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Endocrine disrupting chemicals (EDCs) can perturb the hypothalamic-pituitary-thyroid axis affecting human and wildlife health. Thyroid hormones (TH) are crucial regulators of metabolism, growth, and differentiation. The perinatal stage is most reliant on TH, thus vulnerable to TH disrupting chemicals. Dysregulation of TH signaling during perinatal development can weaken T cell function in maturity, raising the question of whether TH disrupting chemicals can perturb thymocyte development. Using Xenopus laevis tadpoles as model, we determined TH disrupting effects and thymocyte alterations following exposure to a mixture of common waterborne TH disrupting chemicals at concentrations similar to those found in contaminated water. This mixture included naphthalene, ethylene glycol, ethoxylated nonylphenol, and octylphenol, which have documented TH disrupting activity. Besides hypertrophy-like pathology in the thyroid gland and delayed metamorphosis, exposure to the mixture antagonized TH receptor-induced transcription of the Krüppel-like factor 9 transcription factor and significantly raised thyroid-stimulating hormone gene expression in the brain, two genes that modulate thymocyte differentiation. Importantly, exposure to this mixture reduced the number of Xenopus immature cortical thymocyte-specific-antigen (CTX+) and mature CD8+ thymocytes, whereas co-exposure with exogenous TH (T3) abolished the effect. When each chemical of the mixture was individually tested, only ethylene glycol induced significant antagonist effects on brain, thymic gene expression, and CD8+ thymocytes. These results suggest that EDCs in mixture are more potent than each chemical alone to perturb thymocyte development through TH-dependent pathway, and provide a starting point to research TH influence on thymocyte development.
Albornoz,
Gestational hypothyroidism increases the severity of experimental autoimmune encephalomyelitis in adult offspring.
2013, Pubmed
Albornoz,
Gestational hypothyroidism increases the severity of experimental autoimmune encephalomyelitis in adult offspring.
2013,
Pubmed Ashwell,
Cross-talk between the T cell antigen receptor and the glucocorticoid receptor regulates thymocyte development.
1996,
Pubmed Barber,
GAPDH as a housekeeping gene: analysis of GAPDH mRNA expression in a panel of 72 human tissues.
2005,
Pubmed Buchholz,
Xenopus metamorphosis as a model to study thyroid hormone receptor function during vertebrate developmental transitions.
2017,
Pubmed
,
Xenbase Calsolaro,
Thyroid Disrupting Chemicals.
2017,
Pubmed Capen,
Mechanisms of chemical injury of thyroid gland.
1994,
Pubmed Carvalho,
Thyroid hormone biosynthesis and release.
2017,
Pubmed Degitz,
Progress towards development of an amphibian-based thyroid screening assay using Xenopus laevis. Organismal and thyroidal responses to the model compounds 6-propylthiouracil, methimazole, and thyroxine.
2005,
Pubmed
,
Xenbase Denver,
Acceleration of anuran amphibian metamorphosis by corticotropin-releasing hormone-like peptides.
1993,
Pubmed Ehrenkranz,
Circadian and Circannual Rhythms in Thyroid Hormones: Determining the TSH and Free T4 Reference Intervals Based Upon Time of Day, Age, and Sex.
2015,
Pubmed Even-Desrumeaux,
Affinity determination of biotinylated antibodies by flow cytometry.
2012,
Pubmed Famili,
The development of T cells from stem cells in mice and humans.
2017,
Pubmed Fini,
[Thyroid disruptors and their consequences on brain development and behavior].
2019,
Pubmed Fini,
Human amniotic fluid contaminants alter thyroid hormone signalling and early brain development in Xenopus embryos.
2017,
Pubmed
,
Xenbase Geenen,
[History of the thymus: from an "accident of evolution" to the programming of immunological self-tolerance].
2017,
Pubmed Grim,
Thyroid histopathology assessments for the amphibian metamorphosis assay to detect thyroid-active substances.
2009,
Pubmed
,
Xenbase Hu,
A Mechanism to Enhance Cellular Responsivity to Hormone Action: Krüppel-Like Factor 9 Promotes Thyroid Hormone Receptor-β Autoinduction During Postembryonic Brain Development.
