Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
???displayArticle.abstract???
Oncoprotein 18/Stathmin (Op18) is a microtubule-destabilizing protein that is inhibited by phosphorylation in response to many types of signals. During mitosis, phosphorylation of Op18 by cdc2 is necessary but not sufficient for Op18 inhibition. The presence of mitotic chromosomes is additionally required and involves phosphorylation of Ser-16 in Xenopus Op18 (and/or Ser-63 in human). Given that Ser-16 is an excellent Aurora A (Aur-A) kinase consensus phosphorylation site and the Aurora kinase inhibitor ZM447439 (ZM) blocks phosphorylation in the activation loop of Aur-A, we asked whether either Aur-A or Aurora B (Aur-B) might regulate Op18. We find that ZM blocks the ability of mitotic chromatin to induce Op18 hyperphosphorylation in Xenopus egg extracts. Depletion of Aur-B, but not Aur-A, blocks hyperphosphorylation of Op18, and chromatin assembled in the absence of Aur-B fails to induce hyperphosphorylation. These results suggest that Aur-B, which concentrates at centromeres of metaphase chromosomes, contributes to localized regulation of Op18 during the process of spindle assembly.
Andersen,
Spindle assembly and the art of regulating microtubule dynamics by MAPs and Stathmin/Op18.
2000, Pubmed
Andersen,
Spindle assembly and the art of regulating microtubule dynamics by MAPs and Stathmin/Op18.
2000,
Pubmed Andersen,
Mitotic chromatin regulates phosphorylation of Stathmin/Op18.
1997,
Pubmed
,
Xenbase Andrésson,
The kinase Eg2 is a component of the Xenopus oocyte progesterone-activated signaling pathway.
1998,
Pubmed
,
Xenbase Andrews,
Aurora B regulates MCAK at the mitotic centromere.
2004,
Pubmed Belmont,
Identification of a protein that interacts with tubulin dimers and increases the catastrophe rate of microtubules.
1996,
Pubmed
,
Xenbase Brattsand,
Cell-cycle-regulated phosphorylation of oncoprotein 18 on Ser16, Ser25 and Ser38.
1994,
Pubmed Budde,
Regulation of Op18 during spindle assembly in Xenopus egg extracts.
2001,
Pubmed
,
Xenbase Carazo-Salas,
Generation of GTP-bound Ran by RCC1 is required for chromatin-induced mitotic spindle formation.
1999,
Pubmed
,
Xenbase Carmena,
The cellular geography of aurora kinases.
2003,
Pubmed
,
Xenbase Cassimeris,
The oncoprotein 18/stathmin family of microtubule destabilizers.
2002,
Pubmed Cheeseman,
Phospho-regulation of kinetochore-microtubule attachments by the Aurora kinase Ipl1p.
2002,
Pubmed Curmi,
Stathmin and its phosphoprotein family: general properties, biochemical and functional interaction with tubulin.
1999,
Pubmed Ditchfield,
Aurora B couples chromosome alignment with anaphase by targeting BubR1, Mad2, and Cenp-E to kinetochores.
2003,
Pubmed Ducat,
Aurora kinases in spindle assembly and chromosome segregation.
2004,
Pubmed Eyers,
A novel mechanism for activation of the protein kinase Aurora A.
2003,
Pubmed
,
Xenbase Ferrari,
Aurora-A site specificity: a study with synthetic peptide substrates.
2005,
Pubmed Ferrell,
Xenopus oocyte maturation: new lessons from a good egg.
1999,
Pubmed
,
Xenbase Frank-Vaillant,
Progesterone regulates the accumulation and the activation of Eg2 kinase in Xenopus oocytes.
2000,
Pubmed
,
Xenbase Gadde,
Mechanisms and molecules of the mitotic spindle.
2004,
Pubmed Gadea,
Aurora kinase inhibitor ZM447439 blocks chromosome-induced spindle assembly, the completion of chromosome condensation, and the establishment of the spindle integrity checkpoint in Xenopus egg extracts.
2005,
Pubmed
,
Xenbase Goto,
Complex formation of Plk1 and INCENP required for metaphase-anaphase transition.
2006,
Pubmed Gruss,
Ran induces spindle assembly by reversing the inhibitory effect of importin alpha on TPX2 activity.
2001,
Pubmed
,
Xenbase Heald,
Self-organization of microtubules into bipolar spindles around artificial chromosomes in Xenopus egg extracts.
1996,
Pubmed
,
Xenbase Howell,
Dissociation of the tubulin-sequestering and microtubule catastrophe-promoting activities of oncoprotein 18/stathmin.
1999,
Pubmed Jourdain,
Stathmin: a tubulin-sequestering protein which forms a ternary T2S complex with two tubulin molecules.
1997,
Pubmed Kalab,
The ran GTPase regulates mitotic spindle assembly.
1999,
Pubmed
,
Xenbase Kallio,
Inhibition of aurora B kinase blocks chromosome segregation, overrides the spindle checkpoint, and perturbs microtubule dynamics in mitosis.
