Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Nat Genet
2021 Jun 01;536:906-915. doi: 10.1038/s41588-021-00855-6.
Show Gene links
Show Anatomy links
A plasma membrane transporter coordinates phosphate reallocation and grain filling in cereals.
Ma B, Zhang L, Gao Q, Wang J, Li X, Wang H, Liu Y, Lin H, Liu J, Wang X, Li Q, Deng Y, Tang W, Luan S, He Z.
???displayArticle.abstract???
Phosphate (Pi) is essential to plant growth and crop yield. However, it remains unknown how Pi homeostasis is maintained during cereal grain filling. Here, we identified a rice grain-filling-controlling PHO1-type Pi transporter, OsPHO1;2, through map-based cloning. Pi efflux activity and its localization to the plasma membrane of seed tissues implicated a specific role for OsPHO1;2 in Pi reallocation during grain filling. Indeed, Pi over-accumulated in developing seeds of the Ospho1;2 mutant, which inhibited the activity of ADP-glucose pyrophosphorylase (AGPase), important for starch synthesis, and the grain-filling defect was alleviated by overexpression of AGPase in Ospho1;2-mutant plants. A conserved function was recognized for the maize transporter ZmPHO1;2. Importantly, ectopic overexpression of OsPHO1;2 enhanced grain yield, especially under low-Pi conditions. Collectively, we discovered a mechanism underlying Pi transport, grain filling and P-use efficiency, providing an efficient strategy for improving grain yield with minimal P-fertilizer input in cereals.
Arpat,
Functional expression of PHO1 to the Golgi and trans-Golgi network and its role in export of inorganic phosphate.
2012, Pubmed
Arpat,
Functional expression of PHO1 to the Golgi and trans-Golgi network and its role in export of inorganic phosphate.
2012,
Pubmed Ballicora,
ADP-Glucose Pyrophosphorylase: A Regulatory Enzyme for Plant Starch Synthesis.
2004,
Pubmed Chen,
Nuclear-encoded synthesis of the D1 subunit of photosystem II increases photosynthetic efficiency and crop yield.
2020,
Pubmed Fujita,
Purification, characterization, and cDNA structure of isoamylase from developing endosperm of rice.
1999,
Pubmed Gu,
Complex Regulation of Plant Phosphate Transporters and the Gap between Molecular Mechanisms and Practical Application: What Is Missing?
2016,
Pubmed Hansen,
Micro-scaled high-throughput digestion of plant tissue samples for multi-elemental analysis.
2009,
Pubmed Jeon,
Starch biosynthesis in cereal endosperm.
2010,
Pubmed Kim,
Synchrotron X-ray imaging for nondestructive monitoring of sap flow dynamics through xylem vessel elements in rice leaves.
2010,
Pubmed Kopriva,
Are we ready to improve phosphorus homeostasis in rice?
2018,
Pubmed Krishnan,
Structural and histochemical studies on grain-filling in the caryopsis of rice (Oryza sativa L.).
2003,
Pubmed Lee,
Identification of the ADP-glucose pyrophosphorylase isoforms essential for starch synthesis in the leaf and seed endosperm of rice (Oryza sativa L.).
2007,
Pubmed Liu,
A vacuolar phosphate transporter essential for phosphate homeostasis in Arabidopsis.
2015,
Pubmed Młodzińska,
Phosphate Uptake and Allocation - A Closer Look at Arabidopsis thaliana L. and Oryza sativa L.
2016,
Pubmed Nishi,
Biochemical and genetic analysis of the effects of amylose-extender mutation in rice endosperm.
2001,
Pubmed Pan,
Dynamic Interactions of Plant CNGC Subunits and Calmodulins Drive Oscillatory Ca2+ Channel Activities.
2019,
Pubmed Secco,
Characterization of the rice PHO1 gene family reveals a key role for OsPHO1;2 in phosphate homeostasis and the evolution of a distinct clade in dicotyledons.
2010,
Pubmed Sosso,
Seed filling in domesticated maize and rice depends on SWEET-mediated hexose transport.
2015,
Pubmed Stefanovic,
Members of the PHO1 gene family show limited functional redundancy in phosphate transfer to the shoot, and are regulated by phosphate deficiency via distinct pathways.
2007,
Pubmed Sun,
A constitutive expressed phosphate transporter, OsPht1;1, modulates phosphate uptake and translocation in phosphate-replete rice.
2012,
Pubmed Sun,
Sugar homeostasis mediated by cell wall invertase GRAIN INCOMPLETE FILLING 1 (GIF1) plays a role in pre-existing and induced defence in rice.
2014,
Pubmed Tagashira,
Overexpression of a Gene Involved in Phytic Acid Biosynthesis Substantially Increases Phytic Acid and Total Phosphorus in Rice Seeds.
2015,
Pubmed Tang,
ADP-glucose pyrophosphorylase large subunit 2 is essential for storage substance accumulation and subunit interactions in rice endosperm.
2016,
Pubmed Tian,
Allelic diversities in rice starch biosynthesis lead to a diverse array of rice eating and cooking qualities.
2009,
Pubmed Tuncel,
The rice endosperm ADP-glucose pyrophosphorylase large subunit is essential for optimal catalysis and allosteric regulation of the heterotetrameric enzyme.
2014,
Pubmed Tuncel,
Improving starch yield in cereals by over-expression of ADPglucose pyrophosphorylase: expectations and unanticipated outcomes.
2013,
Pubmed Vogiatzaki,
PHO1 Exports Phosphate from the Chalazal Seed Coat to the Embryo in Developing Arabidopsis Seeds.
2017,
Pubmed Wang,
Rice SPX-Major Facility Superfamily3, a Vacuolar Phosphate Efflux Transporter, Is Involved in Maintaining Phosphate Homeostasis in Rice.
2015,
Pubmed
,
Xenbase Wang,
Enhanced Sucrose Loading Improves Rice Yield by Increasing Grain Size.
2015,
Pubmed Wang,
Control of rice grain-filling and yield by a gene with a potential signature of domestication.
2008,
Pubmed Wang,
Roles, Regulation, and Agricultural Application of Plant Phosphate Transporters.
2017,
Pubmed Wykoff,
Phosphate transport and sensing in Saccharomyces cerevisiae.
2001,
Pubmed Yamaji,
Spatial distribution and temporal variation of the rice silicon transporter Lsi1.
2007,
Pubmed Yamaji,
Reducing phosphorus accumulation in rice grains with an impaired transporter in the node.
2017,
Pubmed Yang,
Grain filling of cereals under soil drying.
2006,
Pubmed Yoshida,
Temporal and spatial patterns of accumulation of the transcript of Myo-inositol-1-phosphate synthase and phytin-containing particles during seed development in rice.
1999,
Pubmed Zhang,
Knockdown of Rice MicroRNA166 Confers Drought Resistance by Causing Leaf Rolling and Altering Stem Xylem Development.
2018,
Pubmed
