Pubmed Audit,
Cyclin B (p56cdc13) localization in the yeast Schizosaccharomyces pombe: an ultrastructural and immunocytochemical study.
1996,
Pubmed Bagley,
The nuclear pore complex.
2000,
Pubmed Bailly,
p34cdc2 is located in both nucleus and cytoplasm; part is centrosomally associated at G2/M and enters vesicles at anaphase.
1989,
Pubmed Bailly,
Cytoplasmic accumulation of cyclin B1 in human cells: association with a detergent-resistant compartment and with the centrosome.
1992,
Pubmed Bal,
Origin and fate of annulate lamellae in Arbacia punctulata eggs.
1968,
Pubmed Beaudouin,
Nuclear envelope breakdown proceeds by microtubule-induced tearing of the lamina.
2002,
Pubmed Beckhelling,
Timing of calcium and protein synthesis requirements for the first mitotic cell cycle in fertilised Xenopus eggs.
1999,
Pubmed
,
Xenbase Beckhelling,
Maturation promoting factor activation in early amphibian embryos: temporal and spatial control.
1998,
Pubmed Beckhelling,
Localised MPF regulation in eggs.
2000,
Pubmed
,
Xenbase Borgne,
Intra-M phase-promoting factor phosphorylation of cyclin B at the prophase/metaphase transition.
1999,
Pubmed
,
Xenbase Brunati,
GRP94 (endoplasmin) co-purifies with and is phosphorylated by Golgi apparatus casein kinase.
2000,
Pubmed Charrasse,
The Xenopus XMAP215 and its human homologue TOG proteins interact with cyclin B1 to target p34cdc2 to microtubules during mitosis.
2000,
Pubmed
,
Xenbase Clute,
Temporal and spatial control of cyclin B1 destruction in metaphase.
1999,
Pubmed Cordes,
Cytoplasmic annulate lamellae in cultured cells: composition, distribution, and mitotic behavior.
1996,
Pubmed Cyert,
Regulation of MPF activity in vitro.
1988,
Pubmed
,
Xenbase Dabauvalle,
Spontaneous assembly of pore complex-containing membranes ("annulate lamellae") in Xenopus egg extract in the absence of chromatin.
1991,
Pubmed
,
Xenbase De Souza,
Centrosomal and cytoplasmic Cdc2/cyclin B1 activation precedes nuclear mitotic events.
2000,
Pubmed DETTLAFF,
THE R OLE OF THE GERMINAL VESICLE IN OOECYTE MATURATION IN ANURANS AS REVEALED BY THE REMOVAL AND TRANSPLANTATION OF NUCLEI.
1964,
Pubmed Dumont,
Oogenesis in Xenopus laevis (Daudin). I. Stages of oocyte development in laboratory maintained animals.
1972,
Pubmed
,
Xenbase Edgar,
Distinct molecular mechanism regulate cell cycle timing at successive stages of Drosophila embryogenesis.
1994,
Pubmed Felix,
A post-ribosomal supernatant from activated Xenopus eggs that displays post-translationally regulated oscillation of its cdc2+ mitotic kinase activity.
1989,
Pubmed
,
Xenbase Gerhart,
Cell cycle dynamics of an M-phase-specific cytoplasmic factor in Xenopus laevis oocytes and eggs.
1984,
Pubmed
,
Xenbase Hagting,
Translocation of cyclin B1 to the nucleus at prophase requires a phosphorylation-dependent nuclear import signal.
1999,
Pubmed Hang,
Association of the human SUMO-1 protease SENP2 with the nuclear pore.
2002,
Pubmed
,
Xenbase Hara,
A cytoplasmic clock with the same period as the division cycle in Xenopus eggs.
1980,
Pubmed
,
Xenbase Hartley,
In vivo regulation of the early embryonic cell cycle in Xenopus.
1996,
Pubmed
,
Xenbase Heim,
Improved green fluorescence.
1995,
Pubmed Imreh,
An integral membrane protein from the nuclear pore complex is also present in the annulate lamellae: implications for annulate lamella formation.
2000,
Pubmed Iwao,
The egg nucleus regulates the behavior of sperm nuclei as well as cycling of MPF in physiologically polyspermic newt eggs.
1993,
Pubmed
,
Xenbase Iwao,
Control of sperm nuclear behavior in physiologically polyspermic newt eggs: possible involvement of MPF.
1990,
Pubmed
,
Xenbase Iwashita,
Essential role of germinal vesicle material in the meiotic cell cycle of Xenopus oocytes.
1998,
Pubmed
,
Xenbase Jackman,
Human cyclins B1 and B2 are localized to strikingly different structures: B1 to microtubules, B2 primarily to the Golgi apparatus.
1995,
Pubmed Kallenbach,
'De novo' centrioles originate at sites associated with annulate lamellae in sea-urchin eggs.
1982,
Pubmed Kessel,
Annulate lamellae: a last frontier in cellular organelles.
1992,
Pubmed Kimelman,
The events of the midblastula transition in Xenopus are regulated by changes in the cell cycle.
1987,
Pubmed
,
Xenbase Kobayashi,
On the synthesis and destruction of A- and B-type cyclins during oogenesis and meiotic maturation in Xenopus laevis.
1991,
Pubmed
,
Xenbase Laemmli,
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
1970,
Pubmed Leiss,
Association of cyclin-bound p34cdc2 with subcellular structures in xenopus eggs.
1992,
Pubmed
,
Xenbase Li,
Nuclear localization of cyclin B1 mediates its biological activity and is regulated by phosphorylation.
1997,
Pubmed
,
Xenbase Lindsay,
Calcium requirements during mitotic cdc2 kinase activation and cyclin degradation in Xenopus egg extracts.
