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Proc Natl Acad Sci U S A
2006 Mar 28;10313:5149-54. doi: 10.1073/pnas.0600847103.
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The cholinergic antagonist alpha-bungarotoxin also binds and blocks a subset of GABA receptors.
McCann CM, Bracamontes J, Steinbach JH, Sanes JR.
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The polypeptide snake toxin alpha-bungarotoxin (BTX) has been used in hundreds of studies on the structure, function, and development of the neuromuscular junction because it binds tightly and specifically to the nicotinic acetylcholine receptors (nAChRs) at this synapse. We show here that BTX also binds to and blocks a subset of GABA(A) receptors (GABA(A)Rs) that contain the GABA(A)R beta3 subunit. These results introduce a previously unrecognized tool for analysis of GABA(A)Rs but may complicate interpretation of some studies on neuronal nAChRs.
Anderson,
Nerve-induced and spontaneous redistribution of acetylcholine receptors on cultured muscle cells.
1977, Pubmed,
Xenbase
Anderson,
Nerve-induced and spontaneous redistribution of acetylcholine receptors on cultured muscle cells.
1977,
Pubmed
,
Xenbase Balass,
The alpha-bungarotoxin binding site on the nicotinic acetylcholine receptor: analysis using a phage-epitope library.
1997,
Pubmed Baumann,
Subunit arrangement of gamma-aminobutyric acid type A receptors.
2001,
Pubmed
,
Xenbase Baumann,
Forced subunit assembly in alpha1beta2gamma2 GABAA receptors. Insight into the absolute arrangement.
2002,
Pubmed
,
Xenbase Biwersi,
Cell-permeable fluorescent indicator for cytosolic chloride.
1991,
Pubmed Chang,
Stoichiometry of a recombinant GABAA receptor.
1996,
Pubmed
,
Xenbase Changeux,
Studies on the cholinergic receptor protein of Electrophorus electricus. I. An assay in vitro for the cholinergic receptor site and solubilization of the receptor protein from electric tissue.
1971,
Pubmed Chen,
The alpha-bungarotoxin-binding nicotinic acetylcholine receptor from rat brain contains only the alpha7 subunit.
1997,
Pubmed
,
Xenbase Colquhoun,
Pharmacology of neuronal nicotinic acetylcholine receptor subtypes.
1997,
Pubmed Connolly,
Subcellular localization of gamma-aminobutyric acid type A receptors is determined by receptor beta subunits.
1996,
Pubmed Fabian-Fine,
Ultrastructural distribution of the alpha7 nicotinic acetylcholine receptor subunit in rat hippocampus.
2001,
Pubmed Fambrough,
Control of acetylcholine receptors in skeletal muscle.
1979,
Pubmed Fertuck,
Quantitation of junctional and extrajunctional acetylcholine receptors by electron microscope autoradiography after 125I-alpha-bungarotoxin binding at mouse neuromuscular junctions.
1976,
Pubmed Fuchs,
The binding site of acetylcholine receptor: from synthetic peptides to solution and crystal structure.
2003,
Pubmed Gotti,
Neuronal nicotinic receptors: from structure to pathology.
2004,
Pubmed Kawai,
Nicotinic alpha 7 receptor clusters on hippocampal GABAergic neurons: regulation by synaptic activity and neurotrophins.
2002,
Pubmed Kummer,
Assembly of the postsynaptic membrane at the neuromuscular junction: paradigm lost.
2006,
Pubmed Lee,
Influence of denervation on localization of neurotoxins from clapid venoms in rat diaphragm.
1967,
Pubmed Levandoski,
Chimeric analysis of a neuronal nicotinic acetylcholine receptor reveals amino acids conferring sensitivity to alpha-bungarotoxin.
1999,
Pubmed
,
Xenbase Marinou,
Identification of regions involved in the binding of alpha-bungarotoxin to the human alpha7 neuronal nicotinic acetylcholine receptor using synthetic peptides.
2003,
Pubmed McCann,
Peptide tags for labeling membrane proteins in live cells with multiple fluorophores.
2005,
Pubmed Merlie,
Acetylcholine receptor subunits transit a precursor pool before acquiring alpha-bungarotoxin binding activity.
1981,
Pubmed Miko,
A TM2 residue in the beta1 subunit determines spontaneous opening of homomeric and heteromeric gamma-aminobutyric acid-gated ion channels.
2004,
Pubmed
,
Xenbase Orr-Urtreger,
Mice deficient in the alpha7 neuronal nicotinic acetylcholine receptor lack alpha-bungarotoxin binding sites and hippocampal fast nicotinic currents.
1997,
Pubmed Sanes,
Development of the vertebrate neuromuscular junction.
1999,
Pubmed Sieghart,
Subunit composition, distribution and function of GABA(A) receptor subtypes.
2002,
Pubmed Tretter,
Stoichiometry and assembly of a recombinant GABAA receptor subtype.
1997,
Pubmed Wooltorton,
Pharmacological and physiological characterization of murine homomeric beta3 GABA(A) receptors.
1997,
Pubmed
,
Xenbase
