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The general field of quantitative biology has advanced significantly on the back of recent improvements in both sequencing technology and proteomics methods. The development of high-throughput, short-read sequencing has revolutionized RNA-based expression studies, while improvements in proteomics methods have enabled quantitative studies to attain better resolution. Here we introduce methods to undertake global analyses of gene expression through RNA and protein quantification in Xenopus embryos and tissues.
Amin,
Proteomic profiling of cardiac tissue by isolation of nuclei tagged in specific cell types (INTACT).
2014, Pubmed,
Xenbase
Amin,
Proteomic profiling of cardiac tissue by isolation of nuclei tagged in specific cell types (INTACT).
2014,
Pubmed
,
Xenbase Blitz,
A catalog of Xenopus tropicalis transcription factors and their regional expression in the early gastrula stage embryo.
2017,
Pubmed
,
Xenbase Briggs,
The dynamics of gene expression in vertebrate embryogenesis at single-cell resolution.
2018,
Pubmed
,
Xenbase Butler,
A novel role for sox7 in Xenopus early primordial germ cell development: mining the PGC transcriptome.
2018,
Pubmed
,
Xenbase Charney,
Foxh1 Occupies cis-Regulatory Modules Prior to Dynamic Transcription Factor Interactions Controlling the Mesendoderm Gene Program.
2017,
Pubmed
,
Xenbase Collart,
High-resolution analysis of gene activity during the Xenopus mid-blastula transition.
2014,
Pubmed
,
Xenbase De Domenico,
Molecular asymmetry in the 8-cell stage Xenopus tropicalis embryo described by single blastomere transcript sequencing.
2015,
Pubmed
,
Xenbase Ding,
Genome-wide analysis of dorsal and ventral transcriptomes of the Xenopus laevis gastrula.
2017,
Pubmed
,
Xenbase Eng,
Transcriptome-scale super-resolved imaging in tissues by RNA seqFISH.
2019,
Pubmed Klein,
Droplet barcoding for single-cell transcriptomics applied to embryonic stem cells.
2015,
Pubmed
,
Xenbase Laver,
Assessing the performance of the Oxford Nanopore Technologies MinION.
2015,
Pubmed Lindeboom,
Mass Spectrometry-Based Absolute Quantification of Single Xenopus Embryo Proteomes.
2019,
Pubmed
,
Xenbase Lombard-Banek,
Label-free Quantification of Proteins in Single Embryonic Cells with Neural Fate in the Cleavage-Stage Frog (Xenopus laevis) Embryo using Capillary Electrophoresis Electrospray Ionization High-Resolution Mass Spectrometry (CE-ESI-HRMS).
2016,
Pubmed
,
Xenbase McCarthy,
Third generation DNA sequencing: pacific biosciences' single molecule real time technology.
2010,
Pubmed Onjiko,
Single-cell mass spectrometry reveals small molecules that affect cell fates in the 16-cell embryo.
2015,
Pubmed
,
Xenbase Owens,
Measuring Absolute RNA Copy Numbers at High Temporal Resolution Reveals Transcriptome Kinetics in Development.
2016,
Pubmed
,
Xenbase Owens,
An RNA-Seq Protocol for Differential Expression Analysis.
2019,
Pubmed
,
Xenbase Palii,
Single-Cell Proteomics Reveal that Quantitative Changes in Co-expressed Lineage-Specific Transcription Factors Determine Cell Fate.
2019,
Pubmed Paraiso,
Endodermal Maternal Transcription Factors Establish Super-Enhancers during Zygotic Genome Activation.
2019,
Pubmed
,
Xenbase Peshkin,
On the Relationship of Protein and mRNA Dynamics in Vertebrate Embryonic Development.
2015,
Pubmed
,
Xenbase Presler,
Proteomics of phosphorylation and protein dynamics during fertilization and meiotic exit in the Xenopus egg.
2017,
Pubmed
,
Xenbase Smits,
Global absolute quantification reveals tight regulation of protein expression in single Xenopus eggs.
2014,
Pubmed
,
Xenbase Wasson,
INTACT Proteomics in Xenopus.
2019,
Pubmed
,
Xenbase Wühr,
Deep proteomics of the Xenopus laevis egg using an mRNA-derived reference database.
2014,
Pubmed
,
Xenbase