2002,
Pubmed
,
Xenbase Karsenti,
The mitotic spindle: a self-made machine.
2001,
Pubmed
,
Xenbase Karsenti,
Role of the centrosome in organizing the interphase microtubule array: properties of cytoplasts containing or lacking centrosomes.
1984,
Pubmed Karsenti,
Respective roles of centrosomes and chromatin in the conversion of microtubule arrays from interphase to metaphase.
1984,
Pubmed
,
Xenbase Keen,
Aurora-kinase inhibitors as anticancer agents.
2004,
Pubmed Kline-Smith,
Depletion of centromeric MCAK leads to chromosome congression and segregation defects due to improper kinetochore attachments.
2004,
Pubmed
,
Xenbase Küntziger,
Stathmin/Op18 phosphorylation is regulated by microtubule assembly.
2001,
Pubmed
,
Xenbase Lan,
Aurora B phosphorylates centromeric MCAK and regulates its localization and microtubule depolymerization activity.
2004,
Pubmed
,
Xenbase Larsson,
Control of microtubule dynamics by oncoprotein 18: dissection of the regulatory role of multisite phosphorylation during mitosis.
1997,
Pubmed Larsson,
G2/M transition requires multisite phosphorylation of oncoprotein 18 by two distinct protein kinase systems.
1995,
Pubmed Littlepage,
Identification of a new APC/C recognition domain, the A box, which is required for the Cdh1-dependent destruction of the kinase Aurora-A during mitotic exit.
2002,
Pubmed
,
Xenbase Littlepage,
Identification of phosphorylated residues that affect the activity of the mitotic kinase Aurora-A.
2002,
Pubmed
,
Xenbase Manna,
Stathmin strongly increases the minus end catastrophe frequency and induces rapid treadmilling of bovine brain microtubules at steady state in vitro.
2006,
Pubmed Marklund,
Oncoprotein 18 is a phosphorylation-responsive regulator of microtubule dynamics.
1996,
Pubmed Murnion,
Chromatin-associated protein phosphatase 1 regulates aurora-B and histone H3 phosphorylation.
2001,
Pubmed
,
Xenbase Nachury,
Importin beta is a mitotic target of the small GTPase Ran in spindle assembly.
2001,
Pubmed
,
Xenbase Nakajima,
Identification of a consensus motif for Plk (Polo-like kinase) phosphorylation reveals Myt1 as a Plk1 substrate.
2003,
Pubmed
,
Xenbase Niethammer,
Stathmin-tubulin interaction gradients in motile and mitotic cells.
2004,
Pubmed
,
Xenbase Ohba,
Self-organization of microtubule asters induced in Xenopus egg extracts by GTP-bound Ran.
1999,
Pubmed
,
Xenbase Ohi,
Differentiation of cytoplasmic and meiotic spindle assembly MCAK functions by Aurora B-dependent phosphorylation.
2004,
Pubmed
,
Xenbase Pinsky,
The spindle checkpoint: tension versus attachment.
2005,
Pubmed Sampath,
The chromosomal passenger complex is required for chromatin-induced microtubule stabilization and spindle assembly.
2004,
Pubmed
,
Xenbase Sarkissian,
Progesterone and insulin stimulation of CPEB-dependent polyadenylation is regulated by Aurora A and glycogen synthase kinase-3.
2004,
Pubmed
,
Xenbase Sobel,
Stathmin: a relay phosphoprotein for multiple signal transduction?
1991,
Pubmed Tanaka,
Chromosome bi-orientation on the mitotic spindle.
2005,
Pubmed Tournebize,
Control of microtubule dynamics by the antagonistic activities of XMAP215 and XKCM1 in Xenopus egg extracts.
2000,
Pubmed
,
Xenbase Tsai,
A Ran signalling pathway mediated by the mitotic kinase Aurora A in spindle assembly.
2003,
Pubmed
,
Xenbase Tsai,
Aurora A kinase-coated beads function as microtubule-organizing centers and enhance RanGTP-induced spindle assembly.
2005,
Pubmed
,
Xenbase Vagnarelli,
Chromosomal passengers: the four-dimensional regulation of mitotic events.
2004,
Pubmed Varmark,
Functional role of centrosomes in spindle assembly and organization.
2004,
Pubmed Walczak,
XKCM1: a Xenopus kinesin-related protein that regulates microtubule dynamics during mitotic spindle assembly.
1996,
Pubmed
,
Xenbase Walter,
The mitotic serine/threonine kinase Aurora2/AIK is regulated by phosphorylation and degradation.
2000,
Pubmed Wilde,
Stimulation of microtubule aster formation and spindle assembly by the small GTPase Ran.
1999,
Pubmed
,
Xenbase Yang,
Mitotic requirement for aurora A kinase is bypassed in the absence of aurora B kinase.
2005,
Pubmed Zhao,
The GIT-associated kinase PAK targets to the centrosome and regulates Aurora-A.
2005,
Pubmed