2016,
Pubmed
,
Xenbase Jie,
Pollution by Nonylphenol in river, tap water, and aquatic in an acid rain-plagued city in southwest China.
2017,
Pubmed Kassotis,
Endocrine-Disrupting Activity of Hydraulic Fracturing Chemicals and Adverse Health Outcomes After Prenatal Exposure in Male Mice.
2015,
Pubmed Kulkarni,
Corticosteroid signaling in frog metamorphosis.
2014,
Pubmed Livak,
Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method.
2001,
Pubmed Manley,
Hox group 3 paralogs regulate the development and migration of the thymus, thyroid, and parathyroid glands.
1998,
Pubmed
,
Xenbase Miyata,
Thyroid Hormone-disrupting Effects and the Amphibian Metamorphosis Assay.
2012,
Pubmed
,
Xenbase Navarro,
Real-time PCR detection chemistry.
2015,
Pubmed Opitz,
Evaluation of histological and molecular endpoints for enhanced detection of thyroid system disruption in Xenopus laevis tadpoles.
2006,
Pubmed
,
Xenbase Preuss,
Naphthalene--an environmental and occupational toxicant.
2003,
Pubmed Robert,
Water Contaminants Associated With Unconventional Oil and Gas Extraction Cause Immunotoxicity to Amphibian Tadpoles.
2018,
Pubmed
,
Xenbase Robert,
In vitro thymocyte differentiation in MHC class I-negative Xenopus larvae.
2001,
Pubmed
,
Xenbase Robert,
Comparative and developmental study of the immune system in Xenopus.
2009,
Pubmed
,
Xenbase Robert,
Developmental exposure to chemicals associated with unconventional oil and gas extraction alters immune homeostasis and viral immunity of the amphibian Xenopus.
2019,
Pubmed
,
Xenbase Robert,
In vitro differentiation of a CD4/CD8 double-positive equivalent thymocyte subset in adult Xenopus.
1999,
Pubmed
,
Xenbase Rollins-Smith,
Expression of class II major histocompatibility complex antigens on adult T cells in Xenopus is metamorphosis-dependent.
1990,
Pubmed
,
Xenbase Rollins-Smith,
Involvement of glucocorticoids in the reorganization of the amphibian immune system at metamorphosis.
1997,
Pubmed
,
Xenbase Rollins-Smith,
Thymus ontogeny in frogs: T-cell renewal at metamorphosis.
1992,
Pubmed
,
Xenbase Schneider,
NIH Image to ImageJ: 25 years of image analysis.
2012,
Pubmed Silva,
Something from "nothing"--eight weak estrogenic chemicals combined at concentrations below NOECs produce significant mixture effects.
2002,
Pubmed Thambirajah,
Contaminant and Environmental Influences on Thyroid Hormone Action in Amphibian Metamorphosis.
2019,
Pubmed Tran,
Determination of trace amounts of ethylene glycol and its analogs in water matrixes by liquid chromatography/tandem mass spectrometry.
2014,
Pubmed Versieren,
Mixture toxicity and interactions of copper, nickel, cadmium, and zinc to barley at low effect levels: Something from nothing?
2016,
Pubmed Wang,
Influence of nonylphenol exposure on basic growth, development, and thyroid tissue structure in F1 male rats.
2019,
Pubmed Wu,
Thyrotropin Alters T Cell Development in the Thymus in Subclinical Hypothyroidism Mouse Model.
2017,
Pubmed Xie,
Octylphenol influence growth and development of Rana chensinensis tadpoles via disrupting thyroid function.
2019,
Pubmed Yang,
Determination of 4-tert-octylphenol in surface water samples of Jinan in China by solid phase extraction coupled with GC-MS.
2013,
Pubmed Yao,
Optimization of the T3-induced Xenopus metamorphosis assay for detecting thyroid hormone signaling disruption of chemicals.
2017,
Pubmed
,
Xenbase Zhang,
Thyroid hormone regulates hematopoiesis via the TR-KLF9 axis.
2017,
Pubmed
