Stewart M, Donahue K, Wilke E, Shifley E.

P20 GM103436 NIGMS NIH HHS

	Figure 1. (A) LOC496300 is expressed in the endoderm of Xenopus laevis from NF stage 10 through 37. It becomes restricted to the midgut and hindgut during tailbud stages (~NF stg 30) and is expressed in the intestine and gall bladder of NF stage 42-43 gut tubes at differing levels (i.e. gut tube a and b). Embryos hybridized with the sense probe for LOC496300 show some non-specific staining in the head at tailbud stages, but overall no specific staining in the endoderm. NF stage 43 organ buds are marked as follows: lv, liver; p, pancreas; s, stomach; gb, gall bladder; i, intestine. (B) NF stage 10, 12, 28 and 35 embryos were sectioned to show the punctate expression pattern of LOC496300 throughout the early endoderm and in the dorsal and ventral tailbud endoderm. The numbered lines mark the approximate level of each section of the tailbud embryos and the dotted red line outlines the endoderm.
	XB5947952 (hypothetical LOC496300) gene expression in bissected X. laevis embryo, NF stage 10, dorsal right.
	XB5947952 (hypothetical LOC496300) gene expression in X. laevis embryo, NF stage 24, lateral view, dorsal up, anterior left.
	XB5947952 (hypothetical LOC496300) gene expression in isolated gut tubes of X. laevis embryo, NF stage 43, anterior left. NF stage 43 Key to developing foregut organs: lv, liver; p, pancreas; s, stomach; gb, gall bladder; i, intestine.

Chalmers, Development of the gut in Xenopus laevis. 1998, Pubmed, Xenbase

Chalmers, Development of the gut in Xenopus laevis. 1998, Pubmed , Xenbase
Chalmers, The Xenopus tadpole gut: fate maps and morphogenetic movements. 2000, Pubmed , Xenbase
Chen, Cell-autonomous and signal-dependent expression of liver and intestine marker genes in pluripotent precursor cells from Xenopus embryos. 2003, Pubmed , Xenbase
Costa, Novel gene expression domains reveal early patterning of the Xenopus endoderm. 2003, Pubmed , Xenbase
Hudson, Xsox17alpha and -beta mediate endoderm formation in Xenopus. 1997, Pubmed , Xenbase
Ishizuya-Oka, Amphibian organ remodeling during metamorphosis: insight into thyroid hormone-induced apoptosis. 2011, Pubmed , Xenbase
Ishizuya-Oka, Apoptosis of larval cells during amphibian metamorphosis. 1996, Pubmed , Xenbase
Ishizuya-Oka, Origin of the adult intestinal stem cells induced by thyroid hormone in Xenopus laevis. 2009, Pubmed , Xenbase
Marshall, Cell specialization in the epithelium of the small intestine of feeding Xenopus laevis tadpoles. 1978, Pubmed , Xenbase
Park, Xenopus cDNA microarray identification of genes with endodermal organ expression. 2007, Pubmed , Xenbase
Pera, Darmin is a novel secreted protein expressed during endoderm development in Xenopus. 2003, Pubmed , Xenbase
Rankin, Timing is everything: Reiterative Wnt, BMP and RA signaling regulate developmental competence during endoderm organogenesis. 2018, Pubmed , Xenbase
Shi, Biphasic intestinal development in amphibians: embryogenesis and remodeling during metamorphosis. 1996, Pubmed , Xenbase
Sinner, Sox17 and beta-catenin cooperate to regulate the transcription of endodermal genes. 2004, Pubmed , Xenbase
Stevens, Genomic integration of Wnt/β-catenin and BMP/Smad1 signaling coordinates foregut and hindgut transcriptional programs. 2017, Pubmed , Xenbase
Sun, Expression profiling of intestinal tissues implicates tissue-specific genes and pathways essential for thyroid hormone-induced adult stem cell development. 2013, Pubmed , Xenbase
Womble, Frogs as integrative models for understanding digestive organ development and evolution. 2016, Pubmed , Xenbase
Zorn, Gene expression in the embryonic Xenopus liver. 2001, Pubmed , Xenbase
Zorn, Vertebrate endoderm development and organ formation. 2009, Pubmed , Xenbase