1995,
Pubmed
,
Xenbase Liu,
The human Myt1 kinase preferentially phosphorylates Cdc2 on threonine 14 and localizes to the endoplasmic reticulum and Golgi complex.
1997,
Pubmed
,
Xenbase Masui,
Cytoplasmic control of nuclear behavior during meiotic maturation of frog oocytes.
1971,
Pubmed Meier,
Nuclear pore complex assembly studied with a biochemical assay for annulate lamellae formation.
1995,
Pubmed
,
Xenbase Moreno,
Substrates for p34cdc2: in vivo veritas?
1990,
Pubmed Morgan,
Regulation of the APC and the exit from mitosis.
1999,
Pubmed Morgan,
Cyclin-dependent kinases: engines, clocks, and microprocessors.
1997,
Pubmed Murray,
Cyclin synthesis drives the early embryonic cell cycle.
1989,
Pubmed
,
Xenbase Murray,
The role of cyclin synthesis and degradation in the control of maturation promoting factor activity.
1989,
Pubmed
,
Xenbase Newport,
Regulation of the cell cycle during early Xenopus development.
1984,
Pubmed
,
Xenbase Nigg,
Cyclin-dependent protein kinases: key regulators of the eukaryotic cell cycle.
1995,
Pubmed Norbury,
Animal cell cycles and their control.
1992,
Pubmed Novak,
Numerical analysis of a comprehensive model of M-phase control in Xenopus oocyte extracts and intact embryos.
1993,
Pubmed
,
Xenbase Nurse,
Universal control mechanism regulating onset of M-phase.
1990,
Pubmed O'Farrell,
Triggering the all-or-nothing switch into mitosis.
2001,
Pubmed Ohi,
Regulating the onset of mitosis.
1999,
Pubmed
,
Xenbase Ookata,
Relocation and distinct subcellular localization of p34cdc2-cyclin B complex at meiosis reinitiation in starfish oocytes.
1992,
Pubmed Panté,
Interactions and three-dimensional localization of a group of nuclear pore complex proteins.
1994,
Pubmed
,
Xenbase Pérez-Mongiovi,
A propagated wave of MPF activation accompanies surface contraction waves at first mitosis in Xenopus.
1998,
Pubmed
,
Xenbase Pérez-Mongiovi,
Nuclei and microtubule asters stimulate maturation/M phase promoting factor (MPF) activation in Xenopus eggs and egg cytoplasmic extracts.
2000,
Pubmed
,
Xenbase Pines,
Four-dimensional control of the cell cycle.
1999,
Pubmed Pitt,
P granules in the germ cells of Caenorhabditis elegans adults are associated with clusters of nuclear pores and contain RNA.
2000,
Pubmed Pockwinse,
Cell cycle independent interaction of CDC2 with the centrosome, which is associated with the nuclear matrix-intermediate filament scaffold.
1997,
Pubmed Raff,
Two distinct mechanisms localise cyclin B transcripts in syncytial Drosophila embryos.
1990,
Pubmed Rankin,
The surface contraction waves of Xenopus eggs reflect the metachronous cell-cycle state of the cytoplasm.
1997,
Pubmed
,
Xenbase Reinsch,
Movement of nuclei along microtubules in Xenopus egg extracts.
1997,
Pubmed
,
Xenbase Rieder,
Intranuclear membranes and the formation of the first meiotic spindle in Xenos peckii (Acroschismus wheeleri) oocytes.
1983,
Pubmed Roegiers,
Phases of cytoplasmic and cortical reorganizations of the ascidian zygote between fertilization and first division.
1999,
Pubmed Sakamoto,
Changes in cyclin B during oocyte maturation and early embryonic cell cycle in the newt, Cynops pyrrhogaster: requirement of germinal vesicle for MPF activation.
1998,
Pubmed Salina,
Cytoplasmic dynein as a facilitator of nuclear envelope breakdown.
2002,
Pubmed Shinagawa,
Nuclear Involvement in Localization of the Initiation Site of Surface Contraction Waves in Xenopus Eggs: (surface contraction waves/Xenopus laevis/nucleus/colchicine/cleavage).
1989,
Pubmed
,
Xenbase Shinagawa,
The interval of the cytoplasmic cycle observed in non-nucleate egg fragments is longer than that of the cleavage cycle in normal eggs of Xenopus laevis.
1983,
Pubmed
,
Xenbase Shinagawa,
Relative Timing of Stiffening with Various Combinations of Nucleate and Enucleated Egg Fragments of Xenopus laevis: (egg stiffening/Xenopus laevis/cell cycle/timing system).
1992,
Pubmed
,
Xenbase Solomon,
Cyclin activation of p34cdc2.
1990,
Pubmed
,
Xenbase Takahashi,
Yeast Ulp1, an Smt3-specific protease, associates with nucleoporins.
2000,
Pubmed Takizawa,
Control of mitosis by changes in the subcellular location of cyclin-B1-Cdk1 and Cdc25C.
2000,
Pubmed
,
Xenbase Terasaki,
Changes in organization of the endoplasmic reticulum during Xenopus oocyte maturation and activation.
2001,
Pubmed
,
Xenbase Wakefield,
Centrosomes have a role in regulating the destruction of cyclin B in early Drosophila embryos.
2000,
Pubmed Xu,
Mouse VDAC isoforms expressed in yeast: channel properties and their roles in mitochondrial outer membrane permeability.
1999,
Pubmed Yang,
Control of cyclin B1 localization through regulated binding of the nuclear export factor CRM1.
1998,
Pubmed
,
Xenbase Zernicka-Goetz,
An indelible lineage marker for Xenopus using a mutated green fluorescent protein.
1996,
Pubmed
,
Xenbase